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Liebhard, R.; Gianfranceschi, L.; Koller, Bernhard; Ryder, Carol D.; Tarchini, Renato; Weg, Eric van de; Gessler, C.

doi:10.1023/a:1020525906332

Cited by 461 publications

(62 citation statements)

References 30 publications

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“…Clonal diversity in peripheral populations of wild service tree (Oddou-Muratorio et al 2001b); CH01h01 (Gianfranceschi et al 1998); CH02c09, Ms14H03 (Liebhard et al 2002). Two PCR multiplexes were designed and optimized including six Ch02c09,Mss4,Sa01,Sa07,Sa14) and seven loci Mss1,Mss5,Mss6,Mss9,Mss13,Mss16 (Guichoux et al 2011).…”

Section: Iforest -Biogeosciences and Forestrymentioning

confidence: 99%

Clonal structure and high genetic diversity at peripheral populations of Sorbus torminalis (L.) Crantz.

Jankowska-Wróblewska¹,

Meyza²,

Sztupecka³

et al. 2016

iForest

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Knowing the level of genetic diversity and structure in marginal plant populations is essential for managing their genetic resources. This is particularly important for rare scattered tree species, such as Sorbus torminalis (L.) Crantz. We investigated the genetic diversity and its spatial distribution in peripheral populations of S. torminalis. As the species is known to reproduce vegetatively, we also evaluated clonal structure within populations. Using 13 nuclear microsatellite loci designed in two multiplexes, we genotyped 172 individuals revealing the existence of 100 distinct genotypes. Number of ramets per genotype was variable across populations with an average of 1.72. Examples of somaclonal variation at particular loci were detected. Measures of genetic diversity of the total sample were relatively high (mean allelic richness AR = 10.293; expected heterozygosity He = 0.756), as compared to other S. torminalis populations. We noticed a slightly negative inbreeding coefficient (FIS = -0.029) indicating a small excess of heterozygotes, which is typical for self-incompatible plants. Genetic differentiation among populations was low (FST = 0.048), but Bayesian clustering methods revealed the existence of three distinct genetic clusters only in part related to population structure. Significant spatial genetic structure within populations was also detected (Sp = 0.0125) indicating fine-scale pattern of isolation by distance. Our study demonstrated that peripheral populations of S. torminalis may exhibit relatively high levels of genetic diversity despite the existence of vegetative propagation. Nevertheless, if the studied or similar populations are expected to be utilized as seed sources for ex-situ or in-situ conservation purposes, the existence of clonal structure and spatial genetic structure must be taken into account in order to avoid excessive sampling of the same or closely related genets.

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Section: Iforest -Biogeosciences and Forestrymentioning

confidence: 99%

Clonal structure and high genetic diversity at peripheral populations of Sorbus torminalis (L.) Crantz.

Jankowska-Wróblewska¹,

Meyza²,

Sztupecka³

et al. 2016

iForest

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“…(Zhen et al 2004). These markers have proved advantageous for diversity studies on apple (Garkava-Gustavsson et al 2008;Gasi et al 2010;Gross et al 2014;Hokanson et al 2001;Liang et al 2015;Moriya et al 2011;Pereira-Lorenzo et al 2008;Song et al 2006;Urrestarazu et al 2012;van Treuren et al 2010), and several hundred SSR markers have been developed and genetically mapped across the 17 linkage groups of the apple genome (Gianfranceschi et al 1998;Liebhard et al 2002;Silfverberg-Dilworth et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity, Population Structure, Parentage Analysis, and Construction of Core Collections in the French Apple Germplasm Based on SSR Markers

et al. 2016

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In-depth characterization of apple genetic resources is a prerequisite for genetic improvement and for germplasm management. In this study, we fingerprinted a very large French collection of 2163 accessions with 24 SSR markers in order to evaluate its genetic diversity, population structure, and genetic relationships, to link these features with cultivar selection date or usage (old or modern, dessert or cider cultivars), and to construct core collections. Most markers were highly discriminating and powerful for varietal identification, with a probability of identity P (ID) over the 21 retained SSR loci close to 10 −28. Pairwise comparisons revealed 34 % redundancy and 18.5 % putative triploids. The results showed that the germplasm is highly diverse with an expected heterozygosity H e of 0.82 and observed heterozygosity H o of 0.83. A Bayesian model-based clustering approach revealed a weak but significant structure in three subgroups (F ST = 0.014-0.048) corresponding, albeit approximately, to the three subpopulations defined beforehand (Old Dessert, Old Cider, and Modern Cultivars). Parentage analyses established already known and yet unknown relationships, notably between old cultivars, with the frequent occurrence of cultivars such as BKing of Pippin^and BCalville Rouge d'Hiver^as founders. Finally, core collections based on allelic diversity were constructed. A large dessert core collection of 278 cultivars contained 90 % of the total dessert allelic diversity, whereas a dessert subcore collection of 48 cultivars contained 71 % of diversity. For cider apples, a 48-cultivar core collection contained 83 % of the total cider allelic diversity.

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“…Our results demonstrate that 27 SSR markers of three linkage groups localised on 'Fiesta' and 'Discovery' maps (Liebhard et al 2002, 2003, Silfverberg-Dilworth et al 2006) are also present in 'Retina' and 'Topaz', cultivars that are characterised by high phenotypic diversity. The summer cultivar 'Retina' was developed in 1982 in DresdenPillnitz as a lineal offspring of 'Apollo' and clone BX 44/2.…”

Section: Discussionmentioning

confidence: 68%

“…Additional 27 markers, distributed on three linkage groups of reference 'Fiesta' × 'Discovery' map as follows: LG2 -7 SSRs LG12 -10 SSRs and LG14 -10 SSRs (Liebhard et al 2002(Liebhard et al , 2003 (Table 1), were used to gene-rate the skeleton of 'Retina' and 'Topaz' genetic map. PCR was performed in Biometra Basic thermocycler according to the following thermal profile: 96 °C for 30 s, four steps with reduced annealing temperature, starting from 65 °C for 90 s, followed by 30 cycles with annealing temperature of 60 °C for 90 s. PCR products were electrophoretically size fractionated in 7% polyacrylamide gels, containing 0.4% urea and stained with 0.1% of silver nitrate.…”

Section: Ssr (Single Sequence Repeat) Markersmentioning

confidence: 99%

“…Improvement of the mapping technology resulted in maps for many other cultivars including: 'Wijcik McIntosh' (WM), NY 75441-67, NY 75441-58, 'Telamon', 'Braeburn', 'Prima', 'Fiesta', 'Discovery', 'Ralls Janet', 'Delicious', covering from 842 cM (Conner et al 1997, Maliepaard et al 1998, Kenis & Keulemans 2005, Igarashi et al 2008 to almost 2000 cM in integrated maps (N'Diaye et al 2008. The maps were constructed and saturated using RAPD (Random Amplified Polymorphic DNA), RFLP (Restriction Fragment Length Polymorphism), AFLP (Amplified Fragment Length Polymorphism) (Hemmat et al 1994, Maliepaard et al 1998, Liebhard et al 2002, 2003, and more recently SSR (Simple Sequence Repeat), SNP (Single Nucleotide Polymorphism) (Silfverberg-Dilworth et al 2006, Igarashi et al 2008, cDNA microarray (Soglio et al 2009) and diversity array technology (DArT) based markers (Schouten et al 2012). Among them many sequences were linked to economically important traits such as plant resistance to pests and diseases, plant growth habit, fruit colour, acidity and sweet taste (Korban & Tartarini 2009).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Four New Est-Based Markers on the Apple (MALUS ⋉ DOMESTICA) Genetic Map

Keller-Przybyłkowicz

Korbin

2014

Journal of Horticultural Research

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The map of the linkage groups: LG2, LG12 and LG14, which are expected to contain QTLs related to fruit quality, was generated by analysis of 56 individuals of the cross: 'Retina' × 'Topaz'. Twenty three of the 27 SSR markers covered 225 cM in 'Retina' and 371 cM in 'Topaz' genome. High level of colinearity (~ 85%) was found between obtained map and the respective map regions of 'Fiesta', 'Discovery', 'Ralls Janet' and 'Delicious'. Only single inversions of marker positions were noted, predominantly in 'Topaz'. CAPS and SSCP/SNP markers in seven ESTs, chosen based on the metabolic pathways of ascorbic acids and sugar, were identified. Four of the CAPS markers, linked to the genes coding: UDP glucose : flavonoid 3-o-glucosyl transferase, ascorbate peroxidase, and two sugar transporters, were mapped on LG2 (GFglTra -both cultivars), LG12 (PGiso1B and PSTS -'Topaz') and LG14 (PST -'Retina'). According to our knowledge, loci of these markers have never been identified on the apple genome map.

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Cited by 461 publications

References 30 publications

Clonal structure and high genetic diversity at peripheral populations of Sorbus torminalis (L.) Crantz.

Clonal structure and high genetic diversity at peripheral populations of Sorbus torminalis (L.) Crantz.

Genetic Diversity, Population Structure, Parentage Analysis, and Construction of Core Collections in the French Apple Germplasm Based on SSR Markers

Identification of Four New Est-Based Markers on the Apple (MALUS ⋉ DOMESTICA) Genetic Map

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