Untitled

Abstract

A 60-day feeding trial was conducted to study the nutrient utilization and growth performance of Labeo rohita fed with T1 [DORB supplemented with phytase and xylanase (0.01% each)], T2 [ T1+ L-lysine(1.4%), L-methionine (0.4%), combination of EPA and DHA (0.5%)] , T3 [DORB supplemented with phytase (0.01%), combination of xylanase and cellulase (0.075%), L-lysine (1.4 %), L-methionine (0.4 %), combination of EPA and DHA (0.5%)] and T4 (commercially available carp feed) diets. Weight gain%, SGR, and FCR were similar (p>0.05) for the fishes fed with either T3 feed or commercial carp feed (T4), whereas PER was highest for the T3 group. A significantly higher apparent dry matter and carbohydrate digestibility were observed in the T3 group. Highest crude protein digestibility was observed in T4 group, though lipid digestibility was similar in both T3 and T4 groups. The activities of digestive enzymes were similar in T2, T3 and T4 groups, whereas the lowest activity of these enzymes were observed in T1 group. Based on the findings it is concluded that T3 feed (crude protein 18.18 %) is having comparable growth performance of Labeo rohita as compared to T4 diet (crude protein 32.01 %) due to higher In vivo digestibility.

Green pea, Pisum sativum leaf meal with supplemented exogenous cellulase and xylanase can completely replace de‐oiled rice bran (DORB) in the diet of Labeo rohita (Hamilton, 1878) fingerlings

“…Since exogenous NSPase could improve the crude fibre digestion and lead to more amount of soluble carbohydrate in the intestine, it might have stimulated the pancreas for release of more amylase (Li et al, 2009). In corroboration with the present finding, Ranjan et al (2019) observed improved dry matter digestibility of DORB-based diet in fish due to supplementation of exogenous enzymes. Moreover, in agreement with our observation, Shi et al (2017) reported that supplementation of carbohydrases at 1 g/kg diet of crucian carp (Carassius auratus) did not affect ACPDC and ALDC.…”

“…the negative effect of the ANFs present in PSLM. Moreover, in the present study, dietary supplementation of exogenous cellulase and xylanase mixture at 1 g/kg in low PSLM group (150 g PSLM/kg) significantly improved the whole-body protein content, while fish fed with exogenous enzyme and supplemented with high PSLM (300 g PSLM/kg) could not show a significant difference of whole-body protein content compared to their non-supplemented counterparts.In agreement with the present observation,Ranjan et al (2019) reported that carcass protein content of L. rohita fed with DORB-based diets was not altered due to dietary addition of NSP degrading enzymes. However, in contrast to our finding,Ai et al (2007) reported that supplementation of NSP degrading enzyme in plant-based diet could enhance the carcass protein content in Japanese seabass.…”

“…Though plant-based ingredients have limitations for their optimum inclusion in the aquafeed due to the presence of antinutritional factors (ANFs). Several approaches such as moist heat treatments, water soaking, fermentation and exogenous enzyme supplementation have been used to reduce the effect of ANFs present in leaf meals (Ahmad et al, 2019;Maiti et al, 2019;Meshram et al, 2018;Ranjan et al, 2019). Additionally, plant ingredients contain non-starch polysaccharides (NSPs), whose digestibility is low in fish due to lack of NSP digesting enzymes.…”

“…Additionally, plant ingredients contain non-starch polysaccharides (NSPs), whose digestibility is low in fish due to lack of NSP digesting enzymes. Use of exogenous carbohydrases in plant-based feed is an effective method to reduce the NSP (Ranjan et al, 2019). Exogenous cellulase and xylanase are reported to reduce the molecular size and viscosity of NSPs in the gut leading to enhanced digestion, growth and nutrient utilization of fish (Kumar et al, 2006;Kumar et al, 2009;Ranjan et al, 2019).…”

Substitution of de‐oiled rice bran with Khejri pod meal and groundnut oilcake with Khejri seed meal in the diet of Labeo rohita (Hamilton, 1822)

“…DORB contains 15-17% crude protein and 12-16% crude fibre (Ranjan et al, 2017). As DORB is widely distributed, easily available and cheapest, it is most commonly incorporated in fish farm-made feed along with different oilcakes (Meshram et al, 2018;Ranjan et al, 2017Ranjan et al, , 2018Ranjan et al, , 2019. However, recent studies indicated that 33-40% inclusion of DORB in the carp diet does not affect the growth in rohu fingerlings (Kumar et al, , 2019.…”