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Abstract

ABSTRACT:The aim of this study was to explore a novel microbial enzyme was screened, optimized, characterized and purified from the local soil gram positive and spore forming bacterium and to study their ability to degrade chicken feathers and pretreatment of industrial residues from leather industry. After different biochemical and microbiological tests it was suggested that the organism was Bacillus subtilis. It was also characterized and identified by using a bioinformatics tool PIB that suggests the organism was B. subtilis. The isoelectric point was 5.1 and the optimum temperature for enzyme activity was at 60°C but they can produce feather degrading enzymes at 53°C and at pH 8.5. Native enzyme preparations typically showed a Km and Vmax of 0.40mM and 12,200 U mg -1 , respectively. The proteolytic activity was 21.13 units for the sample.This enzyme was purified by ammonium sulfate fractionation, dialysis, DEAE cellulose chromatography and electrophoretic analysis. The most active enzyme protein preparation could be obtained at the ammonium sulphate level of 60%. In this process the protein were purified to 4.9 fold. Enzymatic dehairing estimation has shown that culture supernatant could dehair of leather completely in a 9 hours of incubation. In future the tanneries will use a combination of chemical and enzymatic processes. The potential use of microbial enzymes in leather and poultry processing industry for promoting the hydrolysis of proteins that showing significant industrial and biotechnological applications.