Untitled

Hill, Donna R.; Keenan, T.W.; Helm, Richard F.; Potts, Malcolm; Crowe, Lois M.; Crowe, John H.

doi:10.1023/a:1007965229567

Cited by 124 publications

(16 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is further concluded that a critical feature of the dynamics of these processes is the specific location of WspA, at the interface of a gel-sol transition boundary close to the cell surface ( Fig. 7D) (8,10,34). The extremely hydrophilic nature of the N terminus of WspA suggests a possible mechanistic basis for its orientation in this transition zone which, on the basis of the volatile nature of the latter during critical point drying, was suggested to be the last repository of liquid, in otherwise desiccated colonies (34).…”

Section: Discussionmentioning

confidence: 96%

“…7D) (8,10,34). The extremely hydrophilic nature of the N terminus of WspA suggests a possible mechanistic basis for its orientation in this transition zone which, on the basis of the volatile nature of the latter during critical point drying, was suggested to be the last repository of liquid, in otherwise desiccated colonies (34). It should be noted, however, that although biochemical and regulatory properties of WspA were identified, the role of the protein in stress tolerance is not strictly identified, because a transformation system with which to inactivate wspA in N. commune DRH1, is not currently available.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

UV Irradiation and Desiccation Modulate the Three-dimensional Extracellular Matrix of Nostoc commune (Cyanobacteria)

Wright

Smith

Joardar

et al. 2005

Journal of Biological Chemistry

Self Cite

112

105

View full text Add to dashboard Cite

Cyanobacterium Nostoc commune can tolerate the simultaneous stresses of desiccation, UV irradiation, and oxidation. Acidic WspA, of ϳ33.6 kDa, is secreted to the three-dimensional extracellular matrix and accounts for greater than 70% of the total soluble protein. The wspA gene of N. commune strain DRH1 was cloned and found in a single genomic copy, in a monocistronic operon. Transcription of wspA and sodF (superoxide dismutase), and synthesis and secretion of WspA, were induced upon desiccation or UV-A/B irradiation of cells. Recombinant WspA binds the UV-A/B absorbing pigment scytonemin through non-covalent interactions. WspA peptide polymorphism, and heterogeneity of multiple wspA sequences within cells of a single colony, account for distinct WspA isoforms. WspA has no similarity to entries in the sequence databases and wspA, a possible xenolog, is restricted to a subset of strains in the "form species" N. commune characterized through group I intron phylogeny. We hypothesize that WspA plays a central role in the global stress response of N. commune through modulation of the structure and function of the three-dimensional extracellular matrix, particularly the transport, distribution, and/or macromolecular architecture of mycosporine and scytonemin UV-A/B absorbing pigment complexes.Cyanobacteria make a major contribution to world photosynthesis and nitrogen fixation, but their dense and often toxic "blooms" in marine, brackish, and fresh waters are of growing concern to public health (1). The sequences of a wide range of cyanobacterial genomes are becoming available and constitute an important resource both for the rational management and exploitation of these organisms, as well as the study of topics as diverse as carbon fixation, cell differentiation, plant and fungal symbioses, secondary product biosynthesis, and evolution.

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

UV Irradiation and Desiccation Modulate the Three-dimensional Extracellular Matrix of Nostoc commune (Cyanobacteria)

Wright

Smith

Joardar

et al. 2005

Journal of Biological Chemistry

Self Cite

112

105

View full text Add to dashboard Cite

show abstract

“…17 However, this does not explain why trehalose also shows good stabilization properties at low and medium concentrated solutions (≈0.05-1.0 M) which are natural for living organisms. [18][19][20] Both water replacement and water entrapment hypotheses are supported by different sets of experimental and theoretical data. Infrared spectroscopy experiments 21 show that there is a large number of direct hydrogen bonds formed between trehalose and lysozyme, which is suggestive of the water replacement hypothesis.…”

Section: Introductionmentioning

confidence: 99%

Self-assembly of trehalose molecules on a lysozyme surface: the broken glass hypothesis

Fedorov

Goodman

Nerukh

et al. 2011

Phys. Chem. Chem. Phys.

View full text Add to dashboard Cite

“…There are more than 100 cyanobacteria strains belonging to 20 different genera which have been investigated with regard to the production and the released of extracellular polysaccharides (EPS) into the culture medium [2]. The EPS play an important role in protecting cells from various stresses [3,4]. As a new biomaterial, it was found to have a wide range of applications such as improvement of water-holding capacity of soil, removal of heavy metals from wastewater, and being used as food additives [5][6][7].…”

mentioning

confidence: 99%

Accumulation of Exopolysaccharides in Liquid Suspension Culture of Nostoc flagelliforme Cells

Jia

Dai

2008

Appl Biochem Biotechnol

View full text Add to dashboard Cite

The liquid suspension culture of dissociated Nostoc flagelliforme cells was investigated. It was found that the growth rate of N. flagelliforme cells and the accumulation of exopolysaccharides (EPS) increased prominently when NaNO(3) and KH(2)PO(4) were added in the liquid BG-11culture medium though phosphate had little effect on EPS yield for specific mass of cells. N. flagelliforme cells grew well at 25 degrees C and neutral pH, however, a lower or higher temperature and weak alkaline can promote EPS accumulation. With the increase of the light intensity, the growth rate of N. flagelliforme cells and the EPS accumulation increase accordingly. When N. flagelliforme cells was cultured in BG-11 medium added with 2.5 g L(-1) of NaNO(3) and 0.956 g L(-1) of KH(2)PO(4) at 25 degrees C with 60 micromol photon m(-2) s(-1) of light intensity, 1.05 g L(-1) cell density and 89.9 mg L(-1) EPS yield were achieved respectively. Adopting the optimal conditions established in flask culture, the liquid culture of N. flagelliforme cells in 20-L photobioreactor for 16 days was conducted and a maximum biomass of 1.32 g L(-1) was achieved, which was about 17.6-fold of that in the initial inoculation. The yield of EPS was 228.56 mg L(-1)and about 2.23-fold of that in flask culture. Moreover, the polysaccharides' material was released into the culture medium during cell growth. These released polysaccharides (RPSs), which can be easily recovered from the medium, are favorable for industrial applications.

show abstract

Untitled

Cited by 124 publications

References 20 publications

UV Irradiation and Desiccation Modulate the Three-dimensional Extracellular Matrix of Nostoc commune (Cyanobacteria)

UV Irradiation and Desiccation Modulate the Three-dimensional Extracellular Matrix of Nostoc commune (Cyanobacteria)

Self-assembly of trehalose molecules on a lysozyme surface: the broken glass hypothesis

Accumulation of Exopolysaccharides in Liquid Suspension Culture of Nostoc flagelliforme Cells

Contact Info

Product

Resources

About