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Furmanowa, M.; Olȩdzka, H.; Sykłowska-Baranek, Katarzyna; Jozefowicz, J.; Gieracka, S.

doi:10.1023/a:1005611114628

Cited by 32 publications

(8 citation statements)

References 13 publications

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“…Calli induced from explant material. (e.g., needles, bark, and embryos) include T. baccata, T. brevifolia, T. chinensis, T. cuspidata, T. canadensis, T. wallichiana, T. x media, and T. yunnanesis [20,[26][27][28][29]. To further optimize cell growth, paclitaxel or related taxoid accumulation, and to facilitate ease of extraction from the media, Taxus spp.…”

Section: Callus and Cell Suspension Inductionmentioning

confidence: 99%

“…Abiotic elicitors include the rare earth metal lanthanum, silver nitrate, or vanadyl sulfate [29,39,40]. Biotic elicitors can be grouped into two distinct classes.…”

Section: Elicitation To Increase Paclitaxel Yield In Cell Culturementioning

confidence: 99%

“…Vanadyl sulfate [29] Pathogen related Fusarium spp. [46] Apergillus niger [60] Botrytis cinerea [43] Gliocladium deliqucescens [43] Penicillium minioluteum [43] Fungal cell wall extracts Non-pathogen related…”

Section: Abioticmentioning

confidence: 99%

“…cultures elicited with methyl jasmonate [58,59]. Arachidonic acid and vanadyl sulfate appear to target similar parts of the pathway as salicylic acid, while fungal cell wall material, whether extract or purified compounds, appear to target similar parts of the pathway as methyl jasmonate [29,51,[58][59][60]. The targets of these elicitors have yet to be conclusively identified, though they likely relate to plant signaling pathways.…”

Section: Abioticmentioning

confidence: 99%

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Advancements in the Understanding of Paclitaxel Metabolism in Tissue Culture

Vongpaseuth¹,

Roberts

2007

CPB

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Paclitaxel is a potent chemotherapeutic agent approved in the treatment of a variety of cancers, and under evaluation for the treatment of Alzheimer's and heart disease. Originally isolated from Taxus brevifolia, this highly substituted ring diterpenoid belongs to a family of plant secondary metabolites known as taxoids. Paclitaxel is currently supplied through both a semi-synthetic process and plant cell culture. Taxus spp. cell culture offers the potential to produce large amounts of paclitaxel and related taxoids, although variability in accumulation and low yields represent key limitations. Thus, intense efforts have been put forth towards understanding Taxus spp. metabolism to increase paclitaxel accumulation in cell culture. While elicitation and environmental optimization have provided some success in increasing paclitaxel accumulation in vitro, understanding metabolism of paclitaxel on the molecular level is essential for process optimization. Utilizing direct and indirect molecular techniques, a further understanding of paclitaxel biosynthesis has been gained, though knowledge into other aspects of paclitaxel global metabolism, such as regulation, transport, and degradation is lacking. Taxus spp. cell cultures are highly heterogeneous, displaying significant cell-cell variability in growth and paclitaxel accumulation. Information gathered on culture subpopulations as well as putative transcriptional bottlenecks in paclitaxel biosynthesis, coupled with successful transformation of Taxus spp. will allow for the targeted metabolic engineering of Taxus spp. or other model organisms for paclitaxel accumulation to ensure future supply of this important pharmaceutical.

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Section: Callus and Cell Suspension Inductionmentioning

confidence: 99%

“…Abiotic elicitors include the rare earth metal lanthanum, silver nitrate, or vanadyl sulfate [29,39,40]. Biotic elicitors can be grouped into two distinct classes.…”

Section: Elicitation To Increase Paclitaxel Yield In Cell Culturementioning

confidence: 99%

Section: Abioticmentioning

confidence: 99%

Section: Abioticmentioning

confidence: 99%

See 2 more Smart Citations

Advancements in the Understanding of Paclitaxel Metabolism in Tissue Culture

Vongpaseuth¹,

Roberts

2007

CPB

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“…Nowadays, some methods have been used for the improvement of taxol production in vitro: selection of callus or cell line [6,7], modification of the medium [8] and use of elicitors [9][10][11]. Among them, elicitors are used frequently for promotion of taxol yield, which include methyl jasmonate, abscisic acid, chlorocholine chloride (CCC), salicylic acid, phenylalanine, as well as osmotic regulators such as sucrose, mannitol, sorbitol and polyethylene glycol [8,[12][13][14][15][16][17][18]. CCC is one of the plant growth retardants, which stimulates increase of taxol production in yew logs [9] and mature roots [19] of intact plants.…”

Section: Introductionmentioning

confidence: 99%

Increase of Taxol Production in Taxus globosa Shoot Callus by Chlorocholine Chloride

Barrios¹,

Zhang²,

Sandoval³

et al. 2009

TONPJ

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Calluses from seasonal mature shoots of Taxus globosa Schltdl. were subcultured in new medium containing 1 mg l-1 NAA, 0.5 mg l-1 2, 4-D, 0.025 mg l-1 BA. Calluses growing for 30 and 40 days in a medium added with 0.1-5.0 mg l-1 of chlorocholine chloride (CCC) were harvested for determination of taxol, phenolic compounds, and for polyphenol oxidase (PPO) activity. The results showed that by increasing the CCC concentration, callus growth was decreased, phenolic level was raised up, but taxol content and PPO activity changed differently. The highest taxol production (0.0269 mg g-1 dry weight callus) and PPO activity (4.53 U g-1 fresh weight callus) were achieved at day 40 with a medium containing 0.5 mg l-1 CCC. Possible reason of higher taxol level stimulated by CCC is discussed in the text.

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A new bioreactor for paclitaxel production based on foam separation

Taura

Yamamoto

Hayashi

et al. 2013

Asia-Pacific J Chem Eng

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We report here the development of a new bioreactor (bioreactor with foam separation, BFS) for efficient production and recovery of paclitaxel that circumvents paclitaxel's inhibitory effects on growth through a feedback mechanism. The BFS captures paclitaxel through its hydrophobic interaction with the foam generated by aerated culture medium. The BFS comprises co‐axial 0.65 dm3 outer (150 mm height and 75 mm diameter) and 0.15 dm3 inner vessels (90 mm height and 50 mm diameter) equipped with a ball‐type sparger (5–10 µm pore size, 10 mm diameter) and a wire‐framed draft tube covered by a water‐permeable cellulose bag (150 × 40 mm, 100 µm maximum pore size). The BFS was tested by adding paclitaxel alone to the medium of 0.12 dm3 in the inner vessel and collecting it in the foam that overflowed into the outer vessel. Paclitaxel was separated with first‐order kinetics and was optimally recovered using an aeration rate of 0.05 dm3 min−1 (0.21 vvm). Next, a Taxus cuspidata callus was cultured using the optimal aeration rate. A conventional shaking culture (110 rpm) served as the control using a 0.2 dm3 Erlenmeyer flask (0.12 dm3 medium). Paclitaxel was maintained at less than 0.02 g m−3 in the inner vessel of the BFS. Consequently, there was no feedback inhibition, and the paclitaxel production increased by a factor of five in the BFS compared with the control culture where there was feedback inhibition by the high concentration of paclitaxel. © 2013 Curtin University of Technology and John Wiley & Sons, Ltd.

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Cited by 32 publications

References 13 publications

Advancements in the Understanding of Paclitaxel Metabolism in Tissue Culture

Advancements in the Understanding of Paclitaxel Metabolism in Tissue Culture

Increase of Taxol Production in Taxus globosa Shoot Callus by Chlorocholine Chloride

A new bioreactor for paclitaxel production based on foam separation

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