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Abstract

Materials and Methods Cell line and reagentsHepG2 Cells were obtained from American Type Culture Collection (ATCC, USA) and cultured in a 37 °C incubator with 5% CO2 according to ATCC protocols. Capsaicin was purchased from Enzo Life Science Company. Antibodies of PARP-1, Cyclin-D1, β-actin and all secondary antibodies were purchased from Santa Cruz Bio-technology (Santa Cruz, CA, USA). Growth inhibitionGrowth inhibition was assessed via an MTT assay. Briefly, Hep G2 cells were plated at a density of 30,000 cells/well on 24well plate. After overnight growth, the cells were treated with