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Abstract

Vitamin B17 (Amygdalin) is D-mandelonitrile-bi-glucoside naturally found in fruit seeds. Amygdalin was extracted and purified from the kernels of apricots cultivated in Egypt. Identification of the extract was performed by High-performance liquid chromatography (HPLC), where a major amygdalin peak was obtained at retention time 3.5. Chemical structure of amygdalin was verified using Fourier-transform infrared spectroscopy (FTIR) and Nuclear Magnetic Resonance (1 H-NMR) spectroscopy. Human hepatic (Huh-7) and colorectal (HT-29) cancer cell lines were treated as indicator for cytotoxicity of extracted amygdalin. The IC50 valued >100 µM and 30 µM, respectively.