Untitled

Abstract

Simple, sensitive, and accurate spectrophotometry method one each for estimation of five drugs viz., chloroquine phosphate (CHP), granisetron hydrochloride (GRA), rizatriptan benzoate (RIZ), zoledronic acid (ZOL) and zolmitriptan (ZOT), have been developed. The method depends upon oxidation of each drug by excess N-Bromo succinimide (NS), and subsequent determination of UN reacted NBS by Rhodamine-B dye at λ max 557 nm. Beers law is obeyed between 2-14 µg mL-1 (for CHP); 52-14 µg mL-1 (for GRA); 1-7 µg mL-1 (for RIZ); 1-7 µg mL-1 (for ZOL) & 2-14 µg mL-1 (for ZOT). This method has been applied for the determination of drugs in their pure form as well as in tablet formulations. All the methods have been validated as per ICH guidelines. INTRODUCTION: Chloroquine Phosphate: Chloroquine phosphate (CHP) is an antimalarial drug whose structure is shown in Fig. 1A and chemically knows as 7chloro-4-4-diethylamino-1-methylbutyl amino-quinoline diphosphate. CHP is also used for the treatment of amebiasis, polymyositis, sarcoidosis rheumatoid arthritis, discoid lupus erythematosus, and photosensitive diseases 1. The literature survey reveals that several analytical methods are reported for the quantification of CHP. Among these methods, British Pharmacopoeia (BP) 2 , United States Pharmacopeia (USP) 3 , UVspectro-photometry 4 , HPLC 5 , HPTLC 6 , gas chromatography 7 , polarimetry 8 and electrophoresis 9 are a few mention worth.