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Gireesh, T.; Jayadeep, A.; Rajasekharan, K. N.; Menon, Venugopal P.; Vairamany, M.; Tang, Guangwen; Nair, Padmanabhan P.; Sudhakaran, P. R.

doi:10.1023/a:1010378401621

Cited by 22 publications

(6 citation statements)

References 5 publications

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“…Green, grey-greenish, orange-yellowish and orange bands were found in the TLC plates eluted with both eluent 1 and 2, in agreement with the colors of the main pigments described in Spirulina platensis [25,26]: chlorophyll a, α,β-carotene, zeaxanthin, βcryptoxanthin and other minor carotenoids. Moreover, blue and violet bands were detected in ethanol extracts when eluent 2 was used as mobile phase.…”

Section: Antioxidant Activity Of the Ple Extractssupporting

confidence: 83%

Functional characterization of pressurized liquid extracts of Spirulina platensis

Santoyo

Herrero²,

Señoráns

et al. 2006

Eur Food Res Technol

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Three different parameters (temperature, solvent and extraction time) were studied regarding to pressure liquid extraction of antioxidant and antimicrobial compounds of Spirulina platensis. Two different antioxidant methods, β-carotene bleaching method and DPPH • free radical-scavenging assay, were used to determine the optimal PLE conditions for antioxidant's extraction from Spirulina platensis microalgae. The selected conditions were as follows: extraction temperature equal to 115ºC, extraction time equal to 15 min and ethanol as extracting solvent. The main antioxidant compounds found in this extract were identified as zeaxanthin, a myxoxanthophyll-like compound and very polar phenolic compounds. Moreover, antimicrobial activity of different PLE fractions was tested against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 11775, Candida albicans ATCC 60193 and Aspergillus niger ATCC 16404. Data obtained showed that the hexane and petroleum ether extracts were slightly more active than ethanolic extracts. As for water extracts, none of them were active against the microorganisms tested. Data indicated that both, 115ºC and 170ºCwere the best extraction temperatures in order to optimize the extraction of antimicrobial compounds, whereas 9 min was the optimal extraction time. Besides, Candida albicans was the most sensitive microorganism to all Spirulina PLE extracts.

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Section: Antioxidant Activity Of the Ple Extractssupporting

confidence: 83%

Functional characterization of pressurized liquid extracts of Spirulina platensis

Santoyo

Herrero²,

Señoráns

et al. 2006

Eur Food Res Technol

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“…It is now possible to use advanced technologies to conduct human investigations by using intrinsically labeled spirulina. Spirulina can grow under heavy water to produce an intrinsically labeled product for human absorption and conversion studies [19]. A very recent report used a vitamin A reference dose to determine the vitamin A value of β-carotene from the spirulina grown hydroponically [20].…”

Section: Vitamin a Value Of Spirulinamentioning

confidence: 99%

Vitamin A, Nutrition, and Health Values of Algae: Spirulina, Chlorella, and Dunaliella

Tang

Suter²

2011

J. Pharm. Nutr. Sci.

112

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Spirulina, chlorella, and dunalliella are unicellular algae that are commercially produced worldwide. These algae are concentrated sources of carotenoids (especially provitamin A carotenoids) and other nutrients, such as vitamin B12. Their health benefits as a complementary dietary source for macro and micro nutrients have been studied and confirmed in various populations. The safety of human consuming these algae and products derived from these algae by humans has been widely studied. It is generally concluded that these algae and its products are safe if cultivated properly in a non-contaminated environment, and if consumed in moderation. Abstract: Spirulina, chlorella, and dunalliella are unicellular algae that are commercially produced worldwide. These algae are concentrated sources of carotenoids (especially provitamin A carotenoids) and other nutrients, such as vitamin B12. Their health benefits as a complementary dietary source for macro and micro nutrients have been studied and confirmed in various populations. The safety of human consuming these algae and products derived from these algae by humans has been widely studied. It is generally concluded that these algae and its products are safe if cultivated properly in a non-contaminated environment, and if consumed in moderation.

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“…1a (description of the bands as in Table 1). The main pigments in S. platensis microalga have been described [23,24] as chlorophyll a and different carotenoids such as b-carotene, b-cryptoxanthin, zeaxanthin, echinenone, oscillaxanthin, and myxoxanthophyll. Thus, the orange, yellow, and green bands in analytical-TLC of Fig.…”

Section: Analysis By Tlcmentioning

confidence: 99%

Separation and characterization of antioxidants from Spirulina platensis microalga combining pressurized liquid extraction, TLC, and HPLC‐DAD

Jaime

Mendiola

Herrero

et al. 2005

J of Separation Science

121

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A new procedure has been developed to separate and characterize antioxidant compounds from Spirulina platensis microalga based on the combination of pressurized liquid extraction (PLE) and different chromatographic procedures, such as TLC, at preparative scale, and HPLC with a diode array detector (DAD). Different solvents were tested for PLE extraction of antioxidants from S. platensis microalga. An optimized PLE process using ethanol (generally recognized as safe, GRAS) as extraction solvent has been obtained that provides natural extracts with high yields and good antioxidant properties. TLC analysis of this ethanolic extract obtained at 115 degrees C for 15 min was carried out and the silica layer was stained with a DPPH (diphenyl-pycril-hydrazyl) radical solution to determine the antioxidant activity of different chromatographic bands. Next, these colored bands were collected for their subsequent analysis by HPLC-DAD, revealing that the compounds with the most important antioxidant activity present in Spirulina extracts were carotenoids, as well as phenolic compounds and degradation products of chlorophylls.

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Cited by 22 publications

References 5 publications

Functional characterization of pressurized liquid extracts of Spirulina platensis

Functional characterization of pressurized liquid extracts of Spirulina platensis

Vitamin A, Nutrition, and Health Values of Algae: Spirulina, Chlorella, and Dunaliella

Separation and characterization of antioxidants from Spirulina platensis microalga combining pressurized liquid extraction, TLC, and HPLC‐DAD

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