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Lekakh, I. V.; Bovin, Nicolai V.; Bezyaeva, G. P.; Poverenny, A.M.

doi:10.1023/a:1002887430209

Cited by 10 publications

(6 citation statements)

References 14 publications

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“…We believe that the sharp changes in volume and the presence of ion exchange groups which "wedge" molecules apart are the causes of this high level of degradation of the matrix. It is generally accepted that elution of proteins from anion exchange Sephadexes occurs at NaCl concentrations of no more than 0.3 M. Elution with 0.5 M NaCl is used in the chromatography of immunoglobulins to dissociate immune complexes, which is believed to unmask cryptic antibodies [4]. In these elution conditions, 100 mg/ml of Sephadex WSF are released into the eluate, and this binds to the proteins subjected to chromatography and alters their properties.…”

Section: Resultsmentioning

confidence: 99%

“…Figure 3 shows that mAb, which initially did not interact with DNA, acquired DNAbinding activity after chromatography on QAE-Sephadex. mAb are known to be homogeneous, so the appearance of activity after chromatography cannot be explained by dissocia-tion of complexes resulting in release of cryptic antibodies, as suggested by the authors of [4] as an explanation for chromatographed immunoglobulins. Thus, chromatography on anion exchange Sephadexes does not reveal cryptic antibodies, but produces proteins modified by charged Sephadex WSF.…”

Section: Resultsmentioning

confidence: 99%

“…Desorption conditions as described in [4] lead to degradation of the Sephadex matrix. Complexed with the immunoglobulin molecule, the strongly charged WSF confer cationic properties upon it, this resulting in a cofactor interaction with DNA and many other negatively charged structures.…”

Section: Resultsmentioning

confidence: 99%

“…Water-soluble fragments of DEAE-and QAE-Sephadex were obtained by passing eluting solution consisting of 0.5 M NaCl in 0.01 M phosphate buffer through columns prepared for chromatography pH 7.2 as described in [4]. Given that this models the process of desorption from chromatography columns in conditions in which WSF appeared in the eluate, it follows that the chromatography conditions (eluate, gel) were reproduced.…”

Section: Acidsmentioning

confidence: 99%

“…The properties of these "cryptic" antibodies have been studied over the last 20 years, including their interactions with many negatively charged biological structures (cardiolipin, lipopolysaccharides, phospholipids, DNA, sialic acids, etc. ); they have been suggested to have roles in blood clotting and in controlling humoral immunity [3]; their potential medical uses have been discussed [4]. However, other causes of this interaction cannot be excluded [5 -9].…”

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confidence: 99%

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Immunoglobulin: DNA cofactor interaction

2007

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The reasons for the appearance of DNA-binding activity of immunoglobulins (Ig) after chromatography on anion-exchange Sephadexes were studied. Elution of Ig from these Sephadexes with 0.5 M NaCl was found, using an orcinol method, to be associated with degradation of the matrix, with the appearance of water-soluble fragments (WSF) of Sephadex in the eluate. Degradation of the Sephadex matrix also occurred on washing of unloaded Sephadex. Monoclonal antibodies which did not initially interact with DNA acquired the ability to react with DNA after incubation with Sephadex WSF, as demonstrated using immunoenzyme analysis. It is suggested that the WSF forming on passage of Ig through anion exchange Sephadex columns interact with Ig to generate cations which can react with negatively charged antigens and, particularly DNA. 289 0091-150X/07/4106-0289

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Acidsmentioning

confidence: 99%

mentioning

confidence: 99%

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Immunoglobulin: DNA cofactor interaction

2007

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Natural Antibodies Against Sialoglycans

Шилова

Huflejt

Vuskovic

et al. 2013

Topics in Current Chemistry

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Natural antibodies, part of the innate immunity system, are produced at strictly regulated levels in normal sera without immunization and thus are part of the innate immune system. The best studied natural antibodies are those directed against blood group antigens A and B and xeno-antigens including glycolylneuraminic acid containing Hanganutziu-Deicher (HD) glycolipid. Abnormal levels of anti-glycan antibodies were found in a number of pathologies. In many cases pathological antibodies are known to bind gangliosides. The genesis of anti-glycan antibodies in healthy humans and the reasons for their changes in pathologies are poorly understood. With a growing interest in their diagnostic applications, it is important to determine the carbohydrate structures that are recognized by antibodies present in the circulation of healthy individuals. We tested a large number of healthy donors using a printed glycan array (PGA) in a microchip format. The PGA contained ~300 glycans, representing mostly normal mammalian structures of glycoproteins and glycolipids, and many of the structures presented are biologically relevant sialylated motifs. As revealed by PGA, the sera interacted with at least 70 normal human glycans. With only few exceptions, antibodies recognizing sialosides have not been identified. Moderate levels of antibodies and moderate variability were observed in the case of SiaT n and its glycolyl variant. Unexpectedly, we found minimal antibody titer directed against Neu5Gcα and the trisaccharide Neu5Gcα2-6Galβ1-4GlcNAc, although this form of neuraminic acid does not occur naturally in humans. Antibodies recognizing sialosides in unnatural β-configuration have been detected and confirmed Springer's paradigm that circulating antibodies represent a reaction against bacteria. Gram-negative bacteria contain LPS with βKDN and/or βKDO which are very close analogs of Neu5Ac that are found in β-connected form. Antibodies against the biantennary N-glycan chain, (Neu5Acα2-6Galβ1-4GlcNAcβ1-2Manα)2-3,6-Manβ1-4GlcNAcβ1-4GlcNAc were never observed and similarly we never saw antibodies directed against the SiaLe(a)/SiaLe (x) motifs. Anti-sialoglycan antibodies can be masked with gangliosides: for example, we observe about a five times higher level of anti-GD3 in purified total IgG compared to the same concentration of total Ig in the composition of native serum. For several antibodies we observed anomalous binding in diluted sera, namely, the signals towards sialylated glycans were increased in the PGA if diluted sera were used.

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Naturally Occurring Antibodies Directed Against Carbohydrate Tumor Antigens

Schwartz‐Albiez

2012

Advances in Experimental Medicine and Biology

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Healthy persons carry within their pool of circulating antibodies immunoglobulins preferentially of IgM isotype, which are directed against a variety of tumor-associated antigens. In closer scrutiny of their nature, some of these antibodies could be defined as naturally occurring antibodies due to the germline configuration of the variable immunoglobulin region. The majority of these immunoglobulins recognize carbohydrate antigens which can be classified as oncofetal antigens. Many of these IgM antibodies present in the peripheral blood circulation can bind to tumor cells and of these a minor portion are also able to destroy tumor cells by several mechanisms, as for instance complement-mediated cytolysis or apoptosis. It was postulated that anti-carbohydrate antibodies are part of an anti-tumor immune response, while their presence in the peripheral blood of healthy donors is still waiting for a plausible explanation. It may be that recognition of defined epitopes, including carbohydrate sequences, by naturally occurring antibodies constitutes the humoral arm of an anti-tumor immune response as part of the often postulated tumor surveillance. The cytotoxic capacity of these antibodies inspired several research groups and pharmaceutical companies to design novel strategies of immunoglobulin-based anti-tumor immunotherapy.

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Untitled

Cited by 10 publications

References 14 publications

Immunoglobulin: DNA cofactor interaction

Immunoglobulin: DNA cofactor interaction

Natural Antibodies Against Sialoglycans

Naturally Occurring Antibodies Directed Against Carbohydrate Tumor Antigens

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