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Шакирова, Ф. М.; Безрукова, М. В.; Avalbaev, A.M.; Gimalov, F. R.

doi:10.1023/a:1014805724231

Cited by 16 publications

(3 citation statements)

References 11 publications

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“…Thereafter, 4-day-old seedlings were incubated in 2% sucrose solution supplemented with 0.4 µM EBR for different time intervals. The stock solution of hormone (2 mM EBR in 96% ethanol) was diluted to obtain the effective EBR concentration at 0.4 µM which was selected previously [ 92 ]. For the stress assay, another group of the 3-day-old seedlings was incubated in 2% sucrose solution supplemented with 0.4 µM EBR for a day.…”

Section: Methodsmentioning

confidence: 99%

24-Epibrassinolide Reduces Drought-Induced Oxidative Stress by Modulating the Antioxidant System and Respiration in Wheat Seedlings

Avalbaev,

Fedyaev,

Lubyanova

et al. 2024

Plants

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Brassinosteroids (BRs) represent a group of plant signaling molecules with a steroidal skeleton that play an essential role in plant adaptation to different environmental stresses, including drought. In this work, the effect of pretreatment with 0.4 µM 24-epibrassinolide (EBR) on the oxidant/antioxidant system in 4-day-old wheat seedlings (Triticum aestivum L.) was studied under moderate drought stress simulated by 12% polyethylene glycol 6000 (PEG). It was revealed that EBR-pretreatment had a protective effect on wheat plants as evidenced by the maintenance of their growth rate, as well as the reduction in lipid peroxidation and electrolyte leakage from plant tissues under drought conditions. This effect was likely due to the ability of EBR to reduce the stress-induced accumulation of reactive oxygen species (ROS) and modulate the activity of antioxidant enzymes. Meanwhile, EBR pretreatment enhanced proline accumulation and increased the barrier properties of the cell walls in seedlings by accelerating the lignin deposition. Moreover, the ability of EBR to prevent a drought-caused increase in the intensity of the total dark respiration and the capacity of alternative respiration contributes significantly to the antistress action of this hormone.

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Section: Methodsmentioning

confidence: 99%

24-Epibrassinolide Reduces Drought-Induced Oxidative Stress by Modulating the Antioxidant System and Respiration in Wheat Seedlings

Avalbaev,

Fedyaev,

Lubyanova

et al. 2024

Plants

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“…In particular brassinosteroids are highly effective in decreasing the level of toxic ions damage effects on plant growth [7]. Previously it was found that 24epibrassinolide (EBR) treatment causes the significant WGA gene transcription activation and the increase in WGA content in wheat seedlings as well as the absence in changing in the endogenous ABA concentration [8]. These data, alongside with the previously obtained results on the WGA treatment possibility to enhance cell division of EBR-pretreated seedlings against the background of endogenous lectin deficiency, indicate the interaction of the wheat lectin system and EBR in the regulation of growth processes [9].…”

Section: Introductionmentioning

confidence: 99%

Effect of WGA on 24-Epibrassinolide-Induced Resistance of Wheat Plants and Cell Walls Reinforcement under the Influence of Cadmium Acetate

Безрукова

Lubyanova

Шакирова

2020

The 1st International Electronic Conference on Plant Science

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The generally accepted approach for increasing plant resistance to heavy metals is the treatment by phytohormones of different natures. In particular, brassinosteroids are highly effective in decreasing the level of toxic ions damage effects on plant growth. ABA and wheat germ agglutinin (WGA) contents were measured in the same plant with the help of enzyme immunoassay method. The localization of lignin and suberin on cross sections of wheat roots stained with berberine hemisulfate/toluidine blue was performed. The mitotic index was determined as percentage of mitotic cells of the total number of the cells. It was found that 0.4 μM 24-epibrassinolide (EBR) seed pretreatment under the influence of 1 mM cadmium acetate in the presence of fluridone (Fl) inhibited the stress-induced accumulation of ABA, while the WGA content in plants stayed higher than in stressed EBR-untreated wheat roots. Moreover, EBR-pretreated wheat plants under cadmium stress formed in the root cell walls’ Casparian bands and suberin lamellae, which are the critical locations in the apoplastic barriers of endodermis and exodermis. Pre-sowing EBR-treatment promoted acceleration of Casparian bands and suberin lamellae formation without inhibiting growth processes. We have demonstrated the involvement of wheat lectin in the realization of EBR-induced protective effect on plants under cadmium stress. An important contribution to EBR-induced strengthening the barrier properties of the cell walls of the studied tissues is the ability of EBR to induce ABA-independent accumulation of WGA, which further promotes the increasing lignin and suberin biopolymers deposition in the cell walls of wheat roots.

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“…A dose-response curve with 2 optimum concentrations was obtained for the action of epibrassinolide on the growth of wheat roots (Shakirova et al, 2002). Inhibition of Magnaporthe grisea appressoria by hydrogen peroxide had the main maximum at 10 mM and one or two peaks at 10 -8 to 10 -6 M or less (Aver'yanov et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Effect of some immunomodulators and zeatin on susceptibility of wheat to powdery mildew

Бабоша¹

2019

Turk J Bot

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The effects of zeatin, salicylic acid, and plant oligoadenylates on susceptibility of wheat seedlings to powdery mildew and the shape of dose-response curves for immunomodulation were studied. Salicylic acid and zeatin produced complex dose-responses with upregulation or downregulation of plant susceptibility. Under different experimental conditions, the dose-response curve for zeatin varied in shape from a curve with a maximum to a curve with a minimum of susceptibility to powdery mildew. More complicated curves with a resistance region framed with regions of increased susceptibility were also found. Plant oligoadenylates inhibited the development of powdery mildew at optimal concentrations. The increasing concentrations of salicylic acid and plant oligoadenylates, when combined with zeatin, led to a gradual change in the shape of the zeatin concentration curve. Treatment with salicylic acid and plant oligoadenylates in these experiments may simulate plant responses under biotic and abiotic stresses. Thus, exogenous zeatin was applied to model changes in the phytohormone metabolism. The complex multiphase nature of dose-response is proposed to cause a kind of random-number generator that produces variability in the physiological and immunological status of plants in natural conditions. Variation in susceptibility of individual plants may be crucial for the survival of infected plants and the stabilization of plant-pathogen interactions.

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Cited by 16 publications

References 11 publications

24-Epibrassinolide Reduces Drought-Induced Oxidative Stress by Modulating the Antioxidant System and Respiration in Wheat Seedlings

24-Epibrassinolide Reduces Drought-Induced Oxidative Stress by Modulating the Antioxidant System and Respiration in Wheat Seedlings

Effect of WGA on 24-Epibrassinolide-Induced Resistance of Wheat Plants and Cell Walls Reinforcement under the Influence of Cadmium Acetate

Effect of some immunomodulators and zeatin on susceptibility of wheat to powdery mildew

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