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Abstract

As a part of a screening programme developed to evaluate the antimicrobial activity of basidiomycetes, 317 isolates representing 204 species collected in Spain were screened against a range of human clinical pathogens and laboratory controls. Extracts from 45% of the isolates, representing 109 species, showed antimicrobial activity. Antibacterial activity was more pronounced than antifungal activity. The proportion of extracts from basidiomycetes showing antimicrobial activity was similar to or above that obtained for representative orders of Ascomycetes, such as Pezizales and Xylariales, but lower than that produced by members of the orders Diaporthales, Eurotiales, Hypocreales, Leotiales and Sordariales. Suprageneric taxa (orders and families) did not show pronounced differences in their antimicrobial activities though such differences were observed at the genus level, suggesting that the ability to produce these bioactive compounds is not homogenously distributed amongst the basidiomycetes. Isolates from some species showed large differences in their ability to produce metabolites with antimicrobial activity, possibly reflecting genetic differences at the infraspecific level.

Sponge-Derived Kocuria and Micrococcus spp. as Sources of the New Thiazolyl Peptide Antibiotic Kocurin

“…The frequency of microbiological activities against bacterial human pathogens was considered to be an indicator of their ability to produce anti-infective molecules with potential therapeutic properties [41]. Three out of the 528 extracts that were tested in these bioassays inhibited the growth of MRSA.…”

Fomitopsis betulina (formerly Piptoporus betulinus): the Iceman’s polypore fungus with modern biotechnological potential

“…[zone of inhibition, agar well diffusion assay]Benzene extractsPolyporenic acid (suggested)Utzig and Fertig (1957) Bacillus sp., Rhodococcus equi, S. aureus [zone of inhibition, disk-diffusion method]Chloroform extractsKaraman et al (2009) B. subtilis, Escherichia coli [zone of inhibition, agar well diffusion assay]Dichloromethane extractsKeller et al (2002) Bacillus sp., R. equi, S. aureus, E. coli [zone of inhibition, agar well diffusion assay]Methanol extractsKaraman et al (2009), Keller et al (2002) B. subtilis, Sarcina lutea [zone of inhibition, agar well diffusion assay]Ethanol extractsPolyporenic acid A (suggested)Kandefer-Szerszeń et al (1981) B. subtilis, S. lutea, Brucella sp. [zone of inhibition, agar well diffusion assay]Ether extractsPolyporenic acid (suggested)Kandefer-Szerszeń and Kawecki (1974), Utzig and Fertig (1957) B. subtilis, Enterococcus faecalis, E. coli, S. aureus [zone of inhibition, agar well diffusion assay, NCCLS-method]Piptamine isolated from submerged culture of F. betulina Schlegel et al (2000) B. subtilis, E. coli, S. aureus [zone of inhibition assay]Mycelium, culture liquidKrupodorova et al (2016) B. subtilis, S. aureus [zone of inhibition assay]3β-acetoxy-16α hydroxyl-24-oxo-5α-lanosta-8-ene-21-oic acidAlresly et al (2016) E. faecalis [zone of inhibition assay]Alkali extractVunduk et al (2015)FungicidalInhibition of fungal growth Saccharomyces cerevisiae, Aspergillus fumigatus , [zone of inhibition, agar well diffusion assay]ExtractsSuay et al (2000) …”

Prescreening bacterial colonies for bioactive molecules with Janus plates, a SBS standard double-faced microbial culturing system

“…Optical density (OD 660 ) of the overnight inoculum was measured and adjusted to 0.4, and 30 ml of the inoculum were added to 1 l of YNBD agar (6.7 g yeast nitrogen base, 1 g glucose, 15 g agar, distilled H 2 O 1 l) at 40 °C (Suay et al 2000; Bills et al 2008), which was poured onto the assay layer of the Janus plates.…”

“…The OD 600 was adjusted to 0.4 in 30 ml of 1.5 % LB agar at 40 °C (Suay et al 2000) and subsequently poured to the assay layer of Janus plates.…”