Untitled

Chen, Zhaolie; Lütkemeyer, Dirk; Iding, Kai; Lehmann, Jürgen

doi:10.1023/a:1010373527375

Cited by 9 publications

(4 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microcarriers have been developed to grow anchorage-dependent cell lines, such as CHO cells in bioreactors [8,9] . For the mass production of recombinant proteins, it is proposed that high density culture of mammalian cells should be developed [7] . Although microcarriers have micropores and active surfaces for cell attachment, it seems that the 3D nanofiber mats, with high surface-tovolume ratios, are more suitable substitutes for microcarriers in bioreactors.…”

Section: Discussionmentioning

confidence: 99%

“…The maximum amount of rhFVIII was also obtained with the PCL-PEG polyblend nanofiber mat (80:20)14 % , giving rise to a maximum cell density. The rhFVIII is constitutively produced by the rCHO cell line; therefore, it is not surprising that higher concentrations of recombinant protein are produced as the cell density increases [7] .…”

Section: Cell Viability and Functionmentioning

confidence: 99%

“…Efforts in the area of production technology are continuously being made to increase volumetric productivity, batch consistency, and homogeneity of product quality at competitive costs. Moreover, high cell density cultures have been improved to increase volumetric productivity, because the overall productivity of recombinant proteins is proportional to both the final cell density and specific productivity (the amount of product formed per unit cell mass) [7] .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of PCL-PEG nanofibrous mats as alternative carriers for recombinant Chinese hamster ovary cells

Shakibaie¹,

Rahbar

Tabandeh

et al. 2012

Journal of Polymer Engineering

View full text Add to dashboard Cite

In this study, six polyblended nanofiber mats, composed of poly ε-caprolactone (PCL)-polyethylene glycol (PEG) mixed at different proportions in an electrospinning solution were fabricated, and then utilized alongside commercial polyester microcarriers for the mass cultivation of recombinant Chinese hamster ovary (rCHO) cells. The SEM micrographs showed that the rCHO cells were attached to the nanofiber mats with significant differences in their affinities, which can be related to the chemical structure and hydrophilicity of the nanofiber mats. The cell densities of the rCHO cells grown on the polyblended PCL-PEG nanofiber mat (80:20)14 % were much higher when compared with the commercial polyester microcarrier. Moreover, recombinant protein production increased by approximately 20 % when cells were grown on the PCL-PEG nanofiber mat (80:20)14 % . The results of this research indicated a potential for the replacement of the commercial polyester microcarrier with the PCL-PEG polyblended nanofiber mats, in order to achieve mass cultivation of recombinant animal cells in industrial bioreactors.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Cell Viability and Functionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development of PCL-PEG nanofibrous mats as alternative carriers for recombinant Chinese hamster ovary cells

Shakibaie¹,

Rahbar

Tabandeh

et al. 2012

Journal of Polymer Engineering

View full text Add to dashboard Cite

show abstract

“…Animal cell culture using bioreactors now plays an important role in the production of biologics [4][5][6]. The biological products produced by animal cell culture include diagnostic monoclonal antibodies, protein pharmaceuticals, and viral vaccines [7][8][9][10][11] Bioreactors have also been used to produce quantities of cells in short supply naturally, such as stem cells.…”

Section: Introductionmentioning

confidence: 99%

The Bioreactor: A Powerful Tool for Large-Scale Culture of Animal Cells

Dian-liang¹,

Liu²,

Han³

et al. 2005

CPB

View full text Add to dashboard Cite

Bioreactors play a key role in the field of biologics, where they are used for the production of recombinant therapeutic proteins by large-scale cultivation of animal cells. There are several types of bioreactors, including stirred-tank, airlift, hollow-fiber, and Rotary Cell Culture System (RCCS) designs. The stirred-tank bioreactor is one of the most commonly used types, and is used both for industrial applications and laboratory research. The RCCS, invented by NASA, is increasingly used in the area of tissue engineering for medical purposes. Important improvements have been made in the design of traditional bioreactors, and new types of bioreactor are also being developed such as Couette-Taylor bioreactor, multifunctional-membrane bioreactor, and shaking bioreactor. Work is also progressing on techniques to improve the performance of bioreactors, including perfusion culture, the use of microcarriers, and methods of suppressing apoptosis and of monitoring cell growth in real time. Given the demand for the production by animal cells for use in the growing number of clinical applications, further advances in bioreactor technology can be expected during the next few years. Two main goals will be pursued: firstly, to increase output by high density cultivation of animal cells to produce high value protein pharmaceutics or viral vectors for clinical gene therapy; and secondly, to create a three-dimension space similar to that of an in vivo environment to regenerate tissue or organ and to reproduce valuable cells that are hard to culture in the traditional culture system.

show abstract

High yields of monomeric recombinant β‐interferon from macroporous microcarrier cultures under hypothermic conditions

Tharmalingam

Sunley

Butler

2008

Biotechnology Progress

View full text Add to dashboard Cite

Macroporous microcarriers such as Cytopore entrap mammalian cells in a mesh network allowing growth to high cell concentrations in a protected environment within a stirred culture. Chinese hamster ovary (CHO) cells producing recombinant human b-interferon (IFNb) and grown in Cytopore microcarriers showed a 2.6-to 2.8-fold increase in the volumetric product titer compared with cells grown in an equivalent suspension culture. In an attempt to maximize production of IFN-b, microcarrier cultures were subjected to a low temperature regime. Low temperature culture conditions (328C) have been shown previously to enhance cell specific productivity in suspension cultures although at reduced cell growth rates. These conditions can be optimized by a timely shift from physiological to hypothermic conditions during the culture run to maximize volumetric protein production. In the case of IFN-b production the lower temperature has the added advantage of stabilizing the product and reducing intramolecular aggregation. Using a biphasic temperature-shift regime from 37 to 328C the volumetric production of IFN-b was enhanced to 4.2-fold compared with a single temperature suspension culture in a controlled bench-top bioreactor. Furthermore, the degree of intramolecular aggregation of IFN-b was reduced significantly (59%) compared with control cultures, largely due to the lower temperature but also partially due to the presence of microcarriers. These results indicate that the hypothermic conditions in a Cytopore culture had a combined and possibly synergistic effect of increasing volumetric production of the recombinant protein.

show abstract

Untitled

Cited by 9 publications

References 8 publications

Development of PCL-PEG nanofibrous mats as alternative carriers for recombinant Chinese hamster ovary cells

Development of PCL-PEG nanofibrous mats as alternative carriers for recombinant Chinese hamster ovary cells

The Bioreactor: A Powerful Tool for Large-Scale Culture of Animal Cells

High yields of monomeric recombinant β‐interferon from macroporous microcarrier cultures under hypothermic conditions

Contact Info

Product

Resources

About