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Ouabain exerts neurotoxic action and activates the population of NMDA receptors. Herein the effect of ouabain on the expression of NMDA subunits was evaluated. Adult Wistar rats were administered intracerebroventricularly with 0.1, 10 and 100 nmol ouabain or saline solution (control). Two days later, membranes of cerebral cortex and hippocampus were isolated. Western blots with antibodies for the NMDA receptor subunits: NR1; NR2A; NR2B; NR2C and NR2D were carried out. In cerebral cortex, NR2D subunit increased 30% with 10 nmol ouabain dose. With 100 nmol ouabain, NR1 and NR2D subunits enhanced 40 and 20%, respectively. In hippocampus, with the dose of 0.1 nmol ouabain, NR1 subunit enhanced roughly 50% whereas NR2B subunit decreased 30%. After administration of 10 nmol ouabain dose, NR2A, NR2B and NR2C subunits decreased 40, 50 and 30%, respectively. With the dose of 100 nmol of ouabain, NR1, NR2A and NR2B subunits diminished 10-20%. It is concluded that ouabain administration led to a differential regulation in the expression of NMDA subunits. These results may be correlated with the modulatory action of ouabain on NMDA receptor.

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“…, K ? -ATPase regulators [39][40][41]. Previous work from this laboratory led to the isolation of an endogenous brain Na ?…”

Section: Discussionmentioning

confidence: 99%

Intracerebroventricular Administration of Ouabain to Rats Changes the Expression of NMDA Receptor Subunits in Cerebral Cortex and Hippocampus

Bersier

Arnaiz

2009

Neurochem Res

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Na+, K+-ATPase in the Brain: Structure and Function

Arnaiz¹

2007

Handbook of Neurochemistry and Molecular Neurobiology

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Inhibition of ATPases Enzyme Activities on Brain Disturbing Normal Oestrous Cycle

Venugopal

Ramakrishna

2005

Neurochem Res

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Wistar rats treated with alpha-methyl-DL-p-tyrosine methylester showed significant level of inhibition in the activity of Na+, K+ -ATPase, Mg2+ -ATPase and Ca2+ -ATPase enzymes in different regions of the brain. The enzyme activity was assayed in cerebral hemispheres, hypothalamus, thalamus, hippocampus, amygdala and septum at proestrous (12 h), estrous (25 h), metestrous (38 h) and diestrous periods (92 h) of the rat. The Na+, K+ -ATPase activity was significantly inhibited in most of the brain regions after treated with alpha-methyl-DL-p-tyrosine methylester (MPT) and this indicated that MPT affected the active transport system and nerve impulse transmission. Mg2+ -ATPase and Ca2+ -ATPase was also significantly (P < 0.001) reduced in different regions of the brain. The results revealed that MPT affected active transport system and nerve impulse transmission by inhibiting Na+, K+ -ATPase and Ca2+ -ATPase. It has induced energy crisis by inhibiting Mg2+ -ATPase and all these cumulative effects of MPT have adversely affected the female Wistar rats. These effects have been manifested in the form of aberrations in the behavior of MPT treated female rats, which have shown their inability to perform their normal sexual activity.

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Cited by 21 publications

References 97 publications

Intracerebroventricular Administration of Ouabain to Rats Changes the Expression of NMDA Receptor Subunits in Cerebral Cortex and Hippocampus

Intracerebroventricular Administration of Ouabain to Rats Changes the Expression of NMDA Receptor Subunits in Cerebral Cortex and Hippocampus

Na+, K+-ATPase in the Brain: Structure and Function

Inhibition of ATPases Enzyme Activities on Brain Disturbing Normal Oestrous Cycle

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