A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system

Andrews, Simon C.; Berks, Ben C.; McClay, Joseph L.; Ambler, Andrew; Quail, Michael A.; Golby, Paul; Guest, John R.

doi:10.1099/00221287-143-11-3633

Cited by 248 publications

(245 citation statements)

References 86 publications

(134 reference statements)

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“…Therefore, the block in CO 2 reduction lies between CO 2 and methylene-H 4 SPT. Finally, acetate was not converted to CH 4 and CO 2 at detectable levels by cell suspensions of either wild-type or Ech1 grown on methanol. Thus, we measured the oxidation of CO to CO 2 and H 2 , which represents the oxidative half of acetoclastic methanogenesis.…”

Section: Construction and Verification Of An M Barkeri ⌬Echmentioning

confidence: 90%

“…Recently, a novel family of multisubunit membrane-bound [NiFe] hydrogenases, which are closely related to proton-pumping NADH:quinone oxidoreductases (complex I in electron transport chain of mitochondria), has been recognized (1,2). This family includes hydrogenases 3 and 4 from Escherichia coli (3,4), CO-induced hydrogenase from Rhodospirillum rubrum (5,6), Eha and Ehb from Methanothermobacter species (1), and Ech hydrogenase (Ech) from Methanosarcina barkeri Fusaro (7). Various members of this family have been proposed to function in energy-conserving electron transport, reverse electron transport, or both.…”

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confidence: 99%

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Genetic analysis of the archaeon Methanosarcina barkeri Fusaro reveals a central role for Ech hydrogenase and ferredoxin in methanogenesis and carbon fixation

Meuer

Kuettner

Zhang

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

166

174

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(ii) Under autotrophic growth conditions, the enzyme catalyzes the energetically unfavorable reduction of ferredoxin by H 2, most probably driven by reversed electron transport, and the reduced ferredoxin thus generated functions as low potential electron donor for the synthesis of pyruvate in an anabolic pathway. (iii) Reduced ferredoxin in addition provides the reducing equivalents for the first step of methanogenesis from H 2͞CO2, the reduction of CO2 to formylmethanofuran. Thus, in vivo genetic analysis has led to the identification of the electron donor of this key initial step of methanogenesis.

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Section: Construction and Verification Of An M Barkeri ⌬Echmentioning

confidence: 90%

mentioning

confidence: 99%

Genetic analysis of the archaeon Methanosarcina barkeri Fusaro reveals a central role for Ech hydrogenase and ferredoxin in methanogenesis and carbon fixation

Meuer

Kuettner

Zhang

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

166

174

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“…Other highly-repressed genes include hyfD (4.3-fold), which encodes a hydrogenase membrane subunit (Andrews et al, 1997), frlA (3.0-fold), which encodes a fructoselysine transporter (Wiame et al, 2002) and yieL (7.0-fold), which encodes a putative xylanase; all three loci are repressed in LB glu biofilms by the AI-2 exporter TqsA (Herzberg et al, 2006). Finding differential expression of these biofilm-related genes on deletion of yncC supports Genes studied by a gel shift experiment with YncC.…”

Section: Genes Regulated By Yncc In Biofilmsmentioning

confidence: 99%

Escherichia coli transcription factor YncC (McbR) regulates colanic acid and biofilm formation by repressing expression of periplasmic protein YbiM (McbA)

2008

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Quorum-sensing signal autoinducer 2 (AI-2) stimulates Escherichia coli biofilm formation through the motility regulator MqsR that induces expression of the putative transcription factor encoded by yncC. Here, we show that YncC increases biofilm formation by repressing overproduction of the exopolysaccharide identified as colanic acid (corroborated by decreasing mucoidy and increased sensitivity to bacteriophage P1 infection). Differential gene expression and gel shift assays demonstrated that YncC is a repressor of the predicted periplasmic protein-encoding gene, ybiM, which was corroborated by the isogenic yncC ybiM double mutation that repressed the yncC phenotypes (biofilm formation, colanic acid overproduction, mucoidy and bacteriophage resistance). Through nickel-enrichment DNA microarrays and additional gel shift assays, we found that the putative transcription factor B3023 (directly upstream of mqsR) binds the yncC promoter. Overexpressing MqsR, AI-2 import regulators LsrR/LsrK and AI-2 exporter TqsA induced yncC transcription, whereas the AI-2 synthase LuxS and B3023 repressed yncC. MqsR has a toxic effect on E. coli bacterial growth, which is partially reduced by the b3023 mutation. Therefore, AI-2 quorum-sensing control of biofilm formation is mediated through regulator MqsR that induces expression of the transcription factor YncC. YncC inhibits the expression of periplasmic YbiM, which prevents overproduction of colanic acid (excess colanic acid causes mucoidy) and prevents YbiM from inhibiting biofilm formation.

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“…More recently, the existence of a fourth enzyme has been postulated based on a genetic analysis of the E. coli chromosome (Andrews et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli

et al. 1999

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Synthesis of the [NiFe] hydrogenases 1 and 2 of Escherichia coli is induced inresponse to anaerobiosis and is repressed when nitrate is present in the growth medium. The hydrogenase 1 and hydrogenase 2 enzymes are encoded by the polycistronic hyaABCDEF and hybOABCDEFG operons, respectively. Primer extension analysis was used to determine the initiation site of transcription of both operons. This permitted the construction of single-copy lacZ operon fusions, which were used to examine the transcriptional regulation of the two operons. Expression of both was induced by anaerobiosis and repressed by nitrate, which is in complete accord with earlier biochemical studies. Anaerobic induction of the hyb operon was only partially dependent on the FNR protein and, surprisingly, was enhanced by an arcA mutation. This latter result indicated that ArcA suppresses anaerobic hyb expression and that a further factor, which remains to be identified, is involved in controlling anaerobic induction of operon expression. Nitrate repression of hyb expression was mediated by the NarL/NarX and NarP/NarQ two-component regulatory systems. Remarkably, a narP mutant lacked anaerobic induction of hyb expression, even in the absence of added nitrate. Anaerobic induction of hya expression was dependent on the ArcA and AppY regulators, which confirms earlier observations by other authors. Nitrate repression of the hya operon was mediated by both NarL and NarP. Taken together, these data indicate that although the hya and hyb operons share common regulators, there are important differences in the control of expression of the individual operons.

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A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system

Cited by 248 publications

References 86 publications

Genetic analysis of the archaeon Methanosarcina barkeri Fusaro reveals a central role for Ech hydrogenase and ferredoxin in methanogenesis and carbon fixation

Genetic analysis of the archaeon Methanosarcina barkeri Fusaro reveals a central role for Ech hydrogenase and ferredoxin in methanogenesis and carbon fixation

Escherichia coli transcription factor YncC (McbR) regulates colanic acid and biofilm formation by repressing expression of periplasmic protein YbiM (McbA)

Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli

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