A 1H NMR spectroscopic method for the quantification of propenylbenzenes in the essential oils: Evaluation of key odorants, antioxidants and post-harvest drying techniques for Piper betle L.

Kemprai, Phirose; Mahanta, Bhaskar Protim; Bora, Pranjit Kumar; Das, Deep Jyoti; Boruah, Jyoti Lakshmi Hati; Saikia, Siddhartha Proteem; Haldar, Saikat

doi:10.1016/j.foodchem.2020.127278

Cited by 22 publications

(19 citation statements)

References 32 publications

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“…As shown in Figure 1c, acetic acid has strong positive correlation with variables in the aromatic region of the 1 H-NMR spectra (δ 6.80 and 6.84) and variables that represent acetate protons of phenylpropene acetate esters (δ 2.04 and 2.08) [19]. In the biplot, these four variables are close to each other, thus providing support that they may belong to acetate esters of phenylpropenes, which are secondary metabolites commonly reported as constituents of P. betle leaf essential oil [20]. Meanwhile, the PC scores of "sirih merah" are heavily influenced by slightly different variables in the aromatic region (δ 6.28, 6.32, and 6.36, Figure S3), showing an abundance of a group of phenolic metabolites with different substituents on the benzene ring [21].…”

Section: Principal Components Analysis Of 1 H-nmr Spectra Of Three "Sirih" Variantsmentioning

confidence: 88%

“…Further examination of other top candidates in MassHunter's list of hits located chavibetol, with a match score of 85 and LRI that matched literature data [46]. In the literature, either eugenol or its isomer chavibetol were reported as major constituents and chemical markers of P. betle leaf extracts [19,20,[47][48][49][50][51][52][53][54]. Apparently, some studies that reported eugenol as the major constituent of P. betle leaf extracts based the identification solely on high match score or comparison with literature data without application of any other filtering method [12,19,49,54].…”

Section: Leaf Essential Oil Profiles Of P Betle Variants and P Rubro-venosummentioning

confidence: 99%

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Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian Piper betle L. Variants

Osman

Lee

Sarbini

et al. 2021

Plants

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The differences in pungency of “sirih” imply the probable occurrence of several variants of Piper betle L. in Malaysia. However, the metabolite profiles underlying the pungency of the different variants remain a subject of further research. The differences in metabolite profiles of selected Malaysian P. betle variants were thus investigated; specifically, the leaf aqueous methanolic extracts and essential oils were analyzed via 1H-NMR and GC-MS metabolomics, respectively. Principal component analysis (PCA) of the 1H-NMR spectral data showed quantitative differences in the metabolite profiles of “sirih melayu” and “sirih india” and revealed an ambiguous group of samples with low acetic acid content, which was identified as Piper rubro-venosum hort. ex Rodigas based on DNA sequences of the internal transcribed spacer 2 (ITS2) region. The finding was supported by PCA of two GC-MS datasets of P. betle samples obtained from several states in Peninsular Malaysia, which displayed clustering of the samples into “sirih melayu” and “sirih india” groups. Higher abundance of chavicol acetate was consistently found to be characteristic of “sirih melayu”. The present research has provided preliminary evidence supporting the notion of occurrence of two P. betle variants in Malaysia based on chemical profiles, which may be related to the different genders of P. betle.

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Section: Principal Components Analysis Of 1 H-nmr Spectra Of Three "Sirih" Variantsmentioning

confidence: 88%

Section: Leaf Essential Oil Profiles Of P Betle Variants and P Rubro-venosummentioning

confidence: 99%

Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian Piper betle L. Variants

Osman

Lee

Sarbini

et al. 2021

Plants

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“…Six batches were subjected to different drying methods, including air-drying, oven-drying at 40 and 60 °C, sun-drying, microwave-drying, and freeze-drying. The drying conditions and the durations were maintained as per previous reports with slight modifications ensuring that the moisture content in the sample was reduced below 20%. ,− For air-drying, the rhizome slices were kept under shade at room temperature (25–30 °C) for 3 days. Sun-drying was also performed for 3 days under direct sunlight.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Postharvest Drying, Key Odorants, and Phytotoxins in Plai (Zingiber montanum) Essential Oil

Bora

Saikia

Kemprai

et al. 2021

J. Agric. Food Chem.

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Plai or cassumunar ginger (Zingiber montanum), mainly distributed in tropical Asia, is an essential oil-bearing rhizomatous crop belonging to the Zingiberaceae family. Rhizomes and essential oil of this herb are used in culinary as flavoring agents, traditional medicines, and aromatherapy. In this study, the effect of different postharvest drying methods (air-, oven-drying at 40 and 60 °C, sun-, microwave-, and freeze-drying) of its sliced rhizome on the essential oil yield, composition, and sensory quality was investigated. The major key odorants and phytotoxins in its essential oil were identified for the first time through sensory- or bioassay-guided fractionation. Although the drying methods did not alter the oil composition significantly, oven-drying at 40 °C and freeze-drying produced the highest oil yield (81.0% of fresh rhizome) while maintaining the sensory quality. 4-Terpineol was found to be the majorly abundant key odorant in its oil through detailed sensory analysis. This oxygenated monoterpene was also demonstrated to be the major phytotoxin negatively affecting seed germination and shoot and root growth of wheatgrass seeds with IC50 values of 0.67, 0.10, and 0.17 mM, respectively. The current study is beneficial for further value addition of this crop in food industries and the agricultural sector.

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“…In this context, other analytical techniques were proposed for this purpose, such as infrared (IR) and nuclear magnetic resonance (NMR) spectroscopy [ 12 , 13 , 14 , 15 ]. While IR spectroscopy lacks in identifying molecules sharing a similar structure due to signal overlapping, in the case of NMR different compounds can be discriminated.…”

Section: Introductionmentioning

confidence: 99%

A Novel qNMR Application for the Quantification of Vegetable Oils Used as Adulterants in Essential Oils

et al. 2021

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Essential oils (EOs) are more and more frequently adulterated due to their wide usage and large profit, for this reason accurate and precise authentication techniques are essential. This work aims at the application of qNMR as a versatile tool for the quantification of vegetable oils potentially usable as adulterants or diluents in EOs. This approach is based on the quantification of both 1H and 13C glycerol backbone signals, which are actually present in each vegetable oil containing triglycerides. For the validation, binary mixtures of rosemary EO and corn oil (0.8–50%) were prepared. To verify the general feasibility of this technique, other different mixtures including lavender, citronella, orange and peanut, almond, sunflower, and soy seed oils were analyzed. The results showed that the efficacy of this approach does not depend on the specific combination of EO and vegetable oil, ensuring its versatility. The method was able to determine the adulterant, with a mean accuracy of 91.81 and 89.77% for calculations made on 1H and 13C spectra, respectively. The high precision and accuracy here observed, make 1H-qNMR competitive with other well-established techniques. Considering the current importance of quality control of EOs to avoid fraudulent practices, this work can be considered pioneering and promising.

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A 1H NMR spectroscopic method for the quantification of propenylbenzenes in the essential oils: Evaluation of key odorants, antioxidants and post-harvest drying techniques for Piper betle L.

Cited by 22 publications

References 32 publications

Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian Piper betle L. Variants

Metabolomics-Driven Discovery of an Introduced Species and Two Malaysian Piper betle L. Variants

Evaluation of Postharvest Drying, Key Odorants, and Phytotoxins in Plai (Zingiber montanum) Essential Oil

A Novel qNMR Application for the Quantification of Vegetable Oils Used as Adulterants in Essential Oils

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