A 3-YEAR FOLLOW-UP OF A BRAZILIAN AIDS PATIENT WITH PROTRACTED DIARRHEA CAUSED BY Enterocytozoon bieneusi

Brasil, Patrícia; Paiva, Daurita Darci de; Lima, Dirce Bonfim de; Silva, Edson Jurado da; Peralta, José Mauro; Silva, Alexandre J. da; Sodré, Fernando Campos; Villela, Erika Veríssimo; Moura, Hércules

doi:10.1590/s0036-46651998000400002

Cited by 8 publications

(5 citation statements)

References 23 publications

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“…Complete resolution of diarrhea, as observed with the use of antiretroviral combinations with protease inhibitors, could not be evaluated since these drugs were not available during the current study 5,6,10,12,18,29 .…”

Section: Discussionmentioning

confidence: 99%

Clinical and diagnostic aspects of intestinal microsporidiosis in HIV-infected patients with chronic diarrhea in Rio de Janeiro, Brazil

Brasil

Lima

Paiva

et al. 2000

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe objectives of this study were to determine both the prevalence of microsporidial intestinal infection and the clinical outcome of the disease in a cohort of 40 HIV-infected patients presenting with chronic diarrhea in Rio de Janeiro, Brazil. Each patient, after clinical evaluation, had stools and intestinal fragments examined for viral, bacterial and parasitic pathogens. Microsporidia were found in 11 patients (27.5%) either in stools or in duodenal or ileal biopsies. Microsporidial spores were found more frequently in stools than in biopsy fragments. Samples examined using transmission electron microscopy (n=3) or polymerase chain reaction (n=6) confirmed Enterocytozoon bieneusi as the causative agent. Microsporidia were the only potential enteric pathogens found in 5 of the 11 patients. Other pathogens were also detected in the intestinal tract of 21 patients, but diarrhea remained unexplained in 8. We concluded that microsporidial infection is frequently found in HIV infected persons in Rio de Janeiro, and it seems to be a marker of advanced stage of AIDS.

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Section: Discussionmentioning

confidence: 99%

Clinical and diagnostic aspects of intestinal microsporidiosis in HIV-infected patients with chronic diarrhea in Rio de Janeiro, Brazil

Brasil

Lima

Paiva

et al. 2000

Rev. Inst. Med. trop. S. Paulo

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“…This is transmitted through the fecal-oral route and the mature Microsporidia spores are gram-positive 9,10,11 . Microsporidia are usually frequent in HIV patients with CD4 lymphocyte counts of less than 50 cells per mm 3 . They are generally identified in immunodeficient patients but have also been found in immunocompetent patients 9,10 .…”

Section: Introductionmentioning

confidence: 99%

Microsporidiosis in a Brazilian University Hospital: case report

Gonçalves

Uemura

Orban

et al. 2006

Rev. Inst. Med. trop. S. Paulo

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This is the report on a patient with chronic diarrhea caused by microsporidia. He is married, infected with HIV and has low CD4 cell count. The diagnosis was established through stool parasite search using concentration methods and Gram-chromotrope staining technique. Ileum biopsy was also performed in this case. The etiological diagnosis may be established in a clinical laboratory, by chromotrope staining technique in routine microscopic examination of stool specimens.

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“…In Brazil, nine studies have used molecular methods to detect this pathogen in HIV-infected patients presenting chronic diarrhoea, and animals including cattle, pigs, chickens, coatis, pigeons, and exotic birds, which highlights the potential risk of transmission to healthy humans (Brasil et al 1998, 2000, Feng et al 2011, Lallo et al 2012a, b, Fiuza et al 2015, 2016a, b, Cunha et al 2016). However, no information regarding E. bieneusi presence in environmental samples in Brazil is available.…”

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confidence: 99%

Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil

Yamashiro

Fiúza

Teixeira

et al. 2017

Mem. Inst. Oswaldo Cruz

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BACKGROUND Enterocytozoon bieneusi are the most common microsporidia associated with different clinical manifestations such as diarrhoea, respiratory tract inflammation and acalculous cholecystitis, especially in immunocompromised patients. Infection usually occurs by ingestion of food and water contaminated with spores, but can also result from direct contact with spores through broken skin, eye lesions, and sexual transmission, depending on the microsporidian species. Although there are reports of E. bieneusi found in humans and animals in Brazil, there are no published studies of environmental samples examined by molecular methods.OBJECTIVES The purpose of this study was to verify the presence of E. bieneusi in raw sewage and treated effluent from a combined system by molecular methods.METHODS Raw sewage and treated effluent samples collected from a combined system were analysed for the presence of E. bieneusi using the internal transcriber spacer (ITS) region of E. bieneusi by nested polymerase chain reaction.FINDINGS The analysis revealed E. bieneusi presence and a novel genotype (EbRB) in one raw sewage sample and one treated effluent.MAIN CONCLUSIONS The presence of E. bieneusi in final effluent indicates that the combined system may not remove microsporidian spores. This study is the first report of E. bieneusi in environmental samples in Brazil.

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A 3-YEAR FOLLOW-UP OF A BRAZILIAN AIDS PATIENT WITH PROTRACTED DIARRHEA CAUSED BY Enterocytozoon bieneusi

Cited by 8 publications

References 23 publications

Clinical and diagnostic aspects of intestinal microsporidiosis in HIV-infected patients with chronic diarrhea in Rio de Janeiro, Brazil

Clinical and diagnostic aspects of intestinal microsporidiosis in HIV-infected patients with chronic diarrhea in Rio de Janeiro, Brazil

Microsporidiosis in a Brazilian University Hospital: case report

Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil

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