A 31P and 1H-NMR investigation in vitro of normal and abnormal human liver

Bell, Jimmy D.; Cox, I. Jane; Sargentoni, J; Peden, Carol J.; Menon, DK; Foster, Christopher S.; Watanapa, P.; Iles, Richard A.; Urenjak, Jutta

doi:10.1016/0925-4439(93)90124-j

Cited by 84 publications

(88 citation statements)

References 31 publications

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“…Unlike spectra from other areas of the body such as muscle and brain, spectra acquired from the liver do not contain signal from phosphocreatine, since hepatocytes have a dual blood supply from the systemic and portal systems and do not express cytoplasmic creatine kinase, the enzyme responsible for the production of phosphocreatine. 42 This opens up the possibility of using the gene for cytoplasmic creatine kinase as an MR visible marker of gene transfection in gene therapy of the liver. Successful delivery of this marker Gene Therapy gene would allow detection of a phosphocreatine signal that was not previously evident.…”

Section: Mr Spectroscopy and Gene Expressionmentioning

confidence: 99%

Assessing gene expression in vivo: magnetic resonance imaging and spectroscopy

Bell

Taylor‐Robinson

2000

Gene Ther

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Section: Mr Spectroscopy and Gene Expressionmentioning

confidence: 99%

Assessing gene expression in vivo: magnetic resonance imaging and spectroscopy

Bell

Taylor‐Robinson

2000

Gene Ther

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“…Phospholipid cell membrane degradation products and endoplasmic reticulum are the main components of the phosphodiester (PDE) peak. 47 Information on tissue bioenergetics can be obtained from the inorganic phosphate (P i ) and the three nucleotide triphosphate (NTP) resonances. The NTP resonances are not only composed of ATP, but uridine triphosphate, guanosine triphosphate, inosine triphosphate, and cytosine triphosphate also contribute in part.…”

Section: Mrs and Isolated Donor Liver Viabilitymentioning

confidence: 99%

Liver transplantation: Current and potential applications of magnetic resonance spectroscopy

Davidson

1997

Liver Transplantation

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Magnetic resonance spectroscopy (MRS) allows the noninvasive measurement of whole organ metabolism due to the presence of the MRsensitive nucleus phosphorus 31 in adenosine triphosphate (ATP), its precursors, and breakdown products. In small animal liver transplant studies it has been used to analyze the metabolic effects of cold and warm ischemia, hypothermic reperfusion, and the relative efficacy of different organ preservation solutions. In recent large animal studies MRS has been developed to provide continuous dynamic information on ATP metabolism during graft reperfusion and the bioenergetic consequences of altering preservation solutions. These basic experimental data need to be critically evaluated in human liver transplantation. Encouraging preliminary data on many possible clinical applications have already been obtained, such as the assessment of human donor liver viability and posttransplant graft function. At present, the cost and technically demanding nature of MRS may restrict its application to research units.

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“…A significant increase in phosphoethanolamine (PER), phosphocholine (PC), taurine and lactate has been reported, with a reduction in GPE glycerophosphorylethanolamine (GPE) and glycerophosphorylcholine (GPC) in tumours, compared to normal liver controls [24] . In addition, the spectral changes identified by 31 P MRS in tumour tissue have been seen at a lesser magnitude in the tumour host tissues [24] . Hepatic 31 P MRS has been translated to the in vivo setting by Cox and colleagues and others [25] .…”

mentioning

confidence: 99%

“…The observed hepatic in vivo MR spectrum demonstrated a significantly raised PME/PDE ratio in tumour compared to controls. These peaks are thought to comprise increased PE and PC, and decreased GPE and GPC respectively, as elicited by in vitro analysis of tissue extract [24] .…”

mentioning

confidence: 99%

“…P and 1 H MR spectra from aqueous soluble metabolites in extract of liver tumours of different types (hepatocellular carcinoma, colorectal metastases and secondary lung carcinoma) have been compared with samples from normal liver tissue and from histologically normal liver of the tumour host [24] . A significant increase in phosphoethanolamine (PER), phosphocholine (PC), taurine and lactate has been reported, with a reduction in GPE glycerophosphorylethanolamine (GPE) and glycerophosphorylcholine (GPC) in tumours, compared to normal liver controls [24] .…”

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confidence: 99%

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Current and future applications ofin vitromagnetic resonance spectroscopy in hepatobiliary disease

Cox

Sharif²,

Cobbold³

et al. 2006

WJG

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Nuclear magnetic resonance spectroscopy allows the study of cellular biochemistry and metabolism, both in the whole body in vivo and at higher magnetic field strengths in vitro . Since the technique is non-invasive and non-selective, magnetic resonance spectroscopy methodologies have been widely applied in biochemistry and medicine. In vitro magnetic resonance spectroscopy studies of cells, body fluids and tissues have been used in medical biochemistry to investigate pathophysiological processes and more recently, the technique has been used by physicians to determine disease abnormalities in vivo . This highlighted topic illustrates the potential of in vitro magnetic resonance spectroscopy in studying the hepatobiliary system. The role of in vitro proton and phosphorus magnetic resonance spectroscopy in the study of malignant and non-malignant liver disease and bile composition studies are discussed, particularly with reference to correlative in vivo whole-body magnetic resonance spectroscopy applications. In summary, magnetic resonance spectroscopy techniques can provide non-invasive biochemical information on disease severity and pointers to underlying pathophysiological processes. Magnetic resonance spectroscopy holds potential promise as a screening tool for disease biomarkers, as well as assessing therapeutic response.

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A 31P and 1H-NMR investigation in vitro of normal and abnormal human liver

Cited by 84 publications

References 31 publications

Assessing gene expression in vivo: magnetic resonance imaging and spectroscopy

Assessing gene expression in vivo: magnetic resonance imaging and spectroscopy

Liver transplantation: Current and potential applications of magnetic resonance spectroscopy

Current and future applications ofin vitromagnetic resonance spectroscopy in hepatobiliary disease

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