A 38-year-old woman with zosteriform skin lesions

Camprubí, Daniel; Requena‐Méndez, Ana; Rodríguez, Natàlia; Valls, María Eugenia; García‐Herrera, Adriana; Estrach, Teresa; Fustà, Xavier; Diego, Juan García-Bernalt; Fernández, Sara; Muñóz, José

doi:10.1371/journal.pntd.0005906

Cited by 8 publications

(14 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of eggs in the very early phase of schistosomiasis is reported in some papers: as early as 3 weeks after exposure in the lungs [9], 4 weeks in stool [11,45], 5 weeks in a biopsy of the skin [51]. These findings confirm that the oviposition sometimes starts very early, before what was previously thought (6-7 weeks).…”

Section: As and Lung Nodules: Literature Reporting Very Early Oviposition Does Existsupporting

confidence: 65%

“…Our view is that the asymptomatic focal lesions reported in the first phase of primary schistosomal infection (incidentally observed during AS, but likely not responsible for Katayama symptoms) are due to local deposition of clusters of eggs laid by adult female worms in situ, and, therefore, that all nodules/focal lesions, at any time postinfection and at any site, develop as the consequence of this mechanism. The presence of eggs in such nodules was actually demonstrated by different authors [44], starting as early as 3 weeks after infection [9,45,51]. The presence of an adult worm in situ is more difficult to demonstrate.…”

Section: How To Detect the Presence Of Adult Worms In The Sites Of 'Ectopic' Egg Clustersmentioning

confidence: 93%

See 1 more Smart Citation

New Insights on Acute and Chronic Schistosomiasis: Do We Need a Redefinition?

Gobbi

Tamarozzi

Buonfrate

et al. 2020

Trends in Parasitology

View full text Add to dashboard Cite

Section: As and Lung Nodules: Literature Reporting Very Early Oviposition Does Existsupporting

confidence: 65%

Section: How To Detect the Presence Of Adult Worms In The Sites Of 'Ectopic' Egg Clustersmentioning

confidence: 93%

New Insights on Acute and Chronic Schistosomiasis: Do We Need a Redefinition?

Gobbi

Tamarozzi

Buonfrate

et al. 2020

Trends in Parasitology

View full text Add to dashboard Cite

“…5a). It is known that real-time SmMIT-LAMP works well with clinical samples, since we recently used it on patient’s skin biopsies to confirm the diagnosis of ectopic cutaneous schistosomiasis 48 . Both fresh and desiccated SmMIT-LAMP mixtures yielded amplification signals for S .…”

Section: Resultsmentioning

confidence: 99%

Progress in loop-mediated isothermal amplification assay for detection of Schistosoma mansoni DNA: towards a ready-to-use test

Diego

Fernández‐Soto

Crego-Vicente

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Schistosomiasis is one of the most prevalent Neglected Tropical Disease, affecting approximately 250 million people worldwide. Schistosoma mansoni is the most important species causing human intestinal schistosomiasis. Despite significant efforts in recent decades, the global disease burden of schistosomiasis remains extremely high. This could partly be attributed to the absence of accurate diagnostic tools, primarily in endemic areas. Loop-mediated isothermal amplification (LAMP) is increasingly used in molecular diagnostics as a field-friendly alternative to many other complex molecular methods and it has been proposed as an ideal candidate for revolutionizing point-of-care molecular diagnostics. In a previous work, a LAMP-based method to detect S. mansoni DNA (SmMIT-LAMP) was developed by our research group for early diagnosis of active schistosomiasis in an experimental infection murine model. The SmMIT-LAMP has been further successfully evaluated in both human stool and snail samples and, recently, in human urine samples. In this study, we developed an important improvement for SmMIT-LAMP molecular assay, transforming it into a cold maintenance dry format suitable for potentially manufacturing as kit for ready-to-use for schistosomiasis diagnosis. This procedure could be applied to create dry LAMP kits for a laboratory setting and for diagnostic applications for other neglected tropical diseases.

show abstract

“…Consequently, the combination of the high efficiency of LAMP with urine samples could be suitable to use not only for well-equipped laboratories, but also for poor-resource laboratories in S. mansoni -endemic areas. Additionally, SmMIT-LAMP was also successfully used on a skin biopsy as a real-time LAMP assay to confirm ectopic cutaneous schistosomiasis caused by S. mansoni , a proof of concept of the usefulness of LAMP in acute schistosomiasis diagnosis [ 54 ].…”

Section: Lamp and Schistosomiasismentioning

confidence: 99%

Loop-Mediated Isothermal Amplification in Schistosomiasis

Diego

Fernández‐Soto

Febrer‐Sendra

et al. 2021

JCM

Self Cite

View full text Add to dashboard Cite

Human schistosomiasis is one of the most important parasitic diseases, causing around 250 million cases (mostly in Africa) and 280,000–500,000 deaths every year. Due to the limited resources and the far-removed nature of many endemic areas, the implementation of new, sensitive and specific diagnostic tools has had little success. This is particularly true for PCR-based molecular methods that require expensive equipment and trained personnel to be executed. Loop-mediated isothermal amplification (LAMP) along with other isothermal techniques appeared in the early 21st century as an alternative to those methods, overcoming some of the aforementioned limitations and achieving a more inexpensive diagnostic. However, to this date, neither LAMP nor any other isothermal technique have signified a meaningful change in the way schistosomiasis diagnosis is routinely performed. Here, we present the recent developments in LAMP-based schistosomiasis diagnosis. We expose the main advantages and disadvantages of LAMP technology over PCR and other classical diagnostic methods focusing in various research approaches on intermediate hosts, animal models and patients. We also examine its potential clinical application in post-therapy monitoring, as well as its usefulness as a point-of-care test.

show abstract

A 38-year-old woman with zosteriform skin lesions

Cited by 8 publications

References 8 publications

New Insights on Acute and Chronic Schistosomiasis: Do We Need a Redefinition?

New Insights on Acute and Chronic Schistosomiasis: Do We Need a Redefinition?

Progress in loop-mediated isothermal amplification assay for detection of Schistosoma mansoni DNA: towards a ready-to-use test

Loop-Mediated Isothermal Amplification in Schistosomiasis

Contact Info

Product

Resources

About