A 3D approach to reproduction

Brevini, Tiziana A. L.; Pennarossa, G.; Gandolfi, F.

doi:10.1016/j.theriogenology.2020.01.020

Cited by 13 publications

(8 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular, the cell type selected for our re-seeding experiments well fits with the possible use of iPS-derived ovarian cells (obtained from recipient dermal fibroblasts) to repopulated decellularized ovarian tissue from a xenogeneic source or from human cadaveric organ donors [39]. Although, these results are still preliminary, they pave the way toward further research that will use primary ovarian cell populations for bioscaffold re-seeding, with interesting implications in the field of reproductive biology and biotechnology [55]. In particular, this novel decellularization protocol, that combines physical and chemical methods and preserves shape, architecture, and ECM of the original organ, may represent the first step toward the obtainment of whole-ovary bioscaffolds that may constitute a suitable niche for ex vivo culture of ovarian cells and follicles, as well as a promising tool for the reconstruction of a bioengineered ovary.…”

Section: Discussionmentioning

confidence: 65%

Whole-ovary decellularization generates an effective 3D bioscaffold for ovarian bioengineering

Pennarossa

Ghiringhelli

Gandolfi

et al. 2020

J Assist Reprod Genet

Self Cite

View full text Add to dashboard Cite

Purpose To develop a new protocol for whole-ovary decellularization for the production of a 3D bioscaffold suitable for in vitro/ ex vivo studies and for the reconstruction of a bioengineered ovary. Methods Porcine ovaries were subjected to the decellularization process (DECELL; n = 20) that involved a freeze-thaw cycle, followed by sequential incubations in 0.5% SDS for 3 h, 1% Triton X-100 for 9 h, and 2% deoxycholate for 12 h. Untreated ovaries were used as a control (CTR; n = 6). Both groups were analyzed to evaluate cell and DNA removal as well as ECM preservation. DECELL bioscaffolds were assessed for cytotoxicity and cell homing ability. Results DECELL ovaries maintained shape and homogeneity without any deformation, while their color turned from red to white. Histological staining and DNA quantification confirmed a decrease of 98.11% in DNA content, compared with the native tissue (CTR). Histochemical assessments demonstrated the preservation of intact ECM microarchitecture after the decellularization process. This was also confirmed by quantitative analysis of collagen, elastin, and GAG contents. DECELL bioscaffold showed no cytotoxic effects in co-culture and, when re-seeded with homologous fibroblasts, encouraged a rapid cell adhesion and migration, with repopulating cells increasing in number and aggregating in cluster-like structures, consistent with its ability to sustain cell adherence, proliferation, and differentiation. Conclusion The protocol described allows for the generation of a 3D bioscaffold that may constitute a suitable model for ex vivo culture of ovarian cells and follicles, as well as a promising tool for the reconstruction of a bioengineered ovary.

show abstract

Section: Discussionmentioning

confidence: 65%

Whole-ovary decellularization generates an effective 3D bioscaffold for ovarian bioengineering

Pennarossa

Ghiringhelli

Gandolfi

et al. 2020

J Assist Reprod Genet

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recent studies also suggest that the non-cellular compartment of the tissues, the extracellular matrix (ECM), may play a key role in aging progression [ 7 ]. Indeed, during the last years, it has been clearly demonstrated that ECM contributes not only to physical scaffolding and structural support but also provides biochemical and biomechanical stimuli directly influencing cell behaviour [ 8 ]. Cells are indeed able to respond to physical and mechanical cues exerted by the surrounding environment modifying their own morphology, polarity, adhesion, migration, growth, gene expression, and functions [ 9 , 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds

et al. 2022

Self Cite

View full text Add to dashboard Cite

Advances in medical care, improvements in sanitation, and rising living standards contribute to increased life expectancy. Although this reflects positive human development, it also poses new challenges. Among these, reproductive aging is gradually becoming a key health issue because the age of menopause has remained constant at ~50 years, leading women to live longer in suboptimal endocrine conditions. An adequate understanding of ovarian senescence mechanisms is essential to prevent age-related diseases and to promote wellbeing, health, and longevity in women. We here analyze the impact of aging on the ovarian extracellular matrix (ECM), and we demonstrate significant changes in its composition and organization with collagen, glycosaminoglycans, and laminins significantly incremented, and elastin, as well as fibronectin, decreased. This is accompanied by a dynamic response in gene expression levels of the main ECM- and protease-related genes, indicating a direct impact of aging on the transcription machinery. Furthermore, in order to study the mechanisms driving aging and identify possible strategies to counteract ovarian tissue degeneration, we here described the successful production of a 3D ECM-based biological scaffold that preserves the structural modifications taking place in vivo and that represents a powerful high predictive in vitro model for reproductive aging and its prevention.

show abstract

“…The architectural organisation of collagen, like orthogonally arranged collagen sheets and collagen fibril bundles is often very simplified and not a perfect copy for a gradient of various factors (He et al, 2016). One of the major problems in tissue engineering is the lack of repeatability and standardisation of 3D models to ensure the predictability of results (Brevini et al, 2020). The problem of using 3D systems on a high-throughput scale is currently the greatest challenge facing scientists designing 3D systems.…”

Section: Discussionmentioning

confidence: 99%

3D Cell Culture Technology – A New Insight Into in Vitro Research – A Review

Sośniak¹,

Opiela

2021

Annals of Animal Science

View full text Add to dashboard Cite

Most in vitro cell-based research is based on two-dimensional (2D) systems where growth and development take place on a flat surface, which does not reflect the natural environment of the cells. The imperfection and limitations of culture in 2D systems eventually led to the creation of three-dimensional (3D) culture models that more closely reproduce the actual conditions of physiological cell growth. Since the inception of 3D culture technology, many culture models have been developed, such as technologies of multicellular spheroids, organoids, and organs on chips in the technology of scaffolding, hydrogels, bio-printing and liquid media. In this review we will focus on the advantages and disadvantages of the 2D vs. 3D cell cultures technologies. We will also try to sum up available 3D cultures systems and materials for building 3D scaffolds.

show abstract

A 3D approach to reproduction

Cited by 13 publications

References 73 publications

Whole-ovary decellularization generates an effective 3D bioscaffold for ovarian bioengineering

Whole-ovary decellularization generates an effective 3D bioscaffold for ovarian bioengineering

Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds

3D Cell Culture Technology – A New Insight Into in Vitro Research – A Review

Contact Info

Product

Resources

About