A 3D‐Bioprinted Vascularized Glioblastoma‐on‐a‐Chip for Studying the Impact of Simulated Microgravity as a Novel Pre‐Clinical Approach in Brain Tumor Therapy

Silvani, Giulia; Basirun, Carin; Wu, Huawei; Mehner, Christine; Poole, Kate; Bradbury, Peta; Chou, Joshua

doi:10.1002/adtp.202100106

Cited by 34 publications

(34 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This in turn confirm that GBM spheroid formation, typical of GSC growth is gravity dependent, so that microgravity compromises GBM basic cell functions and mechanisms (Silvani et al, 2021). An innovative series of research investigations is making further advancements in cancer therapy using the peculiar microgravity space environment aboard the ISS.…”

Section: Effects Of Microgravity On Cns Tumorsmentioning

confidence: 56%

“…Using the RPM, it would be possible to observe that the absence of gravity inhibits GBM cell invasion and aggregation, without affecting cell viability. This in turn confirm that GBM spheroid formation, typical of GSC growth is gravity dependent, so that microgravity compromises GBM basic cell functions and mechanisms (Silvani et al, 2021).…”

Section: Microgravity Impact On Cnsmentioning

confidence: 60%

See 1 more Smart Citation

Space flight and central nervous system: Friends or enemies? Challenges and opportunities for neuroscience and neuro‐oncology

Marfia

Navone

Guarnaccia

et al. 2022

J of Neuroscience Research

View full text Add to dashboard Cite

Space environment provides many challenges to pilots, astronauts, and space scientists, which are constantly subjected to unique conditions, including microgravity, radiations, hypoxic condition, absence of the day and night cycle, etc. These stressful stimuli have the potential to affect many human physiological systems, triggering physical and biological adaptive changes to re-establish the homeostatic state. A particular concern regards the risks for the effects of spaceflight on the central nervous system (CNS), as several lines of evidence reported a great impact on neuroplasticity, cognitive functions, neurovestibular system, short-term memory, cephalic fluid shift, reduction in motor function, and psychological disturbances, especially during longterm missions. Aside these potential detrimental effects, the other side of the coin reflects the potential benefit of applicating space-related conditions on Earth-based life sciences, as cancer research. Here, we focused on examining the effect of real and simulated microgravity on CNS functions, both in humans and in cellular models, browsing the different techniques to experience or mime microgravity on-ground.Increasing evidence demonstrate that cancer cells, and brain cancer cells in particular, are negatively affected by microgravity, in terms of alteration in cell morphology, proliferation, invasion, migration, and apoptosis, representing an advancing novel side of space-based investigations. Overall, deeper understandings about the mechanisms by which space environment influences CNS and tumor biology may be promisingly translated into many clinical fields, ranging from aerospace medicine to neuroscience and oncology, representing an enormous pool of knowledge for the implementation of countermeasures and therapeutic applications.

show abstract

Section: Effects Of Microgravity On Cns Tumorsmentioning

confidence: 56%

Section: Microgravity Impact On Cnsmentioning

confidence: 60%

Space flight and central nervous system: Friends or enemies? Challenges and opportunities for neuroscience and neuro‐oncology

Marfia

Navone

Guarnaccia

et al. 2022

J of Neuroscience Research

View full text Add to dashboard Cite

show abstract

“…As such, 3D culturing cells using Transwell systems has been suggested as a potential solution, [186] thereby enabling the assessment of barrier functions via TEER and permeability assays. As recently demonstrated by Silvani et al (Figure 4f), [177] a more advanced approach will be to combine bioprinting techniques with microfluidic OoC platforms to permit the culturing of BMECs with shear stress and the parenchymal cells in 3D environments with reduced chip-to-chip variations.…”

Section: D Versus 3d Culturementioning

confidence: 99%

mentioning

confidence: 99%

Organ‐On‐A‐Chip Models of the Blood–Brain Barrier: Recent Advances and Future Prospects

Kawakita

Mandal

Mou

et al. 2022

Small

View full text Add to dashboard Cite

refers to a multicellular unit in the brain including cells of the cerebral vasculature and brain parenchyma. [2] As part of the NVU, the blood-brain barrier (BBB) acts as a physiological barrier at the interface between peripheral blood circulation and the central nervous system (CNS). The BBB is indispensable for the proper CNS function and regulation of CNS homeostasis; it controls the transport of substances between the CNS and the blood via various transport mechanisms. [3] The BBB also protects the brain from neurotoxic plasma components, certain chemicals, and pathogens. Functional and structural changes of the BBB are implicated in several neurological diseases and disorders, which have severely impacted individuals worldwide. For example, there are currently estimated to be about 5.7 million people living with Alzheimer's disease (AD), and the number is projected to reach 13.8 million by 2050. [4] Stroke is the second leading cause of death and the third most common cause of disability. [5] Glioblastoma multiforme (GBM), which accounts for almost 50% of all brain tumors (i.e., glioma), is relatively rare with a global incidence rate of 3.19 per 100 000 people; however, it has a devastatingly poor prognosis with a typical survival rate of 14-15 months rendering it a critical public health problem. [6] The human brain and central nervous system (CNS) present unique challenges in drug development for neurological diseases. One major obstacle is the blood-brain barrier (BBB), which hampers the effective delivery of therapeutic molecules into the brain while protecting it from blood-born neurotoxic substances and maintaining CNS homeostasis. For BBB research, traditional in vitro models rely upon Petri dishes or Transwell systems. However, these static models lack essential microenvironmental factors such as shear stress and proper cell-cell interactions. To this end, organ-on-a-chip (OoC) technology has emerged as a new in vitro modeling approach to better recapitulate the highly dynamic in vivo human brain microenvironment socalled the neural vascular unit (NVU). Such BBB-on-a-chip models have made substantial progress over the last decade, and concurrently there has been increasing interest in modeling various neurological diseases such as Alzheimer's disease and Parkinson's disease using OoC technology. In addition, with recent advances in other scientific technologies, several new opportunities to improve the BBB-on-a-chip platform via multidisciplinary approaches are available. In this review, an overview of the NVU and OoC technology is provided, recent progress and applications of BBB-on-a-chip for personalized medicine and drug discovery are discussed, and current challenges and future directions are delineated.The ORCID identification number(s) for the author(s) of this article can be found under

show abstract

“…The suggested etiology was hyperperfusion, together with oxidative stress (Bailey et al, 2020). Silvani et al (2021) developed an innovative hybrid in vitro vascularized glioblastoma-on-a-chip model. Under simulated microgravity conditions, the system demonstrated a significant cell morphological and mechanotransduction response, representing a tool for investigating cancer mechanobiology and the blood-tumor barrier.…”

Section: Microgravity and The Blood-brain Barriermentioning

confidence: 99%

The Blood-Brain Barrier in Space: Implications for Space Travelers and for Human Health on Earth

Amselem

Eyal

2022

Front. Drug. Deliv.

View full text Add to dashboard Cite

Astronauts have flown to space for decades, but the effects of spaceflight on human health have not been fully clarified yet. Several pathologies have only been detected after it has become customary for astronauts to spend months rather than days in space and with the advance of inflight monitoring. Examples include the neuro-ocular spaceflight associated syndrome, changes to the brain’s white matter, and, more recently, altered cerebral blood flow and related hypercoagulability. This review outlines spaceflight-induced brain disorders in astronauts and putative contributing factors. It next presents ongoing and upcoming studies of the BBB onboard space platforms. Finally, it describes how the space environment can be harnessed for improving drug-delivery across the BBB for humans both in space and on Earth.

show abstract

A 3D‐Bioprinted Vascularized Glioblastoma‐on‐a‐Chip for Studying the Impact of Simulated Microgravity as a Novel Pre‐Clinical Approach in Brain Tumor Therapy

Cited by 34 publications

References 87 publications

Space flight and central nervous system: Friends or enemies? Challenges and opportunities for neuroscience and neuro‐oncology

Space flight and central nervous system: Friends or enemies? Challenges and opportunities for neuroscience and neuro‐oncology

Organ‐On‐A‐Chip Models of the Blood–Brain Barrier: Recent Advances and Future Prospects

The Blood-Brain Barrier in Space: Implications for Space Travelers and for Human Health on Earth

Contact Info

Product

Resources

About