A 3D hanging spheroid-filter plate for high-throughput drug testing and CAR T cell cytotoxicity assay

Chen, Zhenzhong; Han, Seokgyu; Kim, Sein; Lee, Chanyang; Sanny, Arleen; Tan, Andy Hee-Meng; Park, Sungsu

doi:10.1039/d3an01904g

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2024

DOI: 10.1039/d3an01904g

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A 3D hanging spheroid-filter plate for high-throughput drug testing and CAR T cell cytotoxicity assay

Zhenzhong Chen,

Seokgyu Han,

Sein Kim

et al.

Abstract: 3D Hanging spheroid-filter plate (3DHS-FP) comprises a 3D hanging dripper (top) and a spheroid filter plate (bottom). It is designed to streamline spheroid formation and the effortless separation of unbound and dead cells from tumor spheroids.

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Cited by 3 publications

References 31 publications

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Channel-assembling tumor microenvironment on-chip for evaluating anticancer drug efficacy

Lee,

Kim,

Jung

et al. 2025

Journal of Controlled Release

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Channel-assembling tumor microenvironment on-chip for evaluating anticancer drug efficacy

Lee,

Kim,

Jung

et al. 2025

Journal of Controlled Release

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Reshaping the tumor immune microenvironment to improve CAR-T cell-based cancer immunotherapy

Xia,

Yang,

et al. 2024

Mol Cancer

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Recapitulating Glioma Stem Cell Niches Using 3D Spheroid Models for Glioblastoma Research

Jo,

Lee,

Kim

et al. 2024

Biosensors

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Glioblastoma multiforme (GBM) is among the most aggressive brain cancers, and it contains glioma stem cells (GSCs) that drive tumor initiation, progression, and recurrence. These cells resist conventional therapies, contributing to high recurrence rates in GBM patients. Developing in vitro models that mimic the tumor microenvironment (TME), particularly the GSC niche, is crucial for understanding GBM growth and therapeutic resistance. Three-dimensional (3D) spheroid models provide a more physiologically relevant approach than traditional two-dimensional (2D) cultures, recapitulating key tumor features like hypoxia, cell heterogeneity, and drug resistance. This review examines scaffold-free and scaffold-based methods for generating 3D GBM spheroids, focusing on their applications in studying the cancer stem cell niche. The discussion encompasses methods such as the hanging drop, low-adhesion plates, and magnetic levitation, alongside advancements in embedding spheroids within extracellular matrix-based hydrogels and employing 3D bioprinting to fabricate more intricate tumor models. These 3D culture systems offer substantial potential for enhancing our understanding of GBM biology and devising more effective targeted therapies.

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A 3D hanging spheroid-filter plate for high-throughput drug testing and CAR T cell cytotoxicity assay

Abstract: 3D Hanging spheroid-filter plate (3DHS-FP) comprises a 3D hanging dripper (top) and a spheroid filter plate (bottom). It is designed to streamline spheroid formation and the effortless separation of unbound and dead cells from tumor spheroids.

Cited by 3 publications

References 31 publications

Channel-assembling tumor microenvironment on-chip for evaluating anticancer drug efficacy

Channel-assembling tumor microenvironment on-chip for evaluating anticancer drug efficacy

Reshaping the tumor immune microenvironment to improve CAR-T cell-based cancer immunotherapy

Recapitulating Glioma Stem Cell Niches Using 3D Spheroid Models for Glioblastoma Research

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