A 3D <i>in vitro</i> pericyte-supported microvessel model: visualisation and quantitative characterisation of multistep angiogenesis

Lee, Eujin; Takahashi, Haruko; Pauty, Joris; Kobayashi, Masayoshi; Kato, Keisuke; Kabara, Maki; Kawabe, Jun‐ichi; Matsunaga, Yukiko T.

doi:10.1039/c7tb03239k

Cited by 28 publications

(26 citation statements)

References 33 publications

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“…1D). We note that, unlike other previously described engineered blood vessel models with mural cells (22)(23)(24)(25), the major advantage of this newly developed experimental system is a highly controlled juxtaposition between ECs and PCs from the outset of the experiment, without any additional treatment used to recruit PCs onto endothelium from the surrounding gel. The cells were accessible to high-resolution imaging modalities, including confocal microscopy, which was used throughout the experimental analysis presented here.…”

Section: Resultsmentioning

confidence: 99%

Pericytes enable effective angiogenesis in the presence of proinflammatory signals

Kang

Bocci

Jolly

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Angiogenesis frequently occurs in the context of acute or persistent inflammation. The complex interplay of proinflammatory and proangiogenic cues is only partially understood. Using an experimental model, permitting exposure of developing blood vessel sprouts to multiple combinations of diverse biochemical stimuli and juxtacrine cell interactions, we present evidence that a proinflammatory cytokine, tumor necrosis factor (TNF), can have both proangiogenic and antiangiogenic effects, depending on the dose and the presence of pericytes. In particular, we find that pericytes can rescue and enhance angiogenesis in the presence of otherwise-inhibitory high TNF doses. This sharp switch from proangiogenic to antiangiogenic effect of TNF observed with an escalating dose of this cytokine, as well as the effect of pericytes, are explained by a mathematical model trained on the biochemical data. Furthermore, this model was predictive of the effects of diverse combinations of proinflammatory and antiinflammatory cues, and variable pericyte coverage. The mechanism supports the effect of TNF and pericytes as modulating signaling networks impinging on Notch signaling and specification of the Tip and Stalk phenotypes. This integrative analysis elucidates the plasticity of the angiogenic morphogenesis in the presence of diverse and potentially conflicting cues, with immediate implications for many physiological and pathological settings.

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Section: Resultsmentioning

confidence: 99%

Pericytes enable effective angiogenesis in the presence of proinflammatory signals

Kang

Bocci

Jolly

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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“…1D). We note that, unlike other previously described blood vessel models with mural cells 23-26 , the major advantage of this newly developed experimental system is to enable a highly controlled juxtaposition and possible interaction between endothelial cells and pericytes from the outset of the experiment, without any additional treatment used to recruit pericytes onto endothelium from the surrounding gel. The cells were accessible to high resolution imaging modalities, including confocal microscopy, which was used throughout the experimental analysis presented here.…”

Section: Resultsmentioning

confidence: 99%

Pericytes enable effective angiogenesis in the presence of pro-inflammatory signals

Kang

Bocci

Jolly

et al. 2019

Preprint

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19Angiogenesis frequently occurs in the context of acute or persistent inflammation. The complex 20 interplay of pro-inflammatory and pro-angiogenic cues is only partially understood. Using a new 21 experimental model permitting exposure of developing blood vessel sprouts to multiple 22 combinations of diverse biochemical stimuli and juxtacrine cell interactions, we present evidence 23 that a pro-inflammatory cytokine, tumor necrosis factor (TNF), can have both pro-and anti-24 angiogenic effects, depending on the dose and the presence of pericytes. In particular, we find that 25 pericytes can rescue and enhance angiogenesis in the presence of otherwise inhibitory high TNF 26doses. This sharp switch from pro-to anti-angiogenic effect of TNF observed with an escalating 27 dose of this cytokine, as well as the effect of pericytes are explained by a mathematical model 28 trained on the biochemical data. Furthermore, this model was predictive of the effects of diverse 29 combinations of pro-and anti-inflammatory cues, and variable pericyte coverage. The mechanism 30 supports the effect of TNF and pericytes as modulating signaling networks impinging in Notch 31 signaling and specification of the Tip and Stalk phenotypes. This integrative analysis elucidates 32 the plasticity of the angiogenic morphogenesis in the presence of diverse and potentially 33 conflicting cues, with immediate implications for many physiological and pathological settings. 34 35

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“…Optical coherence tomography (OCT) is a non-invasive cross-sectional imaging process used in biological systems [13][14][15]. At present, it is used in ophthalmology, especially for funduscopic examination of the retina.…”

Section: Textmentioning

confidence: 99%

Three-dimensional live imaging of bovine embryos by optical coherence tomography

Masuda¹,

Hasebe

Kuromi

et al. 2021

J. Reprod. Dev.

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While embryo transfer (ET) is widely practiced, many of the transferred embryos fail to develop in cattle. To establish a more effective method for selecting bovine embryos for ET, here we quantified morphological parameters of living embryos using three-dimensional (3D) images non-invasively captured by optical coherence tomography (OCT). Seven Japanese Black embryos produced by in vitro fertilization that had reached the expanded blastocyst stage after 7 days of culture were transferred after imaged by OCT. Twenty-two parameters, including thickness and volumes of the inner cell mass, trophectoderm, and zona pellucida, and volumes

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A 3D in vitro pericyte-supported microvessel model: visualisation and quantitative characterisation of multistep angiogenesis

Abstract: A 3D in vitro microvessel model consisting of pericytes and endothelial cells which enables visualization of the multistep process of angiogenesis induced by VEGF.

Cited by 28 publications

References 33 publications

Pericytes enable effective angiogenesis in the presence of proinflammatory signals

Pericytes enable effective angiogenesis in the presence of proinflammatory signals

Pericytes enable effective angiogenesis in the presence of pro-inflammatory signals

Three-dimensional live imaging of bovine embryos by optical coherence tomography

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