A 3D-induced pluripotent stem cell-derived human neural culture model to study certain molecular and biochemical aspects of Alzheimer’s disease

Prasannan, Preeti; Siney, Elodie J.; Chatterjee, Shreyasi; Johnston, D.; Shah, Mitul; Mudher, Amritpal; Willaime‐Morawek, Sandrine

doi:10.1007/s44164-022-00038-5

Cited by 3 publications

(2 citation statements)

References 64 publications

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“… 16 Like hESCs, human induced pluripotent stem cells (hiPSCs) can be transformed into almost any cell in the nervous system. hiPSCs have been employed in 3D models of brain injury 40 and neurodegenerative diseases including Parkinson’s disease, 41 Alzheimer’s disease, 42 and Amyotrophic lateral sclerosis. 43 Additionally, the use of hiPSCs is promising in terms of taking a step into the field of personal medicine.…”

Section: Key Parametersmentioning

confidence: 99%

Applications of 3D Bioprinting Technology to Brain Cells and Brain Tumor Models: Special Emphasis to Glioblastoma

Ozbek,

Saybasili,

Ulgen

2024

ACS Biomater. Sci. Eng.

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Primary brain tumor is one of the most fatal diseases. The most malignant type among them, glioblastoma (GBM), has low survival rates. Standard treatments reduce the life quality of patients due to serious side effects. Tumor aggressiveness and the unique structure of the brain render the removal of tumors and the development of new therapies challenging. To elucidate the characteristics of brain tumors and examine their response to drugs, realistic systems that mimic the tumor environment and cellular crosstalk are desperately needed. In the past decade, 3D GBM models have been presented as excellent platforms as they allowed the investigation of the phenotypes of GBM and testing innovative therapeutic strategies. In that scope, 3D bioprinting technology offers utilities such as fabricating realistic 3D bioprinted structures in a layer-by-layer manner and precisely controlled deposition of materials and cells, and they can be integrated with other technologies like the microfluidics approach. This Review covers studies that investigated 3D bioprinted brain tumor models, especially GBM using 3D bioprinting techniques and essential parameters that affect the result and quality of the study like frequently used cells, the type and physical characteristics of hydrogel, bioprinting conditions, cross-linking methods, and characterization techniques.

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Section: Key Parametersmentioning

confidence: 99%

Applications of 3D Bioprinting Technology to Brain Cells and Brain Tumor Models: Special Emphasis to Glioblastoma

Ozbek,

Saybasili,

Ulgen

2024

ACS Biomater. Sci. Eng.

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“…The bioprinting process is controlled and can produce constructs previously determined in size, thickness and shape [199,200,202]. Accordingly, 3D culturing and bioprinting increase the dimension of exploratory evaluations of cell-cell [189,190,203,204], cell-hydrogel [205] and cell-ECM interactions [206] in processes of cell migration [207], shape remodeling [122], differentiation [208,209], neuropathological paradigms [210,211] and so on, with greater chance of compatibility with in vivo conditions.…”

Section: D Culture and Bioprintingmentioning

confidence: 99%

The Relevance of Astrocytic Cell Culture Models for Neuroinflammation in Neurodegeneration Research

Preato,

Pinheiro,

Rosenstock

et al. 2024

Neuroglia

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Astrocytes are the predominant glial cells that provide essential support to neurons and promote microenvironment changes in neuropathological states. Astrocyte and astrocytic-like cell culture have substantially contributed to elucidating the molecular pathways involved in key glial roles, including those relevant to neurodevelopment, brain physiology and metabolism, which are not readily accessible with traditional approaches. The in vitro methodology has also been applied to neuroinflammatory and neurodegeneration contexts, revealing cellular changes involved in brain dysfunction. Astrocytes studies in culture started with primary cell approaches using embryonic and postmortem tissue. Further developments included newborn rodent primary cells, cell lines and immortalized astrocytes, which resulted in homogeneous cell-type preparations grown on flat surfaces. To overcome some in vitro shortcomings, tridimensional bioprinted models and organoid culture enabled the mimicking of tissue cellular arrangements and, above these achievements, complex astrocyte cell culture can be generated from induced pluripotent stem cells (iPSCs) to model diseases. These unprecedented breakthroughs allowed the development of platforms to test new therapies in brain cells derived from human material noninvasively obtained from live patients. In this work, we reviewed the most studied astrocytic cell models for discussing limitations, advantages and reliable experimental readouts for neuroinflammation in neurodegeneration research.

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Highly aligned bacterial cellulose/PPy gradient conductive membranes for directed cell differentiation under electrical stimulation

Wang,

Qi,

Wang

et al. 2024

Preprint

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Both of the topographical and gradient conductive cues can influence the cellular activity and thereby tissue regeneration. However, they have not been combined simultaneously onto biomaterial with electrical stimulation to demonstrate the synergistic role so far. Herein, we assume that a bacterial cellulose (BC) -based membrane by incorporating aligned nanofibers and a concentration gradient of polypyrrole (PPy) with electrical stimulation treatment will promote cell differentiation in peripheral nerve regeneration. The results showed that PPy were successfully deposited on the aligned BC/PPy with gradient conductive structure, which exhibited good mechanical property, thermal stability, the gradient decrease in surface resistance, gradient increase in surface current from the up to down segments, as well as excellent biocompatibility. Especially, the membranes promoted the gradient proliferation and differentiation of PC12 cells in vitro. Importantly, combined with electric field (EF), the aligned BC/PPy gradient conductive membranes synergistically directed the differentiation of PC12 cells. The overall results suggest the aligned BC/PPy gradient conductive membranes with EF could be a promising therapeutic strategy to guide cellular activities for peripheral nerve regeneration.

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A 3D-induced pluripotent stem cell-derived human neural culture model to study certain molecular and biochemical aspects of Alzheimer’s disease

Cited by 3 publications

References 64 publications

Applications of 3D Bioprinting Technology to Brain Cells and Brain Tumor Models: Special Emphasis to Glioblastoma

Applications of 3D Bioprinting Technology to Brain Cells and Brain Tumor Models: Special Emphasis to Glioblastoma

The Relevance of Astrocytic Cell Culture Models for Neuroinflammation in Neurodegeneration Research

Highly aligned bacterial cellulose/PPy gradient conductive membranes for directed cell differentiation under electrical stimulation

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