2022
DOI: 10.1007/s00216-021-03835-1
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A 3D printable adapter for solid-state fluorescence measurements: the case of an immobilized enzymatic bioreceptor for organophosphate pesticides detection

Abstract: The widespread use of pesticides in the last decades and their accumulation into the environment gave rise to major environmental and human health concerns. To address this topic, the scientific community pointed out the need to develop methodologies to detect and measure the presence of pesticides in different matrices. Biosensors have been recently explored as fast, easy, and sensitive methods for direct organophosphate pesticides monitoring. Thus, the present work aimed at designing and testing a 3D printed… Show more

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Cited by 9 publications
(15 citation statements)
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“…p < 0.0001, Figure 2b) and high (Y = 12.57X + 98.1, R 2 = 0.99, F(1,3) = 2437, p < 0.0001, Figure 2c) protein amounts. Our results agree with previous results, where linear behaviour of the labelled EST2-S35C was obtained with different fluorescence-based methods [33].…”
Section: Fluorescence Resonance Energy Transfer (Fret) Measurementssupporting
confidence: 93%
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“…p < 0.0001, Figure 2b) and high (Y = 12.57X + 98.1, R 2 = 0.99, F(1,3) = 2437, p < 0.0001, Figure 2c) protein amounts. Our results agree with previous results, where linear behaviour of the labelled EST2-S35C was obtained with different fluorescence-based methods [33].…”
Section: Fluorescence Resonance Energy Transfer (Fret) Measurementssupporting
confidence: 93%
“…A significant linear increase in the fluorescence intensity of IAEDANS after energy transfer from tryptophan was observed for both low (Y = 29.12X + 8.916, R 2 = 0.99, F (1,3) = 899.9, p < 0.0001, Figure 2b) and high (Y = 12.57X + 98.1, R 2 = 0.99, F (1,3) = 2437, p < 0.0001, Figure 2c) protein amounts. Our results agree with previous results, where linear behaviour of the labelled EST2-S35C was obtained with different fluorescence-based methods [33]. p < 0.0001, Figure 2b) and high (Y = 12.57X + 98.1, R 2 = 0.99, F(1,3) = 2437, p < 0.0001, Figure 2c) protein amounts.…”
Section: Fluorescence Resonance Energy Transfer (Fret) Measurementssupporting
confidence: 93%
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“…To overcome this problem, the enzyme was immobilised on a membrane which was placed onto a support in a 3D adapter designed as part of the extracurricular activities developed during the 1‐year fellowship programme. The 3D support allows the washing of the membrane to remove unwanted substances such as pigments, improving the fluorescence measurements for food samples and avoiding fluorescence interferences in the aqueous solution (Rodrigues et al, 2021 ).…”
Section: Discussionmentioning
confidence: 99%
“…In the first part of the work programme, the fellow carried out the overexpression in mesophilic host Escherichia coli strain BL21 (DE3) of a mutant of the thermostable esterase‐2 (EST2) from Alicyclobacillus acidocaldariu s (Febbraio et al, 2011 ), to which the serine 35 was replaced by a cysteine residue (EST2‐S35C) near the catalytic site. EST2‐S35C was extracted and purified following a protocol already described in Carullo et al ( 2018 ), with slight modifications (Rodrigues et al, 2021 ). The microorganism was grown in an appropriate medium, the biomass was recovered by centrifugation and the protein was extracted by a sonication step.…”
Section: Description Of Work Programmementioning
confidence: 99%