A 3D-printed, multi-modal microfluidic device for measuring nitric oxide and ATP release from flowing red blood cells

Hayter, Elizabeth A.; Azibere, Samuel; Skrajewski, Lauren; Soule, Logan D; Spence, Dana M.; Martin, R. Scott

doi:10.1039/d2ay00931e

Cited by 8 publications

(3 citation statements)

References 75 publications

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“…An active research area using LOCs is measuring RBC metabolite release. Two examples feature measurements of the ability of stored RBCs to i) release oxygen ( 112 ) and ii) release ATP and nitric oxide (NO) in circulation rather than measuring the intracellular levels ( 113 , 114 ). Measurement of oxygen release rate is possible using precisely controlled microenvironments at the single-cell level in LOCs via luminescent probes ( 112 ).…”

Section: Loc Technologies For the Assessment Of Stored Rbcsmentioning

confidence: 99%

“…Measurement of oxygen release rate is possible using precisely controlled microenvironments at the single-cell level in LOCs via luminescent probes ( 112 ). Similarly, ATP and NO can be facilitated by the chemiluminescent detection of the luciferin/luciferase reaction for ATP and amperometric ( 113 ) or fluorogenic detection for NO ( 114 ). Indeed, LOCs can measure ATP and NO release from RBCs ( 113 ) and quantify the downstream effects of ATP release—stimulation of NO release by endothelial cells—by integrated microfluidic components ( 114 ).…”

Section: Loc Technologies For the Assessment Of Stored Rbcsmentioning

confidence: 99%

“…Similarly, ATP and NO can be facilitated by the chemiluminescent detection of the luciferin/luciferase reaction for ATP and amperometric ( 113 ) or fluorogenic detection for NO ( 114 ). Indeed, LOCs can measure ATP and NO release from RBCs ( 113 ) and quantify the downstream effects of ATP release—stimulation of NO release by endothelial cells—by integrated microfluidic components ( 114 ). These studies, together, have assessed the quality of stored RBCs with different glucose levels and compared normoxic and hypoxic conditions for storage.…”

Section: Loc Technologies For the Assessment Of Stored Rbcsmentioning

confidence: 99%

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Assessment of stored red blood cells through lab-on-a-chip technologies for precision transfusion medicine

Işıksaçan

D’Alessandro

Wolf

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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Transfusion of red blood cells (RBCs) is one of the most valuable and widespread treatments in modern medicine. Lifesaving RBC transfusions are facilitated by the cold storage of RBC units in blood banks worldwide. Currently, RBC storage and subsequent transfusion practices are performed using simplistic workflows. More specifically, most blood banks follow the “first-in-first-out” principle to avoid wastage, whereas most healthcare providers prefer the “last-in-first-out” approach simply favoring chronologically younger RBCs. Neither approach addresses recent advances through -omics showing that stored RBC quality is highly variable depending on donor-, time-, and processing-specific factors. Thus, it is time to rethink our workflows in transfusion medicine taking advantage of novel technologies to perform RBC quality assessment. We imagine a future where lab-on-a-chip technologies utilize novel predictive markers of RBC quality identified by -omics and machine learning to usher in a new era of safer and precise transfusion medicine.

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