A 3D-Printed Sensor for Monitoring Biosignals in Small Animals

Cho, Sung‐Joon; Byun, Donghak; Nam, Tai‐Seung; Choi, Seok‐Yong; Lee, Byung-Geun; Kim, Myeong-Kyu; Kim, Sohee

doi:10.1155/2017/9053764

Cited by 18 publications

(6 citation statements)

References 27 publications

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“…Beyond its applications in clinical settings, 3D printing has made significant contributions to advancing preclinical research (Goyanes et al, 2018;Wang et al, 2018). In specific scenarios, 3D printing serves as a viable substitute for traditional microfabrication processes (Cho et al, 2017).…”

Section: Open Accessmentioning

confidence: 99%

3D-printed weight holders design and testing in mouse models of spinal cord injury

De Vincentiis,

Merighi,

Blümler

et al. 2024

Front. Drug Deliv.

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This paper details the comprehensive design and prototyping of a 3D-printed wearable device tailored for mouse models which addresses the need for non-invasive applications in spinal cord studies and therapeutic treatments. Our work was prompted by the increasing demand for wearable devices in preclinical research on freely behaving rodent models of spinal cord injury. We present an innovative solution that employs compliant 3D-printed structures for stable device placement on the backs of both healthy and spinal cord-injured mice. In our trial, the device was represented by two magnets that applied passive magnetic stimulation to the injury site. This device was designed to be combined with the use of magnetic nanoparticles to render neurons or neural cells sensitive to an exogenous magnetic field, resulting in the stimulation of axon growth in response to a pulling force. We show different design iterations, emphasizing the challenges faced and the solutions proposed during the design process. The iterative design process involved multiple phases, from the magnet holder (MH) to the wearable device configurations. The latter included different approaches: a “Fitbit”, “Belt”, “Bib”, and ultimately a “Cape”. Each design iteration was accompanied by a testing protocol involving healthy and injured mice, with qualitative assessments focusing on animal wellbeing. Follow-up lasted for at least 21 consecutive days, thus allowing animal welfare to be accurately monitored. The final Cape design was our best compromise between the need for a thin structure that would not hinder movement and the resistance required to maintain the structure at the correct position while withstanding biting and mechanical stress. The detailed account of the iterative design process and testing procedures provides valuable insights for researchers and practitioners engaged in the development of wearable devices for mice, particularly in the context of spinal cord studies and therapeutic treatments. Finally, in addition to describing the design of a 3D-printed wearable holder, we also outline some general guidelines for the design of wearable devices.

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Section: Open Accessmentioning

confidence: 99%

3D-printed weight holders design and testing in mouse models of spinal cord injury

De Vincentiis,

Merighi,

Blümler

et al. 2024

Front. Drug Deliv.

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“…Moreover, not only single sensors are produced by 3D printing technology, but also whole systems-for example, non-invasive electroencephalography (EEG) sensors for zebrafish monitoring for the investigation of some diseases [106,107]. Thanks to AM, the material cost and fabrication time could be reduced significantly in comparison to the traditional method of manufacturing [106,107]. Another example in this area is the design of an underwater 3D scanner and its manufacturing with this technology [108].…”

Section: Sensorsmentioning

confidence: 99%

Additive Manufacturing in Underwater Applications

Korniejenko,

Gądek,

Dynowski

et al. 2024

Applied Sciences

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Additive manufacturing (AM), commonly named 3D printing, is a promising technology for many applications. It is the most viable option for widespread use in automated construction processes, especially for harsh environments such as underwater. Some contemporary applications of this technology have been tested in underwater environments, but there are still a number of problems to be solved. This study focuses on the current development of 3D printing technology for underwater applications, including the required improvements in the technology itself, as well as new materials. Information about underwater applications involving part fabrication via AM is also provided. The article is based on a literature review that is supplemented by case studies of practical applications. The main findings show that the usage of additive manufacturing in underwater applications can bring a number of advantages—for instance, increasing work safety, limiting the environmental burden, and high efficiency. Currently, only a few prototype applications for this technology have been developed. However, underwater additive manufacturing is a promising tool to develop new, effective applications on a larger scale. The technology itself, as well as the materials used, still require development and optimization.

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“…Examples include higher throughput patch clamp methods ( Dunlop et al, 2008 ; Obergrussberger et al, 2016 ; Obergrussberger et al, 2018 ; Liu et al, 2019 ; Gao et al, 2020 ), in vitro cell culture multi-electrode arrays (MEA) ( Mack et al, 2014 ; Zwartsen et al, 2018 ; Shafer, 2019 ), and use of alternative (non-mammalian) species such as zebrafish ( Milan et al, 2006 ; Meyer et al, 2016 ), or Caenorhabditis elegans ( C. elegans ), ( Richmond and Jorgensen, 1999 ; Goodman et al, 2012 ; Lockery et al, 2012 ). The use of MEAs has been proposed as a method to screen for seizurogenic potential of chemicals/drugs, and has been used in human tissue for epilepsy studies ( Dossi et al, 2014 ; Meyer et al, 2016 ; Cho et al, 2017 ; Bradley and Strock, 2019 ; Fan et al, 2019 ). Additionally, MEAs have been proposed to have some utility in classifying possible mechanisms of actions of chemicals on the neuronal activity ( Mack et al, 2014 ).…”

Section: Futurementioning

confidence: 99%

The Future of Neurotoxicology: A Neuroelectrophysiological Viewpoint

Herr

2021

Front. Toxicol.

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Neuroelectrophysiology is an old science, dating to the 18th century when electrical activity in nerves was discovered. Such discoveries have led to a variety of neurophysiological techniques, ranging from basic neuroscience to clinical applications. These clinical applications allow assessment of complex neurological functions such as (but not limited to) sensory perception (vision, hearing, somatosensory function), and muscle function. The ability to use similar techniques in both humans and animal models increases the ability to perform mechanistic research to investigate neurological problems. Good animal to human homology of many neurophysiological systems facilitates interpretation of data to provide cause-effect linkages to epidemiological findings. Mechanistic cellular research to screen for toxicity often includes gaps between cellular and whole animal/person neurophysiological changes, preventing understanding of the complete function of the nervous system. Building Adverse Outcome Pathways (AOPs) will allow us to begin to identify brain regions, timelines, neurotransmitters, etc. that may be Key Events (KE) in the Adverse Outcomes (AO). This requires an integrated strategy, from in vitro to in vivo (and hypothesis generation, testing, revision). Scientists need to determine intermediate levels of nervous system organization that are related to an AO and work both upstream and downstream using mechanistic approaches. Possibly more than any other organ, the brain will require networks of pathways/AOPs to allow sufficient predictive accuracy. Advancements in neurobiological techniques should be incorporated into these AOP-base neurotoxicological assessments, including interactions between many regions of the brain simultaneously. Coupled with advancements in optogenetic manipulation, complex functions of the nervous system (such as acquisition, attention, sensory perception, etc.) can be examined in real time. The integration of neurophysiological changes with changes in gene/protein expression can begin to provide the mechanistic underpinnings for biological changes. Establishment of linkages between changes in cellular physiology and those at the level of the AO will allow construction of biological pathways (AOPs) and allow development of higher throughput assays to test for changes to critical physiological circuits. To allow mechanistic/predictive toxicology of the nervous system to be protective of human populations, neuroelectrophysiology has a critical role in our future.

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A 3D-Printed Sensor for Monitoring Biosignals in Small Animals

Cited by 18 publications

References 27 publications

3D-printed weight holders design and testing in mouse models of spinal cord injury

3D-printed weight holders design and testing in mouse models of spinal cord injury

Additive Manufacturing in Underwater Applications

The Future of Neurotoxicology: A Neuroelectrophysiological Viewpoint

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