A 4-year survey of dermatomycoses in Tehran from 2006 to 2009

Sadeghi, Golnar; Abouei, M.; Alirezaee, M.; Tolouei, R.; Shams-Ghahfarokhi, Masoomeh; Mostafavi, Ehsan; Razzaghi-Abyaneh, Mehdi

doi:10.1016/j.mycmed.2011.10.001

Cited by 20 publications

(12 citation statements)

References 26 publications

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“…Few studies reported data regarding the predominance of filamentous fungi and yeast over dermatophytes in onychomycosis . According to the considerable body of literature, the most prevalent isolated pathogens detected in nail fungal infections are dermatophytes, followed by yeast and moulds .…”

Section: Discussionmentioning

confidence: 99%

Molecular identification and antifungal susceptibility of clinical fungal isolates from onychomycosis (uncommon and emerging species)

Haghani

Shams-Ghahfarokhi

Dalimi

et al. 2018

Mycoses

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Summary Onychomycosis is a common nail problem, accounting for up to 50% of all nail diseases. The aim of the present study was to determine the species distribution based on the restriction fragment length polymorphism and susceptibility patterns of the causative agents of onychomycosis. This cross‐sectional study was conducted on nail samples collected from 257 patients suspected of onychomycosis during 14 months. Fungal isolates were identified by polymerase chain reaction‐restriction fragment length polymorphisms (PCR‐RFLP) with the enzymes Msp I, Mva I, Alw I and sequencing. According to the results, out of the 257 patients participating in the study, onychomycosis was diagnosed in 180 (70.03%) cases, among which 51.1% were caused by non‐dermatophyte moulds (NDMs), 35% by yeast and 10.6% by dermatophytes. Numerous cryptic species recovered from onychomycosis for the first time. In the majority of cases, novel triazoles and imidazoles (ie, efinaconazole, luliconazole and lanoconazole) showed potent activity in comparison with other antifungal agents. The minimum inhibitory concentration (MIC) of luliconazole and lanoconazole ranged within 0.001 to >1 μg/mL and their geometric mean MICs were 0.0154 and 0.0309 μg/mL against all isolates, respectively. It seems that obtained data will be useful to improve the knowledge of researchers, clinicians and dermatologists about onychomycosis distribution, species diversity and adoption of appropriate treatment.

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Section: Discussionmentioning

confidence: 99%

Molecular identification and antifungal susceptibility of clinical fungal isolates from onychomycosis (uncommon and emerging species)

Haghani

Shams-Ghahfarokhi

Dalimi

et al. 2018

Mycoses

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“…Some investigators have reported T. rubrum as the most common etiological agent of ring worm in Tehran [9,31], while some others have found that T.…”

Section: Page 10 Of 18mentioning

confidence: 99%

A comparative study on morphological versus molecular identification of dermatophyte isolates

Ahmadi

Mirhendi

Shidfar

et al. 2015

Journal de Mycologie Médicale

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“…They play a significant role as pathogens in humans and animals (Summerbell et al, 1999;Cafarchia et al, 2013;Zarrin et al, 2015;Mochizuki et al, 2016). Concerning the recent epidemiologic surveys in Tehran, dermatophytosis was found to be 19.7%, and the major species involved the Trichophyton mentagrophytes/Trichophyton interdigitale species complex, Trichophyton rubrum, Trichophyton tonsurans, and Epidermophyton floccosum (Sadeghi et al, 2011;Zamani et al, 2016). It is believed that the PCR-based assays are quick and useful tools for the diagnosis and differentiation of isolates at the species and subspecies level, especially for the atypical isolates (Kac, 2000;Graser et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology, Genetic Diversity, and Antifungal Susceptibility of Major Pathogenic Dermatophytes Isolated From Human Dermatophytosis

2021

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BackgroundDermatophytes are a homogeneous group of species with low genetic diversity, and there are still many uncertainties about the boundaries among species.ObjectivesAiming at clarifying the relationships among species in the genus and introducing suitable genes for multilocus sequence typing (MLST), a new MLST scheme approach was developed to characterize the major pathogenic dermatophytes.MethodsWe performed maximum parsimony (MP), MrBayes, RAxML, and eBURST analyses, based on the MLST scheme to scrutinize the evolution within 95 clinical isolates and four reference strains belonging to the four major dermatophytes species. Then, the discriminatory power, pairwise genetic distances, ratio dN/dS, and sequence types (STs) of these isolates were determined. Also, to study taxonomy, sequences of the internal transcribed spacer (ITS), Beta-tubulin (BT2), and translation elongation factor 1-α (TEF-1α) genes of other dermatophytes species available in the GenBank were analyzed.ResultsFindings of the present study indicated that three genes: BT2, ITS, and TEF−1α, which showed the greatest diversity among dermatophyte species, were suitable for MLST. The most prevalent STs were seen among the species of Trichophyton interdigitale. Also, two new genotypes, i.e., XXVII and XXVIII, were introduced for T. interdigitale and Trichophyton mentagrophytes. The least informative sites were found in Epidermophyton floccosum, Trichophyton rubrum, and T. mentagrophytes, while the most informative sites were observed in T. interdigitale. Furthermore, the most informative locus was TEF-1α. The phylogenetic tree, constructed by the combination of the three genes, shows a new topological pattern that confirms the derivation of the anthropophilic and zoophilic genera from the geophilic genus. Also, the phylogenetic analyses and pairwise distances of the combination of the three loci showed that Trichophyton tonsurans and Trichophyton equinum were a species complex, where T. equinum is derived from T. tonsurans.ConclusionsResults of this study showed that MLST is very effective in determining the boundaries between species and taxonomy. Considering that there is no database for MLST dermatophytes, further studies are needed to determine the suitable genes for MLST. Also, the determination of STs in epidemiological studies and raising epidemiological information are helpful. This study was a new starting point to determine the ST and a foundation for a dermatophyte MLST database.

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A 4-year survey of dermatomycoses in Tehran from 2006 to 2009

Cited by 20 publications

References 26 publications

Molecular identification and antifungal susceptibility of clinical fungal isolates from onychomycosis (uncommon and emerging species)

Molecular identification and antifungal susceptibility of clinical fungal isolates from onychomycosis (uncommon and emerging species)

A comparative study on morphological versus molecular identification of dermatophyte isolates

Molecular Epidemiology, Genetic Diversity, and Antifungal Susceptibility of Major Pathogenic Dermatophytes Isolated From Human Dermatophytosis

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