A 5′UTR SNP of GHRHR locus is associated with body weight and average daily gain in Chinese cattle

Abstract

Growth hormone-releasing hormone receptor (GHRHR) has important functions in the regulation of the growth hormone axis and the development and proliferation of pituitary somatotropes. Moreover, some mutations in mouse GHRHR can induce the dwarfism. The objective of this paper is to reveal the association of GHRHR with growth traits in three Chinese cattle breeds, including Nanyang cattle (NY, 220), Qinchuan cattle (QC, 114), and Jiaxian cattle (JX, 142). A novel single nucleotide polymorphism (NM_181020:c.102C>T) in 5'UTR of GHRHR was identified using PCR-SSCP and DNA sequencing. The frequency of NM_181020:c.102C allele ranged from 0.926 to 0.956. We found that the locus was significantly associated with NY cattle's body weight (BW) of 6 months, with average daily gain (ADG) of 0-6 months, and as well as with ADG of 6-12 months (p < 0.05). The data suggested that the polymorphism (NM_181020:c.102C>T) of the GHRHR could be a molecular marker candidate for breeding of NY cattle in favor of BW.

Associations between genetic variants of the &lt;i&gt;POU1F1&lt;/i&gt; gene and production traits in Saanen goats

“…The relationships between genotypes and milk components were not found significant for POU1F1 / PstI locus. Similar to our results, Zhou et al (2016) reported that there was no significant relationship between POU1F1-PstI and milk performance in Guanzhong dairy goats. But they found that the TT genotype was higher than other genotypes for milk fat content and average milk fat.…”

“…This is because the POU1F1 transcription factor regulates the expression of genes GH, PRL, and TSHβ (Daga et al, 2013;Feng et al, 2012;Lan et al, 2009bLan et al, , 2007a. Research has been carried out to investigate the association of the POU1F1 gene with milk yield, milk composition, and growth traits in goats and cattle (Lan et al, 2007a, b;Zhang et al, 2009;Zhou et al, 2016). Some of the polymorphisms in the POU1F1 gene were reported to be related to growth, weaning weight, litter size and meat quality traits in sheep (Mura et al, 2012;Özmen et al, 2013;Sadeghi et al, 2014;Jalil-Sarghale et al, 2014;Bai et al, 2016; AL-Khuzai and AL-Anbari 2018), milk production in cattle (Ahmadi et al, 2015), carcass weight in cattle (Seong et al, 2011), meat quality in rabbits (Wang et al, 2015), and milk production, growth traits and litter size in goats (Daga et al, 2013;Feng et al, 2012;Ma et al, 2017).…”

Polymorphisms of the Growth Hormone Releasing Hormone Receptor Gene Affect Body Conformation Traits in Chinese Dabieshan Cattle

“…The GHRHR was reported to encode the GHRH receptor, and its activation would release the GH stored in secretory granules and produce GH through cAMP-dependent GH1 transcription [25]. Previous studies found that the GHRHR plays a pivotal role in complex biological process, especially multiple inflammatory processes [26], cell proliferation and development [27], and body conformation traits [12][13][14]. In this study, the GT-AG rule was appropriate for the GHRHR in bovines, indicating that it conforms to the splice site of the eukaryotic gene.…”

“…In Lanzhou fat-tail sheep population, a GHRHR deletion mutation caused significant body weight loss [12], and the mutations in the GHRHR significantly affect body length (BL) and body height (BH) of the Guanzhong goat and Xinong Sannen dairy goats [13]. In Nanyang cattle, a mutation in 5 UTR of the GHRHR significantly influenced the body weight (BW)at6 months, and average daily gain (ADG) at 0-6 months and 6-12 months (p < 0.05) [14]. These results suggested that the GHRHR plays a vital role in the growth and development of livestock.…”

Yak Sığırında SIRT1 ve H-FABP Genlerinde Belirlenen Tek Nükleotid Polimorfizmleri ve Büyüme Özellikleri İle İlişkisi

“…A SNP within 5'UTR region of zinc finger, BEDtype containing 6 (ZBED6) gene resulted in transcription factor change, and then altered the transcription activity and mRNA expression in beef cattle [29] . A 5'UTR-region SNP of growth hormone-releasing hormone receptor (GHRHR) gene locus had been found to be associated with body weight and average daily gain in Chinese cattle [30] . Thus, it was an interesting work to find out the mechanism for the association between these 5'UTR-region SNPs and the growth traits in yak.…”