A 50-Hz magnetic-field exposure promotes human amniotic cells proliferation via SphK–S1P–S1PR cascade mediated ERK signaling pathway

Chen, Liangjing; Xia, Yong-Peng; Lu, Jingchun; Xie, Qixin; Ye, Anfang; Sun, Wenjun

doi:10.1016/j.ecoenv.2020.110407

Cited by 12 publications

(5 citation statements)

References 36 publications

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“…In a study of depression, it was found that rTMS promoted the proliferation of hippocampal neural stem cells by increasing the level of p-ERK1/2 [30]. In addition, magnetic stimulation promoted the proliferation of human amniotic membrane cells by activating the ERK1/2 pathway [31]. Tumor necrosis factorconvertase (TACE) is the key enzyme for p75ECD release, and its activation is achieved through phosphorylation modification.…”

Section: Discussionmentioning

confidence: 99%

Repetitive Transcranial Magnetic Stimulation Decreases Serum Amyloid-β and Increases Ectodomain of p75 Neurotrophin Receptor in Patients with Alzheimer’s Disease

Tao¹,

Liu²,

Zhu³

et al. 2022

J. Integr. Neurosci.

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Background: This study investigated the impact of repetitive transcranial magnetic stimulation (rTMS) on serum levels of Amyloid-β (Aβ) as well as the ectodomain of p75 neurotrophin receptor (p75ECD) in patients with Alzheimer's disease (AD). Methods: A total of 46 patients diagnosed with AD between June 1, 2020 and December 31, 2021 were randomized to undergo either 20 Hz rTMS treatment of the left dorsolateral prefrontal cortex (DLPFC) or sham procedure. Cognitive function and activity of daily living were evaluated. Neuropsychological tests and blood samples were gathered at baseline and at 2, 3, 4, and 6 weeks after rTMS therapy. Results: There were no evident differences between rTMS group and sham group in serum Aβ40, Aβ42, total Aβ, ApoE, and p75ECD standards at baseline (p > 0.05). Serum levels of Aβ40, Aβ42, as well as total Aβ, were significantly lower in the rTMS group at 3, 4 and 6 weeks relative to the sham group (p < 0.05). Serum p75ECD levels in the rTMS group were significantly higher than those of the sham group at 3, 4 and 6 weeks (p < 0.05). Levels of serum Aβ40 (r: -0.78, -0.83, -0.68, respectively), Aβ42 (r: -0.76, -0.76, -0.61, respectively) and total Aβ (r: -0.74, -0.81, -0.66, respectively) were negatively correlated with MoCA, MMSE and MBI scores, while serum p75ECD levels (r: 0.84, 0.90, 0.72, respectively) were positively correlated (p < 0.01). The level of serum Aβ40 (r = 0.77), Aβ42 (r = 0.69) as well as total Aβ (r = 0.73) were positively correlated with ADAS-cog score, while p75ECD levels (r = -0.86) were negatively correlated (p < 0.01). Conclusions: The results of this study suggest that rTMS may decrease serum Aβ levels and increase serum p75ECD levels in patients with AD, offering insight into a potential underpinning mechanism of rTMS.

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Section: Discussionmentioning

confidence: 99%

Repetitive Transcranial Magnetic Stimulation Decreases Serum Amyloid-β and Increases Ectodomain of p75 Neurotrophin Receptor in Patients with Alzheimer’s Disease

Tao¹,

Liu²,

Zhu³

et al. 2022

J. Integr. Neurosci.

View full text Add to dashboard Cite

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“…Further studies will be needed to test this possibility and to decipher the mechanisms involved. IR can also simultaneously induce multiple signaling pathways that promote cell survival, such as AKT, ATM/ATR, and ERK cascades (56). It is well-known that the pro-survival signaling pathways generally lead to suppression of apoptosis, activation of cell cycle checkpoint and initiation of DNA repair in cancer cells.…”

Section: Discussionmentioning

confidence: 99%

RAC1 Involves in the Radioresistance by Mediating Epithelial-Mesenchymal Transition in Lung Cancer

Tan

Wang

et al. 2020

Front. Oncol.

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Radiation therapy is a common and acceptable approach for lung cancer. Although the benefit of ionizing radiation (IR) is well-established, cancer cells can still survive via pro-survival and metastasis signaling pathways. Ras related C3 botulinum toxin substrate1 (RAC1), a member of Rho family GTPases, plays important roles in cell migration and survival. In the present study, we investigated the effects of RAC1 on the survival of lung cancer cells treated with irradiation. The results showed RAC1 is overexpressed in lung cancer cells and promoted cell proliferation and survival. Furthermore, IR induced RAC1 expression and activity via the activation of PI3K/AKT signaling pathway, and then enhancing cell proliferation, survival, migration and metastasis and increasing levels of epithelial-to-mesenchymal transition (EMT) markers, which facilitated the cell survival and invasive phenotypes. In addition, overexpression of RAC1 attenuated the efficacy of irradiation, while inhibition of RAC1 enhanced sensitivity of irradiation in xenograft tumors in vivo. Collectively, we further found that RAC1 enhanced radioresistance by promoting EMT via targeting the PAK1-LIMK1-Cofilins signaling in lung cancer. Our finding provides the evidences to explore RAC1 as a therapeutic target for radioresistant lung cancer cells.

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“…S1PR3 is more highly expressed in the synovium of arthritis (Inoue et al, 2019). S1PR1/3 is mainly involved in the inflammatory response, promoting cells angiogenesis, differentiation and proliferation (Hsu et al, 2015;Chen et al, 2020). S1P can stimulate Gαi and Gαs protein-dependent signaling expression and regulate the level of cAMP expression (Lagadari et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Effect of Geniposide on Regulating the Functions of Rheumatoid Arthritis Synovial Fibroblasts via Inhibiting Sphingosine-1-Phosphate Receptors1/3 Coupling Gαi/Gαs Conversion

et al. 2020

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The activated Gα protein subunit (Gαs) and the inhibitory Gα protein subunit (Gαi) are involved in the signal transduction of G protein coupled receptors (GPCRs). Moreover, the conversion of Gαi/Gαs can couple with sphingosine-1-phosphate receptors (S1PRs) and have a critical role in rheumatoid arthritis (RA). Through binding to S1PRs, sphingosine-1-phosphate (S1P) leads to activation of the pro-inflammatory signaling in rheumatoid arthritis synovial fibroblasts (RASFs). Geniposide (GE) can alleviate RASFs dysfunctions to against RA. However, its underlying mechanism of action in RA has not been elucidated so far. This study aimed to investigate whether GE could regulate the biological functions of MH7A cells by inhibiting S1PR1/3 coupling Gαi/Gαs conversion. We use RASFs cell line, namely MH7A cells, which were obtained from the patient with RA and considered to be the main effector cells in RA. The cells were stimulated with S1P (5 μmol/L) and then were treated with or without different inhibitors: Gαi inhibitor pertussis toxin (0.1 μg/mL), S1PR1/3 inhibitor VPC 23019 (5 μmol/L), Gαs activator cholera toxin (1 μg/mL) and GE (25, 50, and 100 μmol/L) for 24 h. The results showed that GE may inhibit the abnormal proliferation, migration and invasion by inhibiting the S1P-S1PR1/3 signaling pathway and activating Gαs or inhibiting Gαi protein in MH7A cells. Additionally, GE could inhibit the release of inflammatory factors and suppress the expression of cAMP, which is the key factor of the conversion of Gαi and Gαs. GE could also restore the dynamic balance of Gαi and Gαs by suppressing S1PR1/3 and inhibiting Gαi/Gαs conversion, in a manner, we demonstrated that GE inhibited the activation of Gα downstream ERK protein as well. Taken together, our results indicated that down-regulation of S1PR1/3-Gαi/Gαs conversion may play a critical role in the effects of GE on RA and GE could be an effective therapeutic agent for RA.

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A 50-Hz magnetic-field exposure promotes human amniotic cells proliferation via SphK–S1P–S1PR cascade mediated ERK signaling pathway

Cited by 12 publications

References 36 publications

Repetitive Transcranial Magnetic Stimulation Decreases Serum Amyloid-β and Increases Ectodomain of p75 Neurotrophin Receptor in Patients with Alzheimer’s Disease

Repetitive Transcranial Magnetic Stimulation Decreases Serum Amyloid-β and Increases Ectodomain of p75 Neurotrophin Receptor in Patients with Alzheimer’s Disease

RAC1 Involves in the Radioresistance by Mediating Epithelial-Mesenchymal Transition in Lung Cancer

Anti-Inflammatory Effect of Geniposide on Regulating the Functions of Rheumatoid Arthritis Synovial Fibroblasts via Inhibiting Sphingosine-1-Phosphate Receptors1/3 Coupling Gαi/Gαs Conversion

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