A 50‐Hz magnetic field induces structural and biophysical changes in membranes

Paradisi, Silvia; Donelli, Gianfranco; Santini, Maria Teresa; Straface, Elisabetta; Malorni, Walter

doi:10.1002/bem.2250140308

Cited by 97 publications

(61 citation statements)

References 23 publications

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“…Cambios semejantes han sido reportados con el uso de diversos campos magnéticos, pulsantes o constantes, en cultivos celulares tanto en células adherentes como no adherentes (8,9,10,11,12). En nuestro estudio, sin embargo, no se apreciaron cambios morfológicos en las células no adherentes K-562 (eritroleucemia humana) Los cambios que han sido descritos por la exposición a campos magnéticos moderados (6mT) consisten, para el caso de células que crecen en suspensión, en la pérdida de su forma redondeada y en la adquisición de una alargada e irregular, mientras que para las células adherentes se trata de modificaciones en su forma y orientación o en la pérdida de su adherencia, quedando, de esta manera, libremente suspendidas en el cultivo (13).…”

Section: Discussionunclassified

“…Sin embargo, otras investigaciones que han obtenido resultados semejantes explican esta respuesta, exclusivamente, por un aumento de la apoptosis de las células tumorales (16), puesto que en ningún caso informado hasta ahora, se ha encontrado necrosis como el mecanismo involucrado en la reducción de la viabilidad celular (9,17) Varias hipótesis pretenden explicar el origen de la apoptosis inducida por los campos magnéticos pulsantes. Han sido implicados el arresto del ciclo celular, la activación de Caspasa 3, el aumento en las EROs (14) la activación de genes promotores de proteínas de choque térmico (18) y la activación de vías de señalización relacionadas con apoptosis.…”

Section: Discussionunclassified

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Efecto De Los Campos Magnéticos Pulsantes Sobre Líneas De Células Tumorales McF-7 Y K-562

Pinilla¹,

Pombo²,

Urueña³

et al. 2014

rev cuarzo

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RESUMENNumerosas investigaciones sugieren que los campos magné-ticos inducen apoptosis en diversas líneas tumorales, aparentemente sin afectar a células sanas. Sin embargo, las conclusiones son heterogéneas debido a las diferencias en las características de los campos magnéticos utilizados. Nuestro estudio se realizó con el objetivo de determinar el efecto de los campos magnéticos pulsantes sobre la morfología, el recuento celular y la viabilidad en líneas tumorales MCF-7 y K-562. Las células fueron expuestas a campos pulsantes positivos , de 6 mT y de 60 Hz, por periodos de una o de tres horas . El efecto se comparó con el producido por Doxorrubicina 0,075 µM o 10 µM. En este artículo se exponen los principales resultados de dicho estudio.Palabras clave: cáncer, medicina alternativa, terapéuticas alternativas, viabilidad celular, apoptosis, campos magnéticos pulsantes. ABSTRACTNumerous studies suggest that magnetic fields induce apoptosis in various tumor cell lines, apparently without affecting healthy cells. However, the conclusions are heterogeneous due to the differences in the characteristics of magnetic fields used. Our study was conducted to determine the effect of pulsed magnetic fields on the morphology, cell count and viability in tumor cell lines MCF-7 and K-562. Cells were exposed to positive pulsed field of 6 mT and 60 Hz, for periods of one or three hours. The effect was compared with that produced by doxorubicin 0.075 µM to 10 µM. This article presents the main results of the study are presented.

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Section: Discussionunclassified

Efecto De Los Campos Magnéticos Pulsantes Sobre Líneas De Células Tumorales McF-7 Y K-562

Pinilla¹,

Pombo²,

Urueña³

et al. 2014

rev cuarzo

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“…The phosphorylation of pro-caspase 9 is mediated by PI3 Kinase/ Akt pathway [51]. Possibly, the biophysical changes of cell membrane in the presence of 50 Hz EMF [52] might increase the August 2012 | Issue 2 | 2 Figure 6. Proposed hypothetical mechanism of inhibition of apoptosis by repression of caspase 9 in primary lymphocytes exposed to 50 Hz ELF-EMF based on the present experimental investigation and contemporary literature [51,52,58].…”

Section: Discussionmentioning

confidence: 99%

“…Possibly, the biophysical changes of cell membrane in the presence of 50 Hz EMF [52] might increase the August 2012 | Issue 2 | 2 Figure 6. Proposed hypothetical mechanism of inhibition of apoptosis by repression of caspase 9 in primary lymphocytes exposed to 50 Hz ELF-EMF based on the present experimental investigation and contemporary literature [51,52,58]. The biophysical changes of cell membrane in presence of 50 Hz EMF might increase the cytosolic Ca 2+ concentration leading to the activation of PI3 kinase leading to the repression of apoptotic pathway initiated by pro-caspase 9.…”

Section: Discussionmentioning

confidence: 99%

Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity

Rajendra¹,

Sujatha²,

Sashidhar³

et al. 2012

Biodiscovery

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The cell viability and DNA damage in unstimulated sheep primary lymphocytes subjected to different extremely low electromagnetic field intensities (5, 50 and 100 µT; 50 Hz) were studied with special emphasis on apoptosis. Sheep primary lymphocytes cultured in RPMI, supplemented with 10% FBS in the absence of mitogens, were exposed till 16 h. The cell viability assessment by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay showed a dose dependent enhancement of viability at 16 h. Further, quantitative DNA laddering and flow cytometric analysis showed a significant decrease in apoptosis of the cells subjected to 100 (p<0.01) and 50 µT (p<0.05) for 16 h as compared to control group. There was a statistically significant decrease (p<0.01) in the specific activity of caspase 9 at 100 µT in cells exposed for 16 h. However, no enhancement of DNA damage was observed at 5, 50 and 100 µT as evidenced by comet assay. Comet assay also confirmed the decreased cell death of exposed cells (100 µT). Experimental data suggests decreased apoptosis at 100 µT (50 Hz), possibly by the suppression of caspase 9 activity leading to the enhanced cell viability. Effect of ELF-EMF on primary lymphocytesBioDiscovery | www.biodiscoveryjournal.co.uk

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“…But there is a supposition that the most sensitive element to the magnetic field action a cell plasmolemma due to its bioelectric properties [18,19]. It has been reported previously that SMF of medium intensity can change plasmolemma functions in different types of cells [20][21][22][23][24][25]. One of the most important functional characteristic is its ability to adhesion.…”

Section: Magnetic Field Influence On Cells Adhesion Morphology and Pmentioning

confidence: 99%

Perspectives of Constant Gradient Magnetic Fields Applications in Biotechnology

Ignatyeva¹,

Voyevodin²,

Goltsev³

et al. 2014

BIO

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Elastic hard magnetic materials based resin-bond magnets with the determined space configuration of the magnetic field required for a three-dimensional cell growth which is essential for the tissue engineering have been produced. Technical tests of the samples as well as the theoretical study of the distribution of stray fields produced by ferromagnetic particles correspondingly distributed in the film have been carried out. In vitro еxperimental investigations of the gradient magnetic field influence on a cell differentiation on transplanted epithelial-like kidney cells culture of a pig embryo has been carried out. It has been shown that the adhesion, morphology and proliferation rate of the cells is determined not only by the magnetic field value but also by its gradient direction. It has been established that the cell adhesion efficiency is the highest when the magnetic field gradient is directed from the Petri dish bottom to the air-culture medium interface. The obtained results prove the possibility of an implementation of new gradient magnetic fields based methods in biotechnology and in particular in tissue engineering.

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A 50‐Hz magnetic field induces structural and biophysical changes in membranes

Cited by 97 publications

References 23 publications

Efecto De Los Campos Magnéticos Pulsantes Sobre Líneas De Células Tumorales McF-7 Y K-562

Efecto De Los Campos Magnéticos Pulsantes Sobre Líneas De Células Tumorales McF-7 Y K-562

Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity

Perspectives of Constant Gradient Magnetic Fields Applications in Biotechnology

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