A 9-cis-epoxycarotenoid dioxygenase inhibitor for use in the elucidation of abscisic acid action mechanisms

Kitahata, Nobutaka; Han, Sun Young; Noji, Natsumi; Saito, Tamio; Kobayashi, Masatomo; Nakano, Takeshi; Kuchitsu, Kazuyuki; Shinozaki, Kazuo; Yoshida, Shigeo; Matsumoto, Shogo; Tsujimoto, Masafumi; Asami, Tadao

doi:10.1016/j.bmc.2006.04.025

Cited by 68 publications

(54 citation statements)

References 34 publications

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“…In addition, fruit and leaves from these three cultivars were sprayed with abamine, which is a competitive inhibitor of 9-cis-epoxycarotenoid dioxygenase (Kitahata et al, 2006), at a concentration of 100 mM to inhibit ABA biosynthesis in fruit flesh. These treatments started from the preflowering developmental stage and continued until the midfruit stage, at which PA accumulation in A-type cultivars was terminated (Akagi et al, 2009b).…”

Section: Effects Of Aba and Its Biosynthesis Inhibitor On Pa Accumulamentioning

confidence: 99%

“…This fertilizer was sprayed at a concentration of 4,000 mL L 21 (400 mL L 21 [approximately 1.5 mM] S-ABA). In addition, fruits and leaves of these three cultivars were sprayed with abamine, which is a competitive inhibitor of 9-cis-epoxycarotenoid dioxygenase (Kitahata et al, 2006), at a concentration of 100 mM to inhibit ABA biosynthesis in fruit flesh. These treatments were performed at 3-d intervals throughout the experiment, starting at 1 WAB, in the experimental orchard of Kyoto University.…”

Section: Application Of Aba To Persimmon Fruit In Plantamentioning

confidence: 99%

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Seasonal Abscisic Acid Signal and a Basic Leucine Zipper Transcription Factor, DkbZIP5, Regulate Proanthocyanidin Biosynthesis in Persimmon Fruit

et al. 2011

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Proanthocyanidins (PAs) are secondary metabolites that contribute to plant protection and crop quality. Persimmon (Diospyros kaki) has a unique characteristic of accumulating large amounts of PAs, particularly in its fruit. Normal astringent-type and mutant nonastringent-type fruits show different PA accumulation patterns depending on the seasonal expression patterns of DkMyb4, which is a Myb transcription factor (TF) regulating many PA pathway genes in persimmon. In this study, attempts were made to identify the factors involved in DkMyb4 expression and the resultant PA accumulation in persimmon fruit. Treatment with abscisic acid (ABA) and an ABA biosynthesis inhibitor resulted in differential changes in the expression patterns of DkMyb4 and PA biosynthesis in astringent-type and nonastringent-type fruits depending on the development stage. To obtain an ABA-signaling TF, we isolated a full-length basic leucine zipper (bZIP) TF, DkbZIP5, which is highly expressed in persimmon fruit. We also showed that ectopic DkbZIP5 overexpression in persimmon calluses induced the up-regulation of DkMyb4 and the resultant PA biosynthesis. In addition, a detailed molecular characterization using the electrophoretic mobility shift assay and transient reporter assay indicated that DkbZIP5 recognized ABA-responsive elements in the promoter region of DkMyb4 and acted as a direct regulator of DkMyb4 in an ABA-dependent manner. These results suggest that ABA signals may be involved in PA biosynthesis in persimmon fruit via DkMyb4 activation by DkbZIP5.

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Section: Effects Of Aba and Its Biosynthesis Inhibitor On Pa Accumulamentioning

confidence: 99%

Section: Application Of Aba To Persimmon Fruit In Plantamentioning

confidence: 99%

Seasonal Abscisic Acid Signal and a Basic Leucine Zipper Transcription Factor, DkbZIP5, Regulate Proanthocyanidin Biosynthesis in Persimmon Fruit

et al. 2011

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“…These results suggest that chilling seeds at 5°C for 2 weeks was not enough to avoid dwarfism and lesions in this study. On the other hand, fluridone, a type of herbicide, inhibited all carotenoids and carotenoid-derived metabolites and could cause plants lethal damage because carotenoids play an important role in protecting photosynthetic organisms against damage by photooxidation and absorb light energy in plants (Kitahata et al, 2006). Therefore, fluridone could influence lesion syndrome even in treated seeds in this study.…”

Section: Id_genesmentioning

confidence: 72%

Effects of Fluridone Treatment on Seed Germination and Dormancy-associated Gene Expression in an Ornamental Peach (<i>Prunus persica</i> (L.) Batsch)

et al. 2017

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Fluridone, an inhibitor of carotenoid biosynthesis, is known to prevent abscisic acid (ABA) biosynthesis and to affect the germination and dormancy of seeds in several plant species. This study investigated the effects of fluridone on seed germination of the ornamental peach 'Yaguchi', and on the transcript levels of genes related to seed dormancy in this plant. Seeds were rinsed for 2 days under running tap water (RS), then soaked for 1 day in 100 μM fluridone (F), and kept at 5°C for 2 weeks (2W). The germination rate significantly increased from 0% to 9.5% in the RS+F treatment and to 71.4% in the RS+F+2W treatment, while no germination occurred in the RS and RS+2W treatments. Seedlings in RS+F and RS+F+2W treatments formed dwarf shoots, i.e. about 10 cm, and morphological lesions on the leaves. The ABA content in embryonic axes decreased after RS and increased with RS+2W. It was decreased by the RS+F+2W treatment. RS+F+2W downregulated ABA-hy3, which encodes enzymes with key roles in ABA catabolism, while its effects on 9-cisepoxycarotenoid dioxygenase (NCED1) in relation to ABA synthesis fluctuated. In contrast, Empfindlicherim Dunkelroten Licht 1 (EID1) was upregulated after fluridone treatment, suggesting that fluridone may activate positive ABA signaling pathways. Expression of GA2-oxidase8 (GA2-ox8) was not affected by fluridone. D-34) were downregulated in the RS+F+2W treatment, suggesting that the expression of these genes are controlled by low temperature and the ABA inhibitor, and are involved in seed dormancy. These results suggest that ABA inhibitor treatments can be an alternative method to promote germination by controlling ABA content and its metabolism, and consequently changing expression of certain ABA-and dormancy-related genes including ABA-hy3, EID1, LeMADS, and LEA D-34, even under insufficient chilling conditions. MADS-box protein JOINTLESS (LeMADS) and Late embryogenesis abundant protein D-34 (LEA

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“…17) Abamine (ABM) is the first reported abscisic acid biosynthesis inhibitor that targets nine-cis-epoxycarotenoid dioxygenases (NCED), 12,13) and it has been suggested that abamine may also target other CCDs. 18) Interestingly, AbamineSG (ABMSG) was developed as an abscisic acid biosynthesis inhibitor based on the structural and functional characteristics of abamine, but was found to be a more specific and potent NCED inhibitor than abamine. 18) Recently Ropez-Reaz et al reported that abamineSG reduced the level of strigolactone in tomato plants and concluded that this reduction may be due to the inhibition of abscisic acid biosynthesis; 19) however, the potency of abamine for the regulation of strigolactone production is yet to be determined.…”

Section: Resultsmentioning

confidence: 99%

Abamine as a basis for new designs of regulators of strigolactone production

et al. 2011

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Nine-cis-epoxycarotenoid dioxygenase (NCED), belonging to the family of carotenoid-cleaving dioxygenases (CCDs), is the ratelimiting step in the abscisic acid (ABA) biosynthetic pathway in plants. Abamine is the first reported abscisic acid biosynthesis inhibitor to target NCED. Its utility has also been considered in the inhibition of other enzymes. As CCDs other than NCED are also involved in the strigolactone biosynthetic pathway, abamine may affect strigolactone biosynthesis. In this study, we found that abamine decreases the level of strigolactones in several plant species and reduces the germination rate of Orobanche minor seeds co-grown with tobacco. Taken together, these data suggest that abamine may be used as a scaffold for the development of specific regulators of strigolactone production.

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A 9-cis-epoxycarotenoid dioxygenase inhibitor for use in the elucidation of abscisic acid action mechanisms

Cited by 68 publications

References 34 publications

Seasonal Abscisic Acid Signal and a Basic Leucine Zipper Transcription Factor, DkbZIP5, Regulate Proanthocyanidin Biosynthesis in Persimmon Fruit

Seasonal Abscisic Acid Signal and a Basic Leucine Zipper Transcription Factor, DkbZIP5, Regulate Proanthocyanidin Biosynthesis in Persimmon Fruit

Effects of Fluridone Treatment on Seed Germination and Dormancy-associated Gene Expression in an Ornamental Peach (<i>Prunus persica</i> (L.) Batsch)

Abamine as a basis for new designs of regulators of strigolactone production

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