A 90-day safety study of genetically modified rice expressing Cry1Ab protein (Bacillus thuringiensis toxin) in Wistar rats

Schrøder, Malene; Poulsen, Morten; Wilcks, Andrea; Kroghsbo, Stine; Miller, Andreas; Frenzel, Thomas; Danier, Jürgen; Rychlik, Michael; Emami, Kaveh; Gatehouse, A. M. R.; Shu, Qingyao; Engel, Karl‐Heinz; Altosaar, Illimar; Knudsen, Ib

doi:10.1016/j.fct.2006.09.001

Cited by 140 publications

(119 citation statements)

References 34 publications

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“…However, in these studies, mycotoxin contamination was not investigated and it has been shown that mycotoxins could lead to liver damage, and thus reduced serum TP (Casteel et al, 1993). Similar to findings from the present study, the lower serum TP observed by Schrøder et al (2007) in rats did not occur in conjunction with histological or organ weight changes, thus questioning the biological relevance of this alteration.…”

Section: Discussionsupporting

confidence: 64%

“…Numerous studies have reported a lack of an effect of GM maize on blood biochemistry in weanling pigs (Walsh et al, 2011), calves (Shimada et al, 2006) and rats (Hammond et al, 2006) or on serum TP in sheep (Trabalza-Marinucci et al, 2008). A decrease in serum TP was described in rats fed GM maize for three generations (Kilic and Akay, 2008) and in rats fed GM rice (Schrøder et al, 2007). Kilic and Akay (2008) found that serum TP of rats fed GM maize was lower only compared with rats fed non-GM maize but was similar to that of rats fed a commercial control diet.…”

Section: Discussionmentioning

confidence: 99%

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Effect of feeding genetically modified Bt MON810 maize to ∼40-day-old pigs for 110 days on growth and health indicators

Buzoianu

Walsh

Rea

et al. 2012

animal

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A total of 72 male weaned pigs were used in a 110-day study to investigate the effect of feeding genetically modified (GM) Bt MON810 maize on selected growth and health indicators. It was hypothesised that in pigs fed Bt maize, growth and health are not impacted compared with pigs fed isogenic maize-based diets. Following a 12-day basal period, pigs (10.7 ± 1.9 kg body weight (BW); ∼40 days old) were blocked by weight and ancestry and randomly assigned to treatments: (1) non-GM maize diet for 110 days (non-GM), (2) GM maize diet for 110 days (GM), (3) non-GM maize diet for 30 days followed by GM maize diet up to day 110 (non-GM/GM) and (4) GM maize diet for 30 days followed by non-GM maize diet up to day 110 (GM/non-GM). BW and daily feed intake were recorded on days 0, 30, 60 and 110 (n= 15). Body composition was determined by dual energy X-ray absorptiometry (n= 10) on day 80. Following slaughter on day 110, organs and intestines were weighed and sampled for histological analysis and urine was collected for biochemical analysis (n= 10). Serum biochemistry analysis was performed on days 0, 30, 60, 100 and 110. Growth performance and serum biochemistry were analysed as repeated measures with time and treatment as main factors. Thesliceoption of SAS was used to determine treatment differences at individual time points. There was no effect of feeding GM maize on overall growth, body composition, organ and intestinal weight and histology or serum biochemistry on days 60 and 100 and on urine biochemistry on day 110. A treatment × time interaction was observed for serum urea (SU;P< 0.05), creatinine (SC;P< 0.05) and aspartate aminotransferase (AST;P< 0.05). On day 30, SU was lower for the non-GM/GM treatment compared with the non-GM, GM and GM/non-GM treatments (P< 0.05). On day 110, SC was higher for the non-GM/GM and GM/non-GM treatments compared with non-GM and GM treatments (P< 0.05). Overall, serum total protein was lower for the GM/non-GM treatment compared with the non-GM/GM treatment (P< 0.05). The magnitude of change observed in some serum biochemical parameters did not indicate organ dysfunction and the changes were not accompanied by histological lesions. Long-term feeding of GM maize to pigs did not adversely affect growth or the selected health indicators investigated.

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Effect of feeding genetically modified Bt MON810 maize to ∼40-day-old pigs for 110 days on growth and health indicators

Buzoianu

Walsh

Rea

et al. 2012

animal

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“…Hammond et al found that overall health, body weight gain, food consumption, clinical pathology parameters (hematology, blood chemistry, urinalysis), organ weights, and gross and microscopic appearance of tissues were comparable between rats fed Bt and conventional corn varieties for 90 days 26 . Furthermore, some research groups also reported similar results in their 90-day safety studies using rats 32,33,43 . In addition to these general toxicity tests, a special toxicity test has been published.…”

Section: Small Experiments Animalssupporting

confidence: 53%

Safety Evaluation of Bt Plants

Shimada

Murata

Miyazaki

2008

JARQ

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Bt plants are insect-resistant plants engineered to carry the gene coding the insecticidal protein of a soil bacterium, Bacillus thuringiensis. These transgenic plants provide a highly effective method of insect control and reduce dependence on pesticide chemicals. In consequence, they have provided higher yields, reduced crop mycotoxin levels because of the effective lowering of the insect damage, and have decreased the pesticide spraying work and cost. However, there are public concerns about the safety of Bt plants for humans, animals and the environment. This review describes recent research on the safety evaluation of Bt plants, focusing on our results in the toxicological studies of Bt11 corn.

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“…The leukocyte count was also not altered by the GM material occurring in feeds. Similarly, in the experiment with GM rice (expressing Cry1Ab protein) offered to rats, the haematological indices were not affected by the treatment (17).…”

Section: Discussionmentioning

confidence: 83%

Effect of feeding genetically modified maize and soybean meal to sows on their reproductive traits, haematological indices and offspring performance

Świątkiewicz

Bednarek

Markowski

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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The effect of Roundup Ready MON-40-3-2 soybean meal and Bt maize MON810 on sows performance and haematological indices, including parameters of erythrocytes, leukocytes, and trombocytes, as well as the piglets rearing indices were evaluated in the experiment carried out on 24 sows and their progeny. After mating sows were divided into groups: I -control, conventional soybean meal and conventional maize; II -genetically modified (GM) soybean meal and conventional maize; IIIconventional soybean meal and GM maize; IV -GM soybean meal and GM maize. All used feed mixtures were isonitrogenous and isoenergetic, however differed in presence or absence of genetically modified maize MON810 (5% for pregnant and 8% for lactating sows) and/or soybean meal MON-40-3-2 (4% for pregnant and 14% for lactating sows). Born piglets were allotted to the same group as their mothers. The study showed that feeding pregnant and lactating sows with mixtures containing genetically modified RR soybean or/and meal Bt maize did not significantly affect their reproductive characteristics and offspring performance. There was no effect of dietary treatment on haematological indices. Transfer of transgenic DNA from GM feed to blood was not confirmed.

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A 90-day safety study of genetically modified rice expressing Cry1Ab protein (Bacillus thuringiensis toxin) in Wistar rats

Cited by 140 publications

References 34 publications

Effect of feeding genetically modified Bt MON810 maize to ∼40-day-old pigs for 110 days on growth and health indicators

Effect of feeding genetically modified Bt MON810 maize to ∼40-day-old pigs for 110 days on growth and health indicators

Safety Evaluation of Bt Plants

Effect of feeding genetically modified maize and soybean meal to sows on their reproductive traits, haematological indices and offspring performance

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