A 96-well Plate Based Dot-ELISA Array for Simultaneous Detection of Multi-Drugs

Wang, Shuang; Wei, Yibin; Jin, Haiming; Chuan-bao, LI; Du, Hongwu

doi:10.1080/00032710903202030

Cited by 10 publications

(4 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Visualization assays based on membranes, such as the lateral flow assay (LFA) and dot ELISA, have been widely used in clinical diagnosis, food safety, and environmental analysis. − ,, Antibodies or antigens are immobilized on NC or polyvinylidene fluoride (PVDF) membranes, and the color of a line or a dot is developed by different molecular signal generators, such as gold nanoparticles, latex particles, or an enzyme. In our experimental design, MWCNTs were used as a solid support for immobilization of the antibody.…”

Section: Resultsmentioning

confidence: 99%

“…When the antibody attaches to the planar surface by adsorption, the avidity of the antibody is lowered and, consequently, more time is required to achieve the antibody–antigen binding equilibrium; this can influence the performance of assays, such as sensitivity. Recently, a new generation of materials has been adopted as a solid support in immunoassays, including magnetic nanoparticles (MNPs), − metal nanoparticles, − and carbon nanotubes (CNTs). − …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Use of Carbon Nanotubes as a Solid Support To Establish Quantitative (Centrifugation) and Qualitative (Filtration) Immunoassays To Detect Gentamicin Contamination in Commercial Milk

Zeng

Wei

Jiang

et al. 2016

J. Agric. Food Chem.

View full text Add to dashboard Cite

Current methods to detect gentamicin (GEN), a broad-spectrum antibiotic that causes ototoxicity and nephrotoxicity when present in excess, have several limitations. Hence, we have developed two methods using multi-walled carbon nanotubes (MWCNTs) as a solid support to detect GEN. Hybridoma cells (2D12) producing high-sensitivity antibodies against GEN were established. The goat anti-mouse antibody was immobilized on MWCNTs directly or using bifunctional polyethylene glycol as a linker. On the basis of the physical characteristics of MWCNTs, a quantitative method involving centrifugation separation and a qualitative method involving filtration separation were established. Various experimental parameters were optimized for GEN detection, and recovery tests were performed. For the quantitative method, the limit of detection (LOD) was 0.048 ng/mL, whereas for the qualitative method, a LOD of 0.1 ng/mL was observed by the naked eye. The proposed immunoassays were applied to commercial milk samples. Thus, these methods show potential application for the detection of GEN.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Use of Carbon Nanotubes as a Solid Support To Establish Quantitative (Centrifugation) and Qualitative (Filtration) Immunoassays To Detect Gentamicin Contamination in Commercial Milk

Zeng

Wei

Jiang

et al. 2016

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…The Multi-Dot-ELISA method in this study is a modified non-instrument-dependent ELISA technique, the character of high-throughput makes it possible to test a lot of samples simultaneously [15,16]. Moreover, this method will be more suitable for the detection of antibiotics, which cooperate with our previous study to provide a basis for the analysis of other antigen using this method [17][18][19].…”

Section: Introductionmentioning

confidence: 82%

Development of a High-Throughput Immunoassay for Rapid Detection of Multiple Antibiotic Residues in Cosmetics

Xun

Yan

Liu

et al. 2014

AMR

View full text Add to dashboard Cite

Antibiotic residues in cosmetics can cause seriously healthy problems such as sensitization, systemic toxicity, or even death. The developments of rapid methods for multiple antibiotics detection are needed to conveniently detect out the illegal additives. In this study, a simple, cost-effective and time-saving immunoassay, multi-analyte dot enzyme-linked immunosorbent assay (Multi-Dot-ELISA), for the simultaneous detection of three most common abused antibiotics (CPFX, TC, and SMD) was successfully designed and developed. Based on the competitive Multi-Dot-ELISA, real samples from 5 of 15 (33%) commercial cosmetic products were found CPFX and SMD additives which are banned in China. We demonstrate that this rapid and low-cost platform is a promising tool in human health protection.

show abstract

“…However, complicated pre-treatment and high cost have limited their application in point-of-care testing (POCT). Immunoassay methods mainly include enzyme-linked immunosorbent assay (ELISA) [ 5 , 9 , 10 ] and gold lateral flow strip (GLFS). ELISA has been commonly used in detecting drug residues in virtue of its specificity, sensitivity, and low cost.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Lateral Flow Strip for the Detection of Cocaine in Urine by Naked Eyes and Smart Phone Camera

Dong

Zhang

et al. 2017

Sensors

View full text Add to dashboard Cite

Magnetic lateral flow strip (MLFS) based on magnetic bead (MB) and smart phone camera has been developed for quantitative detection of cocaine (CC) in urine samples. CC and CC-bovine serum albumin (CC-BSA) could competitively react with MB-antibody (MB-Ab) of CC on the surface of test line of MLFS. The color of MB-Ab conjugate on the test line relates to the concentration of target in the competition immunoassay format, which can be used as a visual signal. Furthermore, the color density of the MB-Ab conjugate can be transferred into digital signal (gray value) by a smart phone, which can be used as a quantitative signal. The linear detection range for CC is 5–500 ng/mL and the relative standard deviations are under 10%. The visual limit of detection was 5 ng/mL and the whole analysis time was within 10 min. The MLFS has been successfully employed for the detection of CC in urine samples without sample pre-treatment and the result is also agreed to that of enzyme-linked immunosorbent assay (ELISA). With the popularization of smart phone cameras, the MLFS has large potential in the detection of drug residues in virtue of its stability, speediness, and low-cost.

show abstract

A 96-well Plate Based Dot-ELISA Array for Simultaneous Detection of Multi-Drugs

Cited by 10 publications

References 18 publications

Use of Carbon Nanotubes as a Solid Support To Establish Quantitative (Centrifugation) and Qualitative (Filtration) Immunoassays To Detect Gentamicin Contamination in Commercial Milk

Use of Carbon Nanotubes as a Solid Support To Establish Quantitative (Centrifugation) and Qualitative (Filtration) Immunoassays To Detect Gentamicin Contamination in Commercial Milk

Development of a High-Throughput Immunoassay for Rapid Detection of Multiple Antibiotic Residues in Cosmetics

Magnetic Lateral Flow Strip for the Detection of Cocaine in Urine by Naked Eyes and Smart Phone Camera

Contact Info

Product

Resources

About