A B‐box transcription factor <scp>OsBBX17</scp> regulates saline‐alkaline tolerance through the <scp>MAPK</scp> cascade pathway in rice

Shen, Tao; Xu, Fengjuan; Chen, Dan; Yan, Runjiao; Wang, Qingwen; Li, Kaiyue; Zhang, Gang; Ni, Lan; Jiang, Mingyi

doi:10.1111/nph.19480

Cited by 4 publications

(1 citation statement)

References 88 publications

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“…5 μg kinase OsDMI3-GST was incubated with 5 μg substrates (OsPrx20-His, Os Prx20 T244A -His, or myelin basic protein [MBP; Sigma-Aldrich]) in kinase reaction solution for 30 min as described previously (Shen et al, 2023b) . 5×SDS-PAGE loading buffer was added to terminate the reaction, and the reaction mixtures were separated by 15% SDS-PAGE.…”

Section: Methodsmentioning

confidence: 99%

The Class III peroxidase OsPrx20 is a key regulator of stress response and growth in rice

Shen,

Wang,

Chen

et al. 2024

Preprint

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In plants, reactive oxygen species maintain strictly low intracellular levels that is prerequisite for their function as second messengers. However, abiotic and biotic stresses are also long-term processes, requiring rapid scavenging of excess intracellular ROS. Plant Class III peroxidases, as multifunctional enzymes, are core enzymes in regulating intracellular ROS homeostasis and are key to regulating the complicated signaling network. Here, we found a rice Class III peroxidase OsPrx20 maintains intracellular ROS homeostasis in different conditions. OsPrx20 is a target of Ca2+/calmodulin-dependent protein kinase (OsDMI3) and phosphorylates its Thr-244 site by OsDMI3. Overexpression of OsPrx20 enhances osmotic stress tolerance but reduces blast resistance, while lack of OsPrx20 is opposite, suggesting OsPrx20 positively regulates osmotic stress tolerance but negatively regulates blast resistance in rice. Meanwhile, overexpression of OsPrx20 enlarges spike size and grain fullness, whereas lack of OsPrx20 leads to dwarfism and smaller spike. In ABA signaling, OsPrx20 Thr-244 phosphorylation is specifically dependent on OsDMI3 to reduce the sensitivity of ABA to seed germination and root growth and to enhance osmotic stress tolerance without affecting spike and grain development. Our study reveals an essential regulatory mechanism that directly activates OsPrx20 in ABA signaling, highlighting the multi-functionality of OsPrx20 in different biological processes.

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Section: Methodsmentioning

confidence: 99%

The Class III peroxidase OsPrx20 is a key regulator of stress response and growth in rice

Shen,

Wang,

Chen

et al. 2024

Preprint

Self Cite

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MdLRR-RLK1-MdATG3 module enhances the resistance of apples to abiotic stress via autophagy

Chen,

Guo,

Zhang

et al. 2024

Preprint

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LRR-RLKs play a key role in plant responses to stress, although their physiological functions under abiotic stress are not yet fully understood. MdLRR-RLK1 was found to regulate plant growth and development and to increase tolerance to salt and drought stress. MdLRR-RLK1 interacts with MdATG3, which ubiquitinates and degrades MdLRR-RLK1. MdATG3 enhances salt and drought tolerance through autophagy, interacting with MdATG8F/I-like in apple. These findings reveal the interaction between MdLRR-RLK1 and MdATG3, suggesting mechanisms that regulate apple growth and resistance to abiotic stress.

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MAPKKK28 functions upstream of the MKK1‐MPK1 cascade to regulate abscisic acid responses in rice

Ren,

Zhang,

Yang

et al. 2024

Plant Cell & Environment

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The mitogen‐activated protein kinase (MAPK) cascade (MAPKKK‐MAPKK‐MAPK) plays a critical role in biotic and abiotic stress responses and abscisic acid (ABA) signalling. A previous study has shown that the ABA‐activated MKK1‐MPK1 cascade is essential in regulating ABA response and stress tolerance in rice. However, the specific MAPKKK upstream of the MKK1‐MPK1 cascade in ABA signalling remains unknown. Here, we identified that MAPKKK28, a previously uncharacterized member of the rice MEKK family, is involved in regulating ABA responses, including seed germination, root growth, stomatal closure, and the tolerance to oxidative stress and osmotic stress. We found that MAPKKK28 directly interacts with and phosphorylates MKK1. Further analysis indicated that the activation of both MKK1 and MPK1 depends on MAPKKK28 in ABA signalling. Genetic analysis revealed that MAPKKK28 functions upstream of the MKK1‐MPK1 cascade to positively regulate ABA responses and enhance tolerance to oxidative and osmotic stress. These results not only reveal a new complete MAPK cascade in plants but also uncover its importance in ABA signalling.

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A B‐box transcription factor OsBBX17 regulates saline‐alkaline tolerance through the MAPK cascade pathway in rice

Cited by 4 publications

References 88 publications

The Class III peroxidase OsPrx20 is a key regulator of stress response and growth in rice

The Class III peroxidase OsPrx20 is a key regulator of stress response and growth in rice

MdLRR-RLK1-MdATG3 module enhances the resistance of apples to abiotic stress via autophagy

MAPKKK28 functions upstream of the MKK1‐MPK1 cascade to regulate abscisic acid responses in rice

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