A B-box zinc finger protein, MdBBX10, enhanced salt and drought stresses tolerance in Arabidopsis

Liu, Xin; Li, Rong; Dai, Yaqing; Li, Yuan; Sun, Qinghua; Zhang, Shizhong; Wang, Xiaoyun

doi:10.1007/s11103-019-00828-8

Cited by 56 publications

(49 citation statements)

References 39 publications

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“…One grape B-box ZFP family, VvZFPL, has been reported to show increased tolerance to cold, drought and salt stresses in transgenic Arabidopsis plants. Additionally, as a B-box type zinc finger protein, MdBBX10 has been revealed to exhibit enhanced tolerance to salt and drought stresses in Arabidopsis [59][60][61]. Therefore, all the above findings support that GmSFT might be the candidate gene regulating seed-flooding tolerance in soybean.…”

Section: Discussionmentioning

confidence: 69%

Identification of QTN and Candidate Gene for Seed-flooding Tolerance in Soybean [Glycine max (L.) Merr.] using Genome-Wide Association Study (GWAS)

Chang

et al. 2019

Genes

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Seed-flooding stress is one of the major abiotic constraints severely affecting soybean yield and quality. Understanding the molecular mechanism and genetic basis underlying seed-flooding tolerance will be of greatly importance in soybean breeding. However, very limited information is available about the genetic basis of seed-flooding tolerance in soybean. The present study performed Genome-Wide Association Study (GWAS) to identify the quantitative trait nucleotides (QTNs) associated with three seed-flooding tolerance related traits, viz., germination rate (GR), normal seedling rate (NSR) and electric conductivity (EC), using a panel of 347 soybean lines and the genotypic data of 60,109 SNPs with MAF > 0.05. A total of 25 and 21 QTNs associated with all three traits were identified via mixed linear model (MLM) and multi-locus random-SNP-effect mixed linear model (mrMLM) in three different environments (JP14, HY15, and Combined). Among these QTNs, three major QTNs, viz., QTN13, qNSR-10 and qEC-7-2, were identified through both methods MLM and mrMLM. Interestingly, QTN13 located on Chr.13 has been consistently identified to be associated with all three studied traits in both methods and multiple environments. Within the 1.0 Mb physical interval surrounding the QTN13, nine candidate genes were screened for their involvement in seed-flooding tolerance based on gene annotation information and available literature. Based on the qRT-PCR and sequence analysis, only one gene designated as GmSFT (Glyma.13g248000) displayed significantly higher expression level in all tolerant genotypes compared to sensitive ones under flooding treatment, as well as revealed nonsynonymous mutation in tolerant genotypes, leading to amino acid change in the protein. Additionally, subcellular localization showed that GmSFT was localized in the nucleus and cell membrane. Hence, GmSFT was considered as the most likely candidate gene for seed-flooding tolerance in soybean. In conclusion, the findings of the present study not only increase our knowledge of the genetic control of seed-flooding tolerance in soybean, but will also be of great utility in marker-assisted selection and gene cloning to elucidate the mechanisms of seed-flooding tolerance.

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Section: Discussionmentioning

confidence: 69%

Identification of QTN and Candidate Gene for Seed-flooding Tolerance in Soybean [Glycine max (L.) Merr.] using Genome-Wide Association Study (GWAS)

Chang

et al. 2019

Genes

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“…The BBX proteins play critical roles in the control of hypocotyl elongation, pigment accumulation, flowering and stress responses in crop plants, including rosaceous fruit tree crops (Bai et al ., , ; Liu et al ., ). The present study shows that two BBX proteins, PpBBX18 and PpBBX21, antagonistically regulate anthocyanin accumulation in pear.…”

Section: Discussionmentioning

confidence: 97%

Two B‐box proteins, PpBBX18 and PpBBX21, antagonistically regulate anthocyanin biosynthesis via competitive association with Pyrus pyrifolia ELONGATED HYPOCOTYL 5 in the peel of pear fruit

et al. 2019

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Light is indispensable for the accumulation of anthocyanin in the peel of red pear fruit (Pyrus pyrifolia Nakai). ELONGATED HYPOCOTYL 5 (HY5) is considered to be a critical regulator for induction of anthocyanin biosynthesis, but detailed characterization of its regulatory mechanism is needed. In this study, multiple genetic and biochemical approaches were applied to identify the roles of P. pyrifolia HY5 (PpHY5) and two B-box (BBX) proteins, PpBBX18 and PpBBX21, in the transcriptional regulation of PpMYB10. The functions of the two BBX proteins were analyzed in overexpression lines using pear calli-based approaches. On its own PpHY5 was unable to activate downstream genes. The two BBX proteins, PpBBX18 and PpBBX21, physically interacted with PpHY5 and antagonistically regulated anthocyanin biosynthesis in Arabidopsis and pear. PpBBX18 formed a heterodimer with PpHY5 via two B-box domains, in which PpHY5 bound to the G-box motif of PpMYB10 and PpBBX18 provided the trans-acting activity, thus inducing transcription of PpMYB10. PpBBX21 interacted with PpHY5 and PpBBX18 and hampered formation of the PpHY5-PpBBX18 active transcription activator complex, and subsequently repressed anthocyanin biosynthesis. The present results demonstrate the fine-tuned regulation of anthocyanin biosynthesis via transcriptional regulation of PpMYB10 by PpHY5-associated proteins and provide insights into light-induced anthocyanin biosynthesis.

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“…In dicotyledonous plant species, two BBX genes from Arabidopsis, BBX5 and BBX24 (also called AtCOL4 and STO), have been linked to osmotic stress tolerance, also through the ABA-dependant signalling pathway (Min et al 2015;Nagaoka and Takano 2003). The overexpression of MdBBX10 from apple enhanced drought stress tolerance in transgenic Arabidopsis by enhancing the plants' ability to scavenge reactive oxygen species (Liu et al 2019). Similarly, in the promoter regions of the tomato genes SIBBX7 and SlBBX12, ABA responsive elements were identified, which most likely also indicated a link to drought responses (Chu et al 2016).…”

Section: Discussionmentioning

confidence: 99%

“…The majority of these genes also responded to an ABA input signal leading to increased expression. Specifically, in relation to drought tolerance, the MdBBX10 gene from apple was upregulated under salt and osmotic stresses and when overexpressed in Arabidopsis enhanced drought tolerance by increasing the transgenic plants' ability to scavenge reactive oxygen species (Liu et al 2019). The rice zinc finger protein, OsMSR15 containing a B-box motif, enhanced drought tolerance in Arabidopsis.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of AtBBX29 Improves Drought Tolerance by Maintaining Photosynthesis and Enhancing the Antioxidant and Osmolyte Capacity of Sugarcane Plants

2020

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B-box proteins have emerged as prominent mechanisms for controlling growth and developmental processes and in some instances responses to biotic and abiotic stresses in plants. These proteins mediate transcriptional regulations and protein–protein interactions in cellular signalling processes. B-box proteins thereby play an important role in coordinating physiological and biochemical pathway flux and are therefore ideal targets for controlling stress responses in plants. In this study, the overexpression of an Arabidopsis thaliana B-box gene (BBX29) in sugarcane (Saccharum spp. hybrid) has led to enhanced drought tolerance and delayed senescence under water-deficit conditions when compared to the wild-type plants. Transgenic plants maintained a higher relative water content and better protected its photosynthetic machinery. These plants accumulated more proline and displayed enhanced enzymatic antioxidant activity under drought conditions. Overexpression of AtBBX29 further alleviated the build-up of reactive oxygen species and curtailed oxidative damage, resulting in transgenic plants with improved health and higher survival rates during dehydration. Our results suggested that the AtBBX29 gene influenced an array of physiological and biochemical mechanisms in sugarcane to the advantage of the crop and might be a target to genetically engineer drought tolerance into sugarcane. This is the first report to elucidate B-box protein functionality in a polyploid crop such as sugarcane.

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A B-box zinc finger protein, MdBBX10, enhanced salt and drought stresses tolerance in Arabidopsis

Cited by 56 publications

References 39 publications

Identification of QTN and Candidate Gene for Seed-flooding Tolerance in Soybean [Glycine max (L.) Merr.] using Genome-Wide Association Study (GWAS)

Identification of QTN and Candidate Gene for Seed-flooding Tolerance in Soybean [Glycine max (L.) Merr.] using Genome-Wide Association Study (GWAS)

Two B‐box proteins, PpBBX18 and PpBBX21, antagonistically regulate anthocyanin biosynthesis via competitive association with Pyrus pyrifolia ELONGATED HYPOCOTYL 5 in the peel of pear fruit

Overexpression of AtBBX29 Improves Drought Tolerance by Maintaining Photosynthesis and Enhancing the Antioxidant and Osmolyte Capacity of Sugarcane Plants

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