A Bacillus subtilis (QST 713) spore-based probiotic for necrotic enteritis control in broiler chickens

Tactacan, G. B.; Schmidt, J.; Miille, M. J.; Jiménez, Duberney Rangel

doi:10.3382/japr.2013-00730

Cited by 40 publications

(28 citation statements)

References 15 publications

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“…In a C. perfringens/Eimeria maxima coinfection model of avian necrotic enteritis, feeding of B. subtilis reduced gut pathology and animal mortality, and restored alterations in the intestinal microbiome, compared with controls (22). Other studies have confirmed the ability of Bacillus DFMs to reduce C. perfringens colonization of the intestinal mucosa and improve animal growth performance in avian necrotic enteritis (36)(37)(38)(39)(40)(41). In general, while the beneficial effect of Bacillus DFMs on body weight gains in healthy, uninfected chickens is generally moderate (<10%), a more pronounced outcome of the dietary supplements has been observed when weight loss is aggravated as a consequence of pathogenic infection (42).…”

Section: Discussionmentioning

confidence: 92%

Dietary Supplementation With Bacillus subtilis Direct-Fed Microbials Alters Chicken Intestinal Metabolite Levels

Park

Zimmerman²,

Smith³

et al. 2020

Front. Vet. Sci.

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Direct-fed microbials (DFMs) are dietary supplements containing live microorganisms which confer a performance and health benefit to the host, but the mechanisms are unclear. Here, a metabolomics approach was used to identify changes in intestinal metabolite levels in chickens fed an unsupplemented diet or a diet supplemented with B. subtilis strain 1781 or strain 747. Body weight gains of chickens fed the B. subtilis-supplemented diets were increased up to 5.6% in the B. subtilis 1781 group and 7.6% in the B. subtilis 747 group compared with chickens fed the unsupplemented diet. Compared with unsupplemented controls, the levels of 83 metabolites were altered (p < 0.05) (25 increased, 58 decreased) in chickens given the B. subtilis 1781-supplemented diet, while 50 were altered (p < 0.05) (12 increased, 38 decreased) with the B. subtilis 747-supplemented diet. Twenty-two metabolites were altered (p < 0.05) (18 increased, 4 decreased) in the B. subtilis 1781 vs. B. subtilis 747 groups. A random forest analysis of the B. subtilis 1781 vs. control groups gave a predictive accuracy of 87.5%, while that of the B. subtilis 747 vs. control groups was 62.5%. A random forest analysis of the B. subtilis 1781 vs. B. subtilis 747 groups gave a predictive accuracy of 75.0%. Changes in the levels of these intestinal biochemicals provided a distinctive biochemical signature unique to each B. subtilis-supplemented group, and were characterized by alterations in the levels of dipeptides (alanylleucine, glutaminylleucine, phenylalanylalanine, valylglutamine), nucleosides (N1-methyladenosine, N6-methyladenosine, guanine, 2-deoxyguanosine), fatty acids (sebacate, valerylglycine, linoleoylcholine), and carbohydrates (fructose). These results provide the foundation for future studies to identify biochemicals that might be used to improve poultry growth performance in the absence of antibiotic growth promoters.

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Section: Discussionmentioning

confidence: 92%

Dietary Supplementation With Bacillus subtilis Direct-Fed Microbials Alters Chicken Intestinal Metabolite Levels

Park

Zimmerman²,

Smith³

et al. 2020

Front. Vet. Sci.

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“…Many studies have reported that dietary supplementation of Bacillus subtilis can prevent NE in broiler chickens by competitive exclusion of Clostridium perfringens in the gastrointestinal tract (La Ragione and Woodward, 2003;Tactacan et al, 2013;Jayaraman et al, 2013;Cheng et al, 2018). It has been shown that Bacillus licheniformis isolated from broiler gastrointestinal tract reveals antimicrobial activity against a broad spectrum of pathogens, such as Clostridium perfringens in vitro (Barbosa et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Optimization of solid-state fermentation conditions of Bacillus licheniformis and its effects on Clostridium perfringens-induced necrotic enteritis in broilers

et al. 2019

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Non-ruminants Full-length research article Optimization of solid-state fermentation conditions of Bacillus licheniformis and its effects on Clostridium perfringens-induced necrotic enteritis in broilers ABSTRACT-In the present study, we examined the growth parameters of Bacillus licheniformis in solid-state fermentation (SSF) and evaluated the effects of Bacillus licheniformis-fermented products on Clostridium perfringens-challenged broilers. During four and six days of SSF, the highest viable biomass was observed at 5% glucose, 10% soybean meal, 3% yeast, and 50% initial moisture content. The Bacillus licheniformis SSF products were heat-and acid-resistant. Furthermore, the fermented products were able to inhibit the growth of Clostridium perfringens and Staphylococcus aureus in vitro. In feeding experiments, in a similar manner to the antibiotic treatment group, dietary supplementation of Bacillus licheniformis-fermented products significantly improved intestinal morphology and necrotic lesions under Clostridium perfringens challenge, accompanied by increased IFN-γ mRNA expression in the spleen and bursa of Fabricius. These results together suggest that Bacillus licheniformis-fermented products have potential for development as feed additives and use as possible substitutes for antibiotics to treat Clostridium perfringens in the poultry industry.

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“…H&E x 400. This result explained by (Knap et al, 2010 andTactacan et al, 2013),they reported that a strain of Bacillus licheniformis, inhibited the subsequent establishment of a Clostridium perfringens strain ingested by the animals. This inhibitory effect depended on the in vivo production by B. licheniformis of an antibiotic substance having a number of the characteristics of bacitracin which is effective on Clostridium.…”

Section: Discussionmentioning

confidence: 90%

Role of Saltose Probiotic for the Control of the Experimental Infection of the Clostridium Perfringens and the Coccidia in Chickens

Belih¹,

Labib²,

Ragab³

2015

AJVS

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Two hundred and forty, one day old broiler chicken were randomly divided into eight equal groups. The 1 st group was control. The 2 nd group was given saltose from the 1 st day of age till the end of experiment at a dose 1gm / kg of ration. The 3 rd group was infected intra crop with inoculums containing 0.5x10 9 cfu ̸ ml Clostridium perfringens at 15 days of age .The 4 th group infected intra crop with 75000 sporulated oocyte of Eimeria necatrix at 15 days of age .The 5 th group was infected with inoculums containing 0.5x10 9 cfu ̸ ml Clostridium perfringens and 75000 sporulated oocyts of Eimeria necatrix intra crop at 15 days of age. The 6 th group given saltose from the 1 st day and infected with Clostridium perfringens as in group3. The 7 th group was given saltose as in group 2 and infected with Eimeria necatrix as in the 4 th group. The 8 th group was given saltose as in group 2 and infected with Clostridium perfringens and Eimeria necatrix as in group 5. Three blood samples were collected from all groups at 21 days and at 28 days of age. The 1 st blood sample was collected with anticoagulant for RBCѕ and differential leucocytic count. The 2 nd blood sample was collected without anticoagulant for serum separation for measuring ALT, AST and alkaline phosphatase and IgA. The 3 rd blood sample was collected by heart puncture under strict aseptic condition on heparin for immunological examination. Necropsy was performed and the intestinal tracts of sacrificed chickens of all challenged groups (groups 3, 5, 6, 8) at 7, 14 and 21 days post challenged were collected for bacterial count and for DNA analysis by PCR to recognize the enterotoxin gene that produced during sporulation and demonstrate the efficacy of Saltose on the cell wall microorganism . Also tissue specimens were collected from the intestine (small, large and ceci), liver, kidneys, heart and spleen, fixed in 10% buffered neutral formalin for Pathological examination.The results showed that the probiotic Saltose reduced the severity of the necrotic enteritis due to Clostridium perfringens infection through the intestinal bacterial count, DNA analysis by PCR, hematological, biochemical and histopathological findings, also alleviated the pathological changes demonstrated by Eimeria necatrix infestation.These results imply that the Saltose based probiotic was able to reduce the severities of necrotic enteritis (NE) and ameliorate the pathological changes associated with coccidiosis.

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A Bacillus subtilis (QST 713) spore-based probiotic for necrotic enteritis control in broiler chickens

Cited by 40 publications

References 15 publications

Dietary Supplementation With Bacillus subtilis Direct-Fed Microbials Alters Chicken Intestinal Metabolite Levels

Dietary Supplementation With Bacillus subtilis Direct-Fed Microbials Alters Chicken Intestinal Metabolite Levels

Optimization of solid-state fermentation conditions of Bacillus licheniformis and its effects on Clostridium perfringens-induced necrotic enteritis in broilers

Role of Saltose Probiotic for the Control of the Experimental Infection of the Clostridium Perfringens and the Coccidia in Chickens

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