2017
DOI: 10.1038/srep44422
|View full text |Cite
|
Sign up to set email alerts
|

A bacterial aromatic aldehyde dehydrogenase critical for the efficient catabolism of syringaldehyde

Abstract: Vanillin and syringaldehyde obtained from lignin are essential intermediates for the production of basic chemicals using microbial cell factories. However, in contrast to vanillin, the microbial conversion of syringaldehyde is poorly understood. Here, we identified an aromatic aldehyde dehydrogenase (ALDH) gene responsible for syringaldehyde catabolism from 20 putative ALDH genes of Sphingobium sp. strain SYK-6. All these genes were expressed in Escherichia coli, and nine gene products, including previously ch… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

1
36
0

Year Published

2017
2017
2024
2024

Publication Types

Select...
6
1

Relationship

0
7

Authors

Journals

citations
Cited by 55 publications
(37 citation statements)
references
References 48 publications
1
36
0
Order By: Relevance
“…Syringic acid can be produced from syringaldehyde, one of the lignin‐derived aromatic molecules, by heterologous expression of desV or ligV in E. coli . In further, syringic acid could be converted by the demethylase (desA and ligM ) to gallic acid, which is one of naturally occurring phytochemicals, and has strong antioxidant and antibacterial activities .…”
Section: Synthetic Biology Applications In Lignin Valorizationmentioning
confidence: 99%
See 1 more Smart Citation
“…Syringic acid can be produced from syringaldehyde, one of the lignin‐derived aromatic molecules, by heterologous expression of desV or ligV in E. coli . In further, syringic acid could be converted by the demethylase (desA and ligM ) to gallic acid, which is one of naturally occurring phytochemicals, and has strong antioxidant and antibacterial activities .…”
Section: Synthetic Biology Applications In Lignin Valorizationmentioning
confidence: 99%
“…Besides PCA decarboxylation, catechol can also be produced from guaiacol, a product of lignin depolymerization, via expressing a cytochrome P450 aromatic O-demethylase from the family CYP255A (GcoA) and a three-domain reductase (GcoB) in P. putida [46]. Syringic acid can be produced from syringaldehyde, one of the lignin-derived aromatic molecules, by heterologous expression of desV or ligV in E. coli [47]. In further, syringic acid could be converted by the demethylase (desA and ligM) to gallic acid, which is one of naturally occurring phytochemicals, and has strong antioxidant and antibacterial activities [15].…”
Section: Production Of Aromatic Chemicalsmentioning
confidence: 99%
“…Decomposition of all three phenylpropanoids proceed via conjugation of the γ-acid to Coenzyme A (CoA), and cleavage of one unit of acetyl-CoA [108,109]. Two cleavage pathways are known, one resulting an aromatic aldehyde (p-hydroxybenzaldehyde/vanillin/syringaldehyde) [110] and another resulting an aromatic acid (benzoate/vanillate/syringate) [111]. Aromatic aldehydes produced in these pathways are oxidized to aromatic acids by one of several aromatic aldehyde dehydrogenases [112].…”
Section: Phenylpropanoidsmentioning
confidence: 99%
“…The latter pathway is present in Sphingobium sp. SYK-6, with FerA and FerB performing the deacetylation 16 , followed by oxidation by LigV or DesV 17,18 . Likewise, β-oxidative routes have been described for ferulate and coumarate in Rhodococcus jostii RHA1 19 and for ferulate, coumarate, and caffeate in Agrobacterium fabrum 20 .…”
Section: Introductionmentioning
confidence: 99%
“…SARO_RS14535 and SARO_RS14545 have homology to the phenylhydroxypropionyl-CoA dehydrogenase Atu1415 and the 4-hydroxyphenyl-beta-ketoacyl-CoA hydrolase Atu1421 from A. fabrum , respectively 20 . By homology, SARO_RS14550 is likely to be an aromatic aldehyde dehydrogenase, presumably with similar functionality as LigV or DesV 17 . We hypothesize that these enzymes act in parallel with FerA, FerB, and LigV to deacetylate cinnamic acid derivatives.…”
Section: Introductionmentioning
confidence: 99%