A bacterial artificial chromosome (<scp>BAC</scp>) genomic approach reveals partial clustering of the furanocoumarin pathway genes in parsnip

Roselli, Sandro; Olry, Alexandre; Vautrin, Sonia; Coriton, Olivier; Ritchie, David W.; Galati, Gianni; Navrot, Nicolas; Krieger, Célia; Vialart, Guilhem; Bergès, Hélène; Bourgaud, Frédéric; Hehn, Alain

doi:10.1111/tpj.13450

Cited by 26 publications

(26 citation statements)

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“…In plants, this phenomenon has been increasingly documented for chemical defense pathways (Takos & Rook, 2012;Boycheva et al, 2014). First described for a benzoxazinoid gene cluster in maize (Frey et al, 1997), metabolic clusters have subsequently been detected in diverse angiosperm species for di-and triterpenes (Qi et al, 2004;Field & Osbourn, 2008;Matsuba et al, 2013), steroidal and isoquinoline alkaloids (Winzer et al, 2012;Itkin et al, 2013), polyketides (Schneider et al, 2016), furanocoumarins (Roselli et al, 2017) and cyanogenic glucosides (CNglcs) (Takos et al, 2011;Boycheva et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Micro‐ and macroevolutionary adaptation through repeated loss of a complete metabolic pathway

Olsen

Small

2018

New Phytologist

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There is growing evidence for the convergent evolution of physically linked gene clusters encoding chemical defense pathways. Metabolic clusters are proposed to evolve because they ensure co-inheritance of all required genes where the defense is favored, and prevent inheritance of toxic partial pathways where it is not. This hypothesis rests on the assumption that clusters evolve in species where selection favors intraspecific polymorphism for the defense; however, they have not been examined in polymorphic species. We examined metabolic cluster evolution in relation to an adaptive polymorphism for cyanogenic glucoside (CNglc) production in clover. Using 163 accessions, we performed CNglc assays, BAC sequencing, Southern hybridizations and molecular evolutionary analyses. We find that the CNglc pathway forms a 138-kb cluster in white clover, and that the adaptive polymorphism occurs through presence/absence of the complete cluster. Component genes are orthologous to those in the distantly related legume Lotus japonicus. These findings provide empirical support for the co-inheritance hypothesis, and they indicate that adaptive CNglc variation in white clover evolves through recurrent deletions of the entire pathway. They further indicate that the shared ancestor of many important legume crops was likely cyanogenic and that this defense was lost repeatedly over the last 50 Myr.

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Section: Introductionmentioning

confidence: 99%

Micro‐ and macroevolutionary adaptation through repeated loss of a complete metabolic pathway

Olsen

Small

2018

New Phytologist

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“…Both genes also sit in a cluster on a separate chromosome from the cluster where UDT C6 and p-coumaryl CoA 2'hydroxylase genes, were located. Genes transcribing the enzymes involved upstream of the furocoumarin biosynthesis pathway were seen to cluster together with both UDT C6 and p-coumaryl CoA genes, while the other P450 genes were clustered together with CYP71A3 and CYP71A4 genes (Roselli et al, 2017). This study goes against the common idea that genes from a similar selfdefence pathway would be found together as an evolutionary advantage.…”

Section: The Biological Potential Of Angelicinmentioning

confidence: 69%

“…The synthesis of DMS would lead to the formation of psoralen while osthenol is the precursor of angelicin. DMS is then catalyzed by both marmesin synthase and psoralen synthase to form psoralen itself (Hamerski and Matern, 1988;Larbat et al, 2008;Roselli et al, 2017). On the other hand, for angular furocoumarins, osthenol is catalyzed by columbianetin synthase to form (+)-columbianetin (Roselli et al, 2017).…”

Section: The Biological Potential Of Angelicinmentioning

confidence: 99%

“…DMS is then catalyzed by both marmesin synthase and psoralen synthase to form psoralen itself (Hamerski and Matern, 1988;Larbat et al, 2008;Roselli et al, 2017). On the other hand, for angular furocoumarins, osthenol is catalyzed by columbianetin synthase to form (+)-columbianetin (Roselli et al, 2017). Angelicin synthase then catalyzes the conversion of (+)-columbianetin to angelicin through the abstraction of syn-C3'-hydrogen (Larbat et al, 2008).…”

Section: The Biological Potential Of Angelicinmentioning

confidence: 99%

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Angelicin—A Furocoumarin Compound With Vast Biological Potential

et al. 2020

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Angelicin, a member of the furocoumarin group, is related to psoralen which is well known for its effectiveness in phototherapy. The furocoumarins as a group have been studied since the 1950s but only recently has angelicin begun to come into its own as the subject of several biological studies. Angelicin has demonstrated anti-cancer properties against multiple cell lines, exerting effects via both the intrinsic and extrinsic apoptotic pathways, and also demonstrated an ability to inhibit tubulin polymerization to a higher degree than psoralen. Besides that, angelicin too demonstrated anti-inflammatory activity in inflammatory-related respiratory and neurodegenerative ailments via the activation of NF-kB pathway. Angelicin also showed pro-osteogenesis and pro-chondrogenic effects on osteoblasts and pre-chondrocytes respectively. The elevated expression of proosteogenic and chondrogenic markers and activation of TGF-b/BMP, Wnt/b-catenin pathway confirms the positive effect of angelicin bone remodeling. Angelicin also increased the expression of estrogen receptor alpha (ERa) in osteogenesis. Other bioactivities, such as anti-viral and erythroid differentiating properties of angelicin, were also reported by several researchers with the latter even displaying an even greater aptitude as compared to the commonly prescribed drug, hydroxyurea, which is currently on the market. Apart from that, recently, a new application for angelicin against periodontitis had been studied, where reduction of bone loss was indirectly caused by its anti-microbial properties. All in all, angelicin appears to be a promising compound for further studies especially on its mechanism and application in therapies for a multitude of

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“…A highly complete and accurate sequence of the region of interest is a prerequisite for successful gene cloning. The availability of BAC clones with known genomic context enables focused and affordable resequencing of the critical region with more advanced technologies, such as PacBio and Oxford Nanopore, facilitating the identification of the desired gene or gene cluster [24,25].…”

Section: Introductionmentioning

confidence: 99%

Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative studies

et al. 2019

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Bread wheat (Triticum aestivum L.) is a staple food for a significant part of the world's population. The growing demand on its production can be satisfied by improving yield and resistance to biotic and abiotic stress. Knowledge of the genome sequence would aid in discovering genes and QTLs underlying these traits and provide a basis for genomics-assisted breeding. Physical maps and BAC clones associated with them have been valuable resources from which to generate a reference genome of bread wheat and to assist map-based gene cloning. As a part of a joint effort coordinated by the International Wheat Genome Sequencing Consortium, we have constructed a BAC-based physical map of bread wheat chromosome arm 7DS consisting of 895 contigs and covering 94% of its estimated length. By anchoring BAC contigs to one radiation hybrid map and three high resolution genetic maps, we assigned 73% of the assembly to a distinct genomic position. This map integration, interconnecting a total of 1713 markers with ordered and sequenced BAC clones from a minimal tiling path, provides a tool to speed up gene cloning in wheat. The process of physical map assembly included the integration of the 7DS physical map with a whole-genome physical map of Aegilops tauschii and a 7DS Bionano genome map, which together enabled efficient scaffolding of physical-map contigs, even in the non-recombining region of the genetic centromere. Moreover, this approach facilitated a comparison of bread wheat and its ancestor at BAC-contig level and revealed a reconstructed region in the 7DS pericentromere.

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A bacterial artificial chromosome (BAC) genomic approach reveals partial clustering of the furanocoumarin pathway genes in parsnip

Cited by 26 publications

References 50 publications

Micro‐ and macroevolutionary adaptation through repeated loss of a complete metabolic pathway

Micro‐ and macroevolutionary adaptation through repeated loss of a complete metabolic pathway

Angelicin—A Furocoumarin Compound With Vast Biological Potential

Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative studies

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