A bacterial glycolipid essential for membrane protein integration

Fujikawa, Kohki; Mori, Shoko; Nishiyama, Kenichi; Shimamoto, Keiko

doi:10.1016/bs.accb.2022.09.005

Cited by 7 publications

(1 citation statement)

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“…Membrane protein integrase (MPIase) is a glycolipid found in the inner membrane of E. coli ( Figure 4C ) ( Fujikawa et al, 2022 ; Nishikawa et al, 2022 ). Although its name is unlikely to indicate a glycolipid, it is derived from the unique activity involved in membrane protein integration ( Nishiyama et al, 2006 ; Nishiyama et al, 2010 ; Nishiyama et al, 2012 ).…”

Section: Enterobacterial Common Antigen (Eca) and Membrane Protein In...mentioning

confidence: 99%

Structures, functions, and syntheses of glycero-glycophospholipids

Osawa,

Fujikawa,

Shimamoto

2024

Front. Chem.

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Biological membranes consist of integral and peripheral protein-associated lipid bilayers. Although constituent lipids vary among cells, membrane lipids are mainly classified as phospholipids, glycolipids, and sterols. Phospholipids are further divided into glycerophospholipids and sphingophospholipids, whereas glycolipids are further classified as glyceroglycolipids and sphingoglycolipids. Both glycerophospholipids and glyceroglycolipids contain diacylglycerol as the common backbone, but their head groups differ. Most glycerolipids have polar head groups containing phosphate esters or sugar moieties. However, trace components termed glycero-glycophospholipids, each possessing both a phosphate ester and a sugar moiety, exist in membranes. Recently, the unique biological activities of glycero-glycophospholipids have attracted considerable attention. In this review, we describe the structure, distribution, function, biosynthesis, and chemical synthetic approaches of representative glycero-glycophospholipids—phosphatidylglucoside (PtdGlc) and enterobacterial common antigen (ECA). In addition, we introduce our recent studies on the rare glycero-glyco“pyrophospho”lipid, membrane protein integrase (MPIase), which is involved in protein translocation across biomembranes.

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Section: Enterobacterial Common Antigen (Eca) and Membrane Protein In...mentioning

confidence: 99%

Structures, functions, and syntheses of glycero-glycophospholipids

Osawa,

Fujikawa,

Shimamoto

2024

Front. Chem.

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Structural Requirements of a Glycolipid MPIase for Membrane Protein Integration

Fujikawa

Han

Osawa

et al. 2023

Chemistry A European J

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MPIase is a glycolipid involved in membrane protein integration in the inner membrane of Escherichia coli. To overcome the trace amounts and heterogeneity of natural MPIase, we systematically synthesized MPIase analogs. Structure‐activity relationship studies revealed the contribution of distinctive functional groups and the effect of the MPIase glycan length on membrane protein integration activity. In addition, both the synergistic effects of these analogs with the membrane chaperone/insertase YidC, and the chaperone‐like activity of the phosphorylated glycan were observed. These results verified the translocon‐independent membrane integration mechanism in the inner membrane of E. coli, in which MPIase captures the highly hydrophobic nascent proteins via its characteristic functional groups, prevents protein aggregation, attracts the proteins to the membrane surface, and delivers them to YidC in order to regenerate its own integration activity.

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