A bacterial tubulovesicular network

Acehan, Devrim; Santarella-Mellwig, Rachel; Devos, Damien P.

doi:10.1242/jcs.137596

Cited by 42 publications

(45 citation statements)

References 20 publications

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“…1), as previously reported for 2D imaging (10,11,13). Because we were limited to 2D approaches, we could not interpret the topology of cell features such as nucleoids (that appear as single or multiple nucleoids in 2D images) or the degree of connectivity of cellular regions and membranes (16,17).…”

Section: Tem Confirms the Presence Of A Complex Endomembrane System Inmentioning

confidence: 95%

“…Because the cellular architecture of G. obscuriglobus is variable and dynamic (10,14,16,17), we performed TEM to compare the ultrastructure of cells from our cultures with that reported previously. We observed the characteristic endomembrane system (single-and double-layered membranes), condensed nucleoid(s), and many regions distal to Significance Eukaryotic (plant and animal) cells possess a nuclear membrane that separates the two stages of gene expression (transcription and translation), whereas prokaryotic (bacteria and archaea) cells lack the nuclear membrane barrier to colocated transcription and translation.…”

Section: Tem Confirms the Presence Of A Complex Endomembrane System Inmentioning

confidence: 99%

“…More recent electron tomography studies (14,(16)(17)(18) support (18) or refute (14,16,17) this interpretation. The studies of Acehan et al (16) and Santarella-Mellwig et al (17) suggest that the endomembranes constitute a highly invaginated cytoplasmic membrane (and thus an extension of the typical Gram-negative cell plan) and that cytoplasmic volumes are all interconnected. These conflicting interpretations support conflicting opinions about the evolutionary relationship of G. obscuriglobus to eukaryotes (20)(21)(22).…”

mentioning

confidence: 98%

“…The planctomycete bacterial species Gemmata obscuriglobus possesses an extensive endomembrane network (9)(10)(11)(12)(13)(14)(15)(16)(17)(18). Endocytosis-like behavior (15,19) suggests that cellular transport may be a primary function of the endomembrane network.…”

mentioning

confidence: 99%

“…On the basis of transmission electron microscopy (TEM), these membranes originally were proposed to be unique to the organism and distinct from the cytoplasmic membrane, forming compartments that enclose the cell's nucleoid(s) (10)(11)(12)(13). More recent electron tomography studies (14,(16)(17)(18) support (18) or refute (14,16,17) this interpretation. The studies of Acehan et al (16) and Santarella-Mellwig et al (17) suggest that the endomembranes constitute a highly invaginated cytoplasmic membrane (and thus an extension of the typical Gram-negative cell plan) and that cytoplasmic volumes are all interconnected.…”

mentioning

confidence: 99%

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Spatially segregated transcription and translation in cells of the endomembrane-containing bacterium Gemmata obscuriglobus

Gottshall

Seebart

Gatlin

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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The dogma of coupled transcription and translation in bacteria has been challenged by recent reports of spatial segregation of these processes within the relatively simple cellular organization of the model organisms Escherichia coli and Bacillus subtilis. The bacterial species Gemmata obscuriglobus possesses an extensive endomembrane system. The membranes generate a very convoluted intracellular architecture in which some of the cell's ribosomes appear to have less direct access to the cell's nucleoid(s) than others. This observation prompted us to test the hypothesis that a substantial proportion of G. obscuriglobus translation may be spatially segregated from transcription. Using immunofluorescence and immunoelectron microscopy, we showed that translating ribosomes are localized throughout the cell, with a quantitatively greater proportion found in regions distal to nucleoid(s). Our results extend information about the phylogenetic and morphological diversity of bacteria in which the spatial organization of transcription and translation has been studied. These findings also suggest that endomembranes may provide an obstacle to colocated transcription and translation, a role for endomembranes that has not been reported previously for a prokaryotic organism. Our studies of G. obscuriglobus may provide a useful background for consideration of the evolutionary development of eukaryotic cellular complexity and how it led to decoupled processes of gene expression in eukaryotes.

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Section: Tem Confirms the Presence Of A Complex Endomembrane System Inmentioning

confidence: 95%

Section: Tem Confirms the Presence Of A Complex Endomembrane System Inmentioning

confidence: 99%

mentioning

confidence: 98%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Spatially segregated transcription and translation in cells of the endomembrane-containing bacterium Gemmata obscuriglobus

Gottshall

Seebart

Gatlin

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Gemmata

Fuerst

Butler

2022

Bergey's Manual of Systematics of Archaea and Bacteria

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Gem.ma'ta. L. past. part. used as L. fem. n. (from L. v. gemmare , to put forth buds), Gemmata , one provided with buds. Planctomycetota / Planctomycetia / Gemmatales / Gemmataceae / Gemmata Gem.ma'ta. L. fem. adj. used as L. fem. n. Gemmata, one provided with buds. The genus Gemmata belongs to the family Gemmataceae of the order Gemmatales (phylum Planctomycetota/ class Planctomycetia ) and comprises two named species, the type species Gemmata obscuriglobus , from a freshwater dam, and “ Gemmata massiliana ,” from a hospital water distribution system. Gemmata species are aerobic chemoorganotrophic heterotrophs for which carbohydrates are the carbon and energy sources, but the type species has the ability to take up proteins or polysaccharides from the external milieu. The type species and other members of the genus possess spherical cells, reproduce via budding, are motile via multitrichous flagella, and grow as pink colonies. The surfaces of whole cells, when observed by electron microscopy, have uniformly distributed round pit‐like crateriform structures, and at least in the type strain, sectioned cells viewed by electron microscopy display complex intracellular membranes. DNA G + C content (mol%) : 64–67 (genome sequence). Type species : Gemmata obscuriglobus Franzmann and Skerman 1984, VL18 emend. Scheuner et al. 2014.

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Navigating the Nuclear Envelope: One or Multiple Transport Mechanisms for Integral Membrane Proteins?

Dixon

Schirmer

2018

Nucleic Acids and Molecular Biology

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A bacterial tubulovesicular network

Cited by 42 publications

References 20 publications

Spatially segregated transcription and translation in cells of the endomembrane-containing bacterium Gemmata obscuriglobus

Spatially segregated transcription and translation in cells of the endomembrane-containing bacterium Gemmata obscuriglobus

Gemmata

Navigating the Nuclear Envelope: One or Multiple Transport Mechanisms for Integral Membrane Proteins?

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