A bacteriophage endolysin-based electrochemical impedance biosensor for the rapid detection of Listeria cells

Tolba, Mona; Ahmed, Minhaz Uddin; Tlili, Chaker; Eichenseher, Fritz; Loessner, Martin J.; Zourob, Mohammed

doi:10.1039/c2an35988j

Cited by 125 publications

(66 citation statements)

References 38 publications

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“…Certain bioreceptors have been developed to target a specific one of these moieties; for example, lectins, a type of carbohydrate binding protein, can be employed as bioreceptors for specific cell envelope sugars (28,29). Bacteriophages, viruses which bind to specific bacterial receptor proteins in order to infect the host cells, have also been employed for bacterial detection (30,31). Polyclonal antibodies raised against specific bacterial strains are the most commonly used bioreceptors for whole bacterial cell detection, where the binding targets on the cell envelope are usually unknown.…”

Section: Biosensors For Whole Bacterial Cell Detectionmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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SUMMARY Bacterial pathogens are important targets for detection and identification in medicine, food safety, public health, and security. Bacterial infection is a common cause of morbidity and mortality worldwide. In spite of the availability of antibiotics, these infections are often misdiagnosed or there is an unacceptable delay in diagnosis. Current methods of bacterial detection rely upon laboratory-based techniques such as cell culture, microscopic analysis, and biochemical assays. These procedures are time-consuming and costly and require specialist equipment and trained users. Portable stand-alone biosensors can facilitate rapid detection and diagnosis at the point of care. Biosensors will be particularly useful where a clear diagnosis informs treatment, in critical illness (e.g., meningitis) or to prevent further disease spread (e.g., in case of food-borne pathogens or sexually transmitted diseases). Detection of bacteria is also becoming increasingly important in antibioterrorism measures (e.g., anthrax detection). In this review, we discuss recent progress in the use of biosensors for the detection of whole bacterial cells for sensitive and earlier identification of bacteria without the need for sample processing. There is a particular focus on electrochemical biosensors, especially impedance-based systems, as these present key advantages in terms of ease of miniaturization, lack of reagents, sensitivity, and low cost.

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Section: Biosensors For Whole Bacterial Cell Detectionmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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“…Thus, R ct variation due to bacteriophages binding to the host bacteria is somewhat different from other bioreceptors in that at the beginning R ct increases due to bacterial binding hindering the redox reaction at the WE surface, while later it decreases due to cell lyses allowing the release of cell ionic material. Bacteriophages have been used as immobilizing bioreceptors in biosensors for different bacterial species (Shabani et al, 2008;Gervais et al, 2007;Tolba et al, 2012), as well as for an unconventional approach to IM where bacteriophages are integrated into the culture broth, and the monitored variation of electrolyte resistance is mainly due to conductivity increase induced by cell lyses (and not only due to bacterial metabolism) (Mortari et al, 2015). According to the authors, this approach results in much better performances with a detection limit of 1 CFU mL −1 or lower and response time less than 1 h.…”

Section: Impedance Biosensorsmentioning

confidence: 99%

Electrical impedance spectroscopy (EIS) for biological analysis and food characterization: a review

Grossi

Riccò

2017

J. Sens. Sens. Syst.

311

160

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Abstract. Electrical impedance spectroscopy (EIS), in which a sinusoidal test voltage or current is applied to the sample under test to measure its impedance over a suitable frequency range, is a powerful technique to investigate the electrical properties of a large variety of materials. In practice, the measured impedance spectra, usually fitted with an equivalent electrical model, represent an electrical fingerprint of the sample providing an insight into its properties and behavior. EIS is used in a broad range of applications as a quick and easily automated technique to characterize solid, liquid, semiliquid, organic as well as inorganic materials. This paper presents an updated review of EIS main implementations and applications.

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“…313 Electrodes modified with thiol self-assembled monolayers were also used for the detection of small organic molecules such as pesticides and toxins, 311,[333][334][335][336][337] drugs 309,338 and explosives. 339,340 Proteins 341-344 and bacteria 306,312,314,[345][346][347][348] can also be detected using electrodes modified with a thiol monolayer. The target analyte is accumulated at the surface of the chemically modified electrode at open-circuit potential.…”

Section: Accumulation Sensorsmentioning

confidence: 99%