A banana NAC transcription factor (MusaSNAC1) impart drought tolerance by modulating stomatal closure and H2O2 content

Abstract

MusaSNAC1 function in HO mediated stomatal closure and promote drought tolerance by directly binding to CGT[A/G] motif in regulatory region of multiple stress-related genes. Drought is a abiotic stress-condition, causing reduced plant growth and diminished crop yield. Guard cells of the stomata control photosynthesis and transpiration by regulating CO exchange and water loss, thus affecting growth and crop yield. Roles of NAC (NAM, ATAF1/2 and CUC2) protein in regulation of stress-conditions has been well documented however, their control over stomatal aperture is largely unknown. In this study we report a banana NAC protein, MusaSNAC1 which induced stomatal closure by elevating HO content in guard cells during drought stress. Overexpression of MusaSNAC1 in banana resulted in higher number of stomata closure causing reduced water loss and thus elevated drought-tolerance. During drought, expression of GUS (β-glucuronidase) under P was remarkably elevated in guard cells of stomata which correlated with its function as a transcription factor regulating stomatal aperture closing. MusaSNAC1 is a transcriptional activator belonging to SNAC subgroup and its 5'-upstream region contain multiple Dof1 elements as well as stress-associated cis-elements. Moreover, MusaSNAC1 also regulate multiple stress-related genes by binding to core site of NAC-proteins CGT[A/G] in their 5'-upstream region. Results indicated an interesting mechanism of drought tolerance through stomatal closure by HO generation in guard cells, regulated by a NAC-protein in banana.

Genome-Wide Association Studies Reveal Genetic Variation and Candidate Genes of Drought Stress Related Traits in Cotton (Gossypium hirsutum L.)

“…Further, we focused on the GO term “response to stress” (SR), which contained 189 candidate genes ( Figure 3A ). Of them, many genes had been reported to play important roles in drought tolerance, such as RD2 , PIP2 , PP2C , and LEA ( Aroca et al, 2006 ; Samota et al, 2017 ; Baek et al, 2018 ; Magwanga et al, 2018 ), and some key transcription factors, WRKY , NAC , and MYB ( Wei et al, 2017 ; Kiranmai et al, 2018 ; Negi et al, 2018 ).…”

Genome-Wide Identification and Characterization of the NAC Transcription Factor Family in Musa Acuminata and Expression Analysis during Fruit Ripening

“…MaNAC genes are also capable of responding to abiotic stress. For instance, MaNAC095 in S2 can promote drought tolerance by modulating stomatal closure and H 2 O 2 content [32], and MaNAC053 in S5 might be involved in responses to higher salinity and drought stresses in banana [37].…”

“…A stress-associated NAC transcription factor, MpSNAC67, from Musa x paradisiaca has been shown to regulate senescence by promoting chlorophyll-catabolic genes [31]. A few vascular-related NAC transcription factors (MusaVND1, MusaVND2, and MusaVND3) have been reported to be efficient regulators of multiple classes of secondary wall-associated genes in banana [32]. The stomatal closure could be induced by a banana NAC protein, MusaSNAC1, by elevating H 2 O 2 content in guard cells during drought stress [32].…”

“…A few vascular-related NAC transcription factors (MusaVND1, MusaVND2, and MusaVND3) have been reported to be efficient regulators of multiple classes of secondary wall-associated genes in banana [32]. The stomatal closure could be induced by a banana NAC protein, MusaSNAC1, by elevating H 2 O 2 content in guard cells during drought stress [32]. Even though many NAC TFs in banana have been identified with respect to their function in fruit development and responses to biotic and abiotic stress, global genomic analysis of NAC genes in banana is still limited to NAC orthologous group analysis [33].…”

Transcriptome changes induced by drought stress in Jerusalem artichoke (Helianthus tuberosus L.) and weighted gene co-expression network analysis (WGCNA)

“…The NAC transcription factor JUB1 in tomato is the drought-tolerant regulator of tomato. SlJUB1 controls the expression of SlDREB1, SlDREB2 and SlDLALA, while AtJUB1 in A. thaliana stimulates the expression of DREB2a and produces drought resistance by controlling the DREB genes [40,41]. DREB2 and DREB2a were also highly expressed in both varieties of Jerusalem artichoke, particularly in QY3, in which the expression of DREB2 was extremely high.…”