A Basic Study on the Biological Monitoring for Vanadium—Effects of Vanadium on Vero Cells and the Evaluation of Intracellular Vanadium Contents

Abstract

A high concentration of vanadium (V) has toxic effects on human and animals and is one of environmental pollutants. In the present study, we have conducted a fundamental study using cultured Vero cells from monkey kidney for the future environmental monitoring. Orthovanadate (VAN), one of V compounds, of 10(-10) and 10(-8) M did not affect the cell growth although the higher concentration of above 10(-6) M VAN inhibited the cell growth accompanied with the decrease in cell numbers and morphological changes. Given that the washing method with ice-cold Li is also effective for determination of the cellular Na content, we used this method for the determination of the V content of the Vero cells. The V distributions in Vero cell; in the 10(-3) M VAN solution, extracellular and intracellular were obtained as 1:0.564:0.036 and 1:0.662:0.098 at 60 and 120 min after the treatment of VAN. The intracellular V content was 10% of the applied concentration of VAN. Consequently, it was suggested that V concentration of 10(-7) and 10(-6) M in the tissue and environment, respectively, might become the threshold concentration; a criterion of the environmental contamination when we carry out environmental monitoring.

The Distribution of Several Elements in Cat Urine and the Relation Between the Content of Elements and Urolithiasis

“…Thus, it was difficult to clarify the state of environmental pollution by comparisons that used only the mean value. Compared to our previous studies of elements using experimental animals [24][25][26] and cultured cell [27], in the study of Listeria monocytogenes in food using molecular epidemiological technique [28,29], a different scientific approach to the study using wildlife was thought to be necessary. Under the circumstances, two new indexes, named the cadmium (Cd) standard regression line (CSRL) [30] and the CSRL by equal probability ellipse (CEPE) [23], were developed to evaluate the degree of pollution of target animals.…”

Vanadium Complex Derived from N’-(3-Bromo-5-chloro-2-hydroxybenzylidene) -3-methylbenzohydrazide: Synthesis, Crystal Structure and Biological Activity

“…2 The element is present in vanadiumcontaining enzymes such as nitrogenase and haloperoxidases. 3 Due to structural similarity between vanadates and phosphate and being able to be transported into cells through the anion channel of the plasma membranes, 4 many vanadium compounds have been shown to increase glucose transport into cells and its oxidation via glycolysis.…”

Synthesis, crystal structure, and insulin-like activity of dimeric vanadium complex derived from 4-bromo-N'-(2-hydroxy-5-methoxybenzylidene)benzohydrazide

“…[1][2][3][4][5] Vanadium is recognized as an essential trace element for different organisms. [6] The element is present in vanadium-containing enzymes such as nitrogenase and haloperoxidases. [7] Due to structural similarity between vanadates and phosphate and being able to be transported into cells through the anion channel of the plasma membranes, [8,9] many vanadium compounds have been shown to increase glucose transport into cells and its oxidation via glycolysis.…”