A bell-shaped dose–response of topical FGF-1 in accelerating dermal wound healing in aged female BALB/cByJ mice

Hagerott, Brooke N.; Blumstein, Alli J.; McGarry, Lauren; Cohen, Hannah M.; Tenorio, Connie A.; Powell, Brett D.; Nagy, Tamás; Blaber, Michael

doi:10.1007/s42485-020-00040-z

Cited by 4 publications

(3 citation statements)

References 33 publications

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“…To further illustrate the differences in scale between the detected quantities of proteins in the studied tenocyte-derived lyophilized APIs and the reported therapeutic doses of the same proteins, selected publications in the field of regenerative medicine presenting the use of individual growth factors were analyzed [50][51][52][53]. Akita et al have reported and discussed the use of FGF-2 at doses of 100 µg/mL in wound healing applications, which correspond to the approved growth factor concentration for skin ulcers in human patients [50].…”

Section: Lyophilized Progenitor Tenocyte Derivative Apis Present Extensive Physicochemical Stability and Contain Low Quantities Of Multipmentioning

confidence: 99%

“…Zhang et al have reported the use of tendon stem cell (i.e., stimulated with 10-80 ng/mL HGF) conditioned medium for the healing promotion of injured Achilles tendons [51]. Hagerott et al have described the treatment of murine dermal wounds using topical FGF-1 in quantities of 0.6-6.0 µg/cm 2 [52]. Finally, Galiano et al have described the topical use of 20 µg of VEGF every other day for five doses on skin full thickness wounds in genetically diabetic mice [53].…”

Section: Lyophilized Progenitor Tenocyte Derivative Apis Present Extensive Physicochemical Stability and Contain Low Quantities Of Multipmentioning

confidence: 99%

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Combination of Hyaluronan and Lyophilized Progenitor Cell Derivatives: Stabilization of Functional Hydrogel Products for Therapeutic Management of Tendinous Tissue Disorders

et al. 2021

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Cultured progenitor cells and derivatives have been used in various homologous applications of cutaneous and musculoskeletal regenerative medicine. Active pharmaceutical ingredients (API) in the form of progenitor cell derivatives such as lysates and lyophilizates were shown to retain function in controlled cellular models of wound repair. On the other hand, hyaluronan-based hydrogels are widely used as functional vehicles in therapeutic products for tendon tissue disorders. The aim of this study was the experimental characterization of formulations containing progenitor tenocyte-derived APIs and hyaluronan, for the assessment of ingredient compatibility and stability in view of eventual therapeutic applications in tendinopathies. Lyophilized APIs were determined to contain relatively low quantities of proteins and growth factors, while being physicochemically stable and possessing significant intrinsic antioxidant properties. Physical and rheological quantifications of the combination formulas were performed after hydrogen peroxide challenge, outlining significantly improved evolutive viscoelasticity values in accelerated degradation settings. Thus, potent effects of physicochemical protection or stability enhancement of hyaluronan by the incorporated APIs were observed. Finally, combination formulas were found to be easily injectable into ex vivo tendon tissues, confirming their compatibility with further translational clinical approaches. Overall, this study provides the technical bases for the development of progenitor tenocyte derivative-based injectable therapeutic products or devices, to potentially be applied in tendinous tissue disorders.

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Section: Lyophilized Progenitor Tenocyte Derivative Apis Present Extensive Physicochemical Stability and Contain Low Quantities Of Multipmentioning

confidence: 99%

Section: Lyophilized Progenitor Tenocyte Derivative Apis Present Extensive Physicochemical Stability and Contain Low Quantities Of Multipmentioning

confidence: 99%

Combination of Hyaluronan and Lyophilized Progenitor Cell Derivatives: Stabilization of Functional Hydrogel Products for Therapeutic Management of Tendinous Tissue Disorders

et al. 2021

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“…To test new spinal implants, the porcine spine is frequently used as a model for the human spine [36]; however, since the spine is mainly vertically loaded in humans, baboons are suggested to be more appropriate models for spinal degeneration [37]. Moreover, growth factors such as FGF-2 show species specificity in their biological activity and bell-shaped dose response, where both an overdose and a deficient dose exhibit diminished or even no effectiveness [38,39]. The FGF-2 used in the present study is a recombinant human protein.…”

Section: Discussionmentioning

confidence: 99%

Safety and Osteointegration of Titanium Screws Coated with a Fibroblast Growth Factor-2–Calcium Phosphate Composite Layer in Non-Human Primates: A Pilot Study

Matsumoto

Mutsuzaki

Hara

et al. 2023

JFB

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Spinal instrumentation surgery for older patients with osteoporosis is increasing. Implant loosening may occur due to inappropriate fixation in osteoporotic bone. Developing implants that achieve stable surgical results, even in osteoporotic bone, can reduce re-operation, lower medical costs, and maintain the physical status of older patients. Fibroblast growth factor-2 (FGF-2) promotes bone formation; thus, coating pedicle screws with an FGF-2–calcium phosphate (FGF-CP) composite layer is hypothesized to enhance osteointegration in spinal implants. We designed a long-term implantation pilot study that estimated the safety and bone-forming efficacy of pedicle screws coated with an FGF-CP composite layer in cynomolgus monkeys. Titanium alloy screws, either uncoated (controls) or aseptically coated with an FGF-CP composite layer, were implanted in the vertebral bodies of six female adult cynomolgus monkeys (three monkeys per group) for 85 days. Physiological, histological, and radiographic investigations were performed. There were no serious adverse events, and no radiolucent areas were observed around the screws in either group. The bone apposition rate in the intraosseous region was significantly higher in the FGF-CP group than in the controls. Moreover, as analyzed by Weibull plots, the bone formation rate of the FGF-CP group exhibited a significantly higher regression line slope than the control group. These results demonstrated that there was significantly less risk of impaired osteointegration in the FGF-CP group. Our pilot study suggests that FGF-CP-coated implants could promote osteointegration, be safe, and reduce the probability of screw loosening.

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Wound Healing Splinting Devices for Faster Access and Use

Miller,

Anderson,

Suarez-Arnedo

et al. 2024

JID Innovations

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A bell-shaped dose–response of topical FGF-1 in accelerating dermal wound healing in aged female BALB/cByJ mice

Cited by 4 publications

References 33 publications

Combination of Hyaluronan and Lyophilized Progenitor Cell Derivatives: Stabilization of Functional Hydrogel Products for Therapeutic Management of Tendinous Tissue Disorders

Combination of Hyaluronan and Lyophilized Progenitor Cell Derivatives: Stabilization of Functional Hydrogel Products for Therapeutic Management of Tendinous Tissue Disorders

Safety and Osteointegration of Titanium Screws Coated with a Fibroblast Growth Factor-2–Calcium Phosphate Composite Layer in Non-Human Primates: A Pilot Study

Wound Healing Splinting Devices for Faster Access and Use

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