A benzooxazine-based ratiometric fluorescent probe for pH imaging in living cells and bacteria

A latent reversible ratiometric optical pH sensing probe based on phenylboronic acid for alkaline pH detection and applications in test paper and alkalotic HK-2 cells

“…The fluorescence quantum yields (F) of DDPB (in ethanol) and the pH-responded product (HDM) (in ethanol) were calculated using eqn (2) with Rhodamine B (f s = 89% in ethanol) as a standard. 30…”

“…Acid–base disorder is a common but often ignored problem in the clinic, 1 while acid–base imbalance largely affects a series of important cell functions, including cellular proliferation, apoptosis, endocytosis and ion transmission. 2 It is well-known that the acid–base balance is strictly regulated by pH. 3 Under normal physiological conditions, the arterial pH value is carefully maintained between 7.36 and 7.44.…”