A bHLH gene NnTT8 of Nelumbo nucifera regulates anthocyanin biosynthesis

Biotechnology & Biotechnological Equipment

Wang

Zhang

et al. 2021

Section: Bhlh Transcription Factorsmentioning

confidence: 99%

Role of core structural genes for flavonoid biosynthesis and transcriptional factors in flower color of plants

Biotechnology & Biotechnological Equipment

Wang

Zhang

et al. 2021

“…The bHLH proteins influence anthocyanin synthesis by directly activating the anthocyanin pathway genes or forming the MBW complex to mediate the structural genes. It was reported that DcTT8 could regulate anthocyanin in Dendrobium candidum through inducing the expression of DcF3′H and DcUFGT [ 16 ], and the same activation pattern was also observed in other higher plants such as Nelumbo nucifera and tree peony ( Paeonia suffruticosa ) [ 17 , 18 ]. What is more, in Arabidopsis , MBW complexes could directly target AtDFR and AtLODX to regulate their transcriptional activity and in Medicago truncatula MtTT8 together with MtWD40-1, they could directly target activated promoters of MtANS to regulate anthocyanin synthesis [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 84%

Comparative Transcriptome Analysis of Purple and Green Non-Heading Chinese Cabbage and Function Analyses of BcTT8 Gene

Tang

Xiao

et al. 2022

Genes

Non-heading Chinese cabbage (Brassica campestris ssp. chinensis) is an important vegetative crop in the south of China. As an antioxidant, anthocyanin is the major quality trait for vegetables with purple leaves or petioles. However, the molecular biosynthetic mechanism of anthocyanin in non-heading Chinese cabbage has not been explained exclusively. In this study, two non-heading Chinese cabbage with contrasting colors in the leaves were used as the materials for RNA-seq. A total of 906 DEGs were detected, and we found that the anthocyanin and flavonoid biosynthetic pathways are significantly enriched in the purple NHCC. The transcriptome result was verified by RT-qPCR. Though bioinformatics analysis, BcTT8 was selected as the candidate gene for the regulation of anthocyanin synthesis, and the characterization of BcTT8 was elucidated by the functional analyses. The results proved that BcTT8 is a nucleus protein and phylogenetically close to the TT8 protein from Brassica. After silencing BcTT8, the total anthocyanin content of pTY-BcTT8 plants decreased by 42.5%, and the relative expression levels of anthocyanin pathway genes BcDFR, BcLODX and BcUF3GT-1 were significantly downregulated, while the transcription level of BcFLS was significantly upregulated. Compared with the wild type, the transgenic Arabidopsis showed obvious violet in the cotyledons part, and the anthocyanin biosynthetic genes such as AtDFR and AtLODX were significantly upregulated. In conclusion, BcTT8 is critical in the anthocyanin synthesis process of non-heading Chinese cabbage. Our findings illustrated the molecular mechanism of anthocyanin biosynthesis in non-heading Chinese cabbage.

“…Numerous previous studies have reported on anthocyanin biosynthesis-associated genes and their role in the regulation of color phenotypes and tolerance to HL stress [ 79 , 80 , 81 ]. The functions of these genes have also been studied in detail using overexpression and/or silencing strategies in Arabidopsis or other plant species, such as apple [ 82 ], grape [ 42 ] and wheat [ 50 ]. Documenting the transcriptional expression of key anthocyanin biosynthesis-related genes may provide further insight into plant response to HL stress.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, the functions of bHLH s in regulating the anthocyanin-biosynthetic pathway have been investigated in various plant species, including apple, grape ( V. davidii ) [ 47 ], sheepgrass ( Leymus chinensis ) [ 48 ], kiwifruit ( Actinidia spp.) [ 49 ], and lotus ( Nelumbo nucifera ) [ 50 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals Roles of Anthocyanin- and Jasmonic Acid-Biosynthetic Pathways in Rapeseed in Response to High Light Stress

Luo

Teng

et al. 2021

IJMS

Rapeseed (Brassica napus) is one of the major important oil crops worldwide and is largely cultivated in the Qinghai-Tibetan plateau (QTP), where long and strong solar-radiation is well-known. However, the molecular mechanisms underlying rapeseed’s response to light stress are largely unknown. In the present study, the color of rapeseed seedlings changed from green to purple under high light (HL) stress conditions. Therefore, changes in anthocyanin metabolism and the transcriptome of rapeseed seedlings cultured under normal light (NL) and HL conditions were analyzed to dissect how rapeseed responds to HL at the molecular level. Results indicated that the contents of anthocyanins, especially glucosides of cyanidin, delphinidin, and petunidin, which were determined by liquid chromatography-mass spectrometry (LC-MS), increased by 9.6-, 4.2-, and 59.7-fold in rapeseed seedlings exposed to HL conditions, respectively. Next, RNA-sequencing analysis identified 7390 differentially expressed genes (DEGs), which included 4393 up-regulated and 2997 down-regulated genes. Among the up-regulated genes, many genes related to the anthocyanin-biosynthetic pathway were enriched. For example, genes encoding dihydroflavonol reductase (BnDFR) and anthocyanin synthase (BnANS) were especially induced by HL conditions, which was also confirmed by RT-qPCR analysis. In addition, two PRODUCTION OF ANTHOCYANIN PIGMENTATION 2 (BnPAP2) and GLABRA3 (BnGL3) genes encoding MYB-type and bHLH-type transcription factors, respectively, whose expression was also up-regulated by HL stress, were found to be associated with the changes in anthocyanin biosynthesis. Many genes involved in the jasmonic acid (JA)-biosynthetic pathway were also up-regulated under HL conditions. This finding, which is in agreement with the well-known positive regulatory role of JA in anthocyanin biosynthesis, suggests that the JA may also play a key role in the responses of rapeseed seedlings to HL. Collectively, these data indicate that anthocyanin biosynthesis-related and JA biosynthesis-related pathways mediate HL responses in rapeseed. These findings collectively provide mechanistic insights into the mechanisms involved in the response of rapeseed to HL stress, and the identified key genes may potentially be used to improve HL tolerance of rapeseed cultivars through genetic engineering or breeding strategies.