A bi-layer electrospun nanofiber membrane for plasmid DNA recovery from fermentation broths

Correia, Tiago Ruivo; Antunes, Bernardo Paiva; Castilho, Pedro Henrique Barata; Nunes, José C.; Amorim, Maria João; Escobar, Isabel C.; Queiroz, João A.; Correia, Ilídio J.; Morão, A.

doi:10.1016/j.seppur.2013.03.049

Cited by 14 publications

(8 citation statements)

References 33 publications

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“…82 The porosity of the lyophilized hydrogels was determined, aer 24 h of immersion in ethanol, using the following eqn (2): The total porosity of the ChMA : GelMA hydrogels was determined by adapting a displacement method previously reported in literature.…”

Section: Methodsmentioning

confidence: 99%

Synthesis and characterization of a photocrosslinkable chitosan–gelatin hydrogel aimed for tissue regeneration

et al. 2015

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In the area of tissue engineering different approaches have been studied, so far, for promoting regeneration or replacement of damaged tissues. Among the different materials developed, hydrogels, due to their biocompatibility and similarities with the native extracellular matrix, have emerged as suitable candidates for being used for different therapeutic purposes. Herein, photocrosslinkable hydrogels, composed by chitosan methacrylamide (ChMA) and gelatin methacrylamide (GelMA) were crosslinked by ultraviolet (UV) light, using Irgacue 2959 as photoinitiator. The morphological, physicochemical and biological properties of the hydrogels were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and nuclear magnetic resonance. The obtained results demonstrated that the developed hydrogels possess suitable properties for being used as 3D constructs on several areas of tissue engineering. Furthermore, these properties may allow their future application as space filling agents or as delivery vehicles of bioactive molecules and cells.

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Section: Methodsmentioning

confidence: 99%

Synthesis and characterization of a photocrosslinkable chitosan–gelatin hydrogel aimed for tissue regeneration

et al. 2015

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“…The membrane porosity method has been previously described . Briefly, the total porosity of the membranes was measured through the determination of the amount of ethanol absorbed by the membranes using the following equation:

P (%) = \frac{W_{2} - W_{1}}{d_{ethanol} V_{membrane}} \times 100

where W 1 is the weight of the dry membrane and W 2 is the weight of the wet membrane, d ethanol the density of the ethanol at room temperature, and V membrane is the volume of the wet membrane, directly determined by immersion.…”

Section: Methodsmentioning

confidence: 99%

“…Herein, pDNA has been extensively investigated, to obtain a highly purified molecules for gene therapy applications and DNA vaccine . The purification of pDNA has already been achieved through the use of different techniques, including chromatography, enzymatic, and membrane processes . Here, microfiltration (MF) and ultrafiltration (UF) membranes have gained special interest, avoiding precipitation of solvents, and centrifugation steps.…”

Section: Introductionmentioning

confidence: 99%

Modification of microfiltration membranes by hydrogel impregnation for pDNA purification

Castilho

Correia

Amorim

et al. 2014

J of Applied Polymer Sci

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The huge efforts for the achievement of highly purified biomolecules are growing every day. A great number of efficient techniques, such as chromatography, are already available in laboratory for separation processes. However, membrane‐based technologies are the best match to assure simplicity, efficiency and easy scale‐up procedures. Herein we report the modification of a commercial microfiltration membrane for plasmid DNA purification by agarose gel impregnation. The membrane was characterized by SEM, ATR‐FTIR, EDS, contact angle, and porosity measurements. Additionally, the membrane pore radius was estimated from observed rejections of different proteins and with that information the rejection of a 6050 bp plasmid DNA (pDNA) molecule was estimated for different values of flux using a theoretical model of large flexible molecules in membranes with parallel cylindrical pores, which is applicable to pDNA ultrafiltration in conventional membranes, as recently shown in the literature. The experimental results show that the modified membrane has higher pDNA rejections than the predicted by the model, suggesting that the different type of porous structure that a hydrogel has, may have a positive effect on pDNA rejections as compared to other biomolecules with more rigid structures, making this type of modified membranes potential better candidates to be used for the selective recovery of pDNA in this type of bioprocesses. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41610.

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“…The porosity ratio (P%) of the selected film, was measured through the determination of the amount of solvent absorbed by the film sample (20 mm x 20 mm) after 1 h of immersion in ethanol, using Eq. 1 (Correia et al, 2013):…”

Section: Porosity Testmentioning

confidence: 99%

Preparation and characterization of a novel antimicrobial film dressing for wound healing application

Gonçalves

Carneiro

Justus

et al. 2020

Braz. J. Pharm. Sci.

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Antibacterial activity and good mechanical properties are some of the characteristics required for an appropriate film dressing. A novel polymer blend was developed for wound healing application. Twenty-four formulations using the polymers chitosan, poly(vinyl alcohol) and/or ɛ-Polylysine and the plasticizer glycerol were designed using factorial design and then the films were prepared by the casting/solvent evaporation method. Seventeen films were obtained among the twenty-four proposed formulations that were characterized by Field Emission Scanning Electron Microscopy (FE-SEM) and Fourier Transform Infrared Spectroscopy (FTIR). Mechanical properties, such as tensile strength (σ), elongation at break (ɛ) and Young's modulus (Y) as well as antibacterial properties were determined. The best candidate was then further analyzed with regard to porosity, Water Vapor Transmission Rate (WVTR), swelling and cytotoxicity experiments. The results showed a film with semi-occlusive characteristics, good mechanical properties and no toxic. Incorporation of ɛ-Polylysine increased antibacterial activity against gram-negative (Escherichia coli) and gram-positive (Staphylococcus aureus) bacteria.

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A bi-layer electrospun nanofiber membrane for plasmid DNA recovery from fermentation broths

Cited by 14 publications

References 33 publications

Synthesis and characterization of a photocrosslinkable chitosan–gelatin hydrogel aimed for tissue regeneration

Synthesis and characterization of a photocrosslinkable chitosan–gelatin hydrogel aimed for tissue regeneration

Modification of microfiltration membranes by hydrogel impregnation for pDNA purification

Preparation and characterization of a novel antimicrobial film dressing for wound healing application

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