A bidirectional antagonism between aPKC and Yurt regulates epithelial cell polarity

Gamblin, Clémence L.; Hardy, Émilie J.-L.; Chartier, François; Bisson, Nicolas; Laprise, Patrick

doi:10.1083/jcb.201308032

Cited by 41 publications

(86 citation statements)

References 31 publications

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“…A key feature of the machinery that regulates epithelial polarity is the negative feedback between apical and basolateral polarity proteins (Tanentzapf and Tepass, 2003;Bilder et al, 2003;Benton and St Johnston, 2003;Laprise et al, 2009;Chartier et al, 2011;Fletcher et al, 2012;Gamblin et al, 2014). This mutual antagonism can be revealed through double mutant analysis.…”

Section: Resultsmentioning

confidence: 99%

The RhoGEF Cysts couples apical polarity proteins to Rho and myosin activity at adherens junctions

Silver

Wirtz-Peitz²,

Simões

et al. 2019

Preprint

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The spatio-temporal regulation of small Rho GTPases is crucial for the dynamic stability of epithelial tissues. However, how RhoGTPase activity is controlled during development remains largely unknown. To explore the regulation of Rho GTPases in vivo we analyzed the Rho GTPase guanine nucleotide exchange factor (RhoGEF) Cysts, the Drosophila orthologue of mammalian p114RhoGEF, GEF-H1, p190RhoGEF, and AKAP-13. Loss of Cysts causes a phenotype that closely resemble the mutant phenotype of the apical polarity regulator Crumbs.This phenotype can be suppressed by the loss of basolateral polarity proteins suggesting that Cysts in an integral component of the apical polarity protein network. Cysts activates Rho at adherens junctions to promote junctional enrichment of myosin II, which requires the RhoGEF domain and the coiled-coil domain containing C-terminal region of Cysts. Cysts recruitment to the apico-lateral cortex depends on Crumbs and Bazooka/Par3 and requires multiple domains within Cysts including the C-terminal region. Together, our findings indicate that Cysts links apical polarity proteins to Rho1 and myosin activation at adherens junctions to support junctional and epithelial integrity in the Drosophila ectoderm.

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Section: Resultsmentioning

confidence: 99%

The RhoGEF Cysts couples apical polarity proteins to Rho and myosin activity at adherens junctions

Silver

Wirtz-Peitz²,

Simões

et al. 2019

Preprint

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show abstract

“…aPKC can also act on basolateral determinants (Figure C), phosphorylating Par1 , Lgl and Yurt to prevent their anchoring at the (often apical) membrane. Although the exact mechanisms that result in this are not always clear, one route is via phosphorylation promoting their association with the 14‐3‐3 protein Par5 in the cytosol .…”

Section: Competition and Cooperation Between Polarity Proteinsmentioning

confidence: 99%

Complex Polarity: Building Multicellular Tissues Through Apical Membrane Traffic

Román-Fernández

Bryant

2016

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The formation of distinct subdomains of the cell surface is crucial for multicellular organism development. The most striking example of this is apical-basal polarization. What is much less appreciated is that underpinning an asymmetric cell surface is an equally dramatic intracellular endosome rearrangement. Here, we review the interplay between classical cell polarity proteins and membrane trafficking pathways, and discuss how this marriage gives rise to cell polarization. We focus on those mechanisms that regulate apical polarization, as this is providing a number of insights into how membrane traffic and polarity are regulated at the tissue level.

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“…Embryos were heat-fixed in E-wash buffer (7% NaCl, 0.5% Triton X-100) at 80°C, which was immediately cooled down by addition of ice-cold E-wash (Gamblin et al, 2014). Embryos were then rinsed with PBS and placed in methanol under a heptane phase, devitellinized by strong agitation and further incubated for 1 h in fresh methanol.…”

Section: Immunofluorescencementioning

confidence: 99%

Girdin-mediated interactions between cadherin and the actin cytoskeleton are required for epithelial morphogenesis in Drosophila

2015

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A bidirectional antagonism between aPKC and Yurt regulates epithelial cell polarity

Abstract: During epithelial cell polarization, aPKC phosphorylates Yurt to prevent its premature apical localization, while at the same time Yurt binds to and restrains aPKC function.

Cited by 41 publications

References 31 publications

The RhoGEF Cysts couples apical polarity proteins to Rho and myosin activity at adherens junctions

The RhoGEF Cysts couples apical polarity proteins to Rho and myosin activity at adherens junctions

Complex Polarity: Building Multicellular Tissues Through Apical Membrane Traffic

Girdin-mediated interactions between cadherin and the actin cytoskeleton are required for epithelial morphogenesis in Drosophila

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