A Bifidobacterium mixed-species microarray for high resolution discrimination between intestinal bifidobacteria

Boesten, Rolf; Schuren, Frank; Vos, Willem M. de

doi:10.1016/j.mimet.2008.12.006

Cited by 24 publications

(16 citation statements)

References 45 publications

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“…Transcriptomes of the fecal bifidobacterial populations. To gain insight into the activities of the bifidobacterial populations within the fecal microbiotas, transcripts were profiled using a mixed-species microarray of bifidobacteria (7). In parallel to the fecal microbial diversity analysis, samples from the same five partly age-matched infants (infants 1 to 5; see Materials and Methods) with specified diets (breast milk or formula) were compared using the microarray at three time points in a 2-to 7-week period.…”

Section: Resultsmentioning

confidence: 99%

Mixed-Species Genomic Microarray Analysis of Fecal Samples Reveals Differential Transcriptional Responses of Bifidobacteria in Breast- and Formula-Fed Infants

Klaassens

Boesten

Haarman

et al. 2009

Appl Environ Microbiol

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Although their exact function remains enigmatic, bifidobacteria are among the first colonizers of the newborn infant gut and further develop into abundant communities, notably in response to diet. Therefore, the transcriptional responses of bifidobacteria in rapidly processed fecal samples from young infants that were fed either breast milk or a formula containing a mixture of galacto-and fructo-oligosaccharides were studied. The presence and diversity of the bifidobacterial fecal communities were determined using PCR-denaturing gradient gel electrophoresis and quantitative real-time PCR for specific species. Changes in the total number of bifidobacteria as well as in species diversity were observed, indicating the metabolic activities of the bifidobacteria within the infant gut. In addition, total RNAs isolated from infant feces were labeled and hybridized to a bifidobacterium-specific microarray comprising approximately 6,000 clones of the major bifidobacterial species of the human gut. Approximately 270 clones that showed the most prominent hybridization with the samples were sequenced. Fewer than 10% of the hybridizing clones contained rRNA genes, whereas the vast majority of the inserts showed matches with protein-encoding genes predicted to originate from bifidobacteria. Although a wide range of functional groups was covered by the obtained sequences, the largest fraction (14%) of the transcribed genes assigned to a functional category were predicted to be involved in carbohydrate metabolism, while some were also implicated in exopolysaccharide production or folate production. A total of three of the above-described protein-encoding genes were selected for quantitative PCR and sequence analyses, which confirmed the expression of the corresponding genes and the expected nucleotide sequences. In conclusion, the results of this study show the feasibility of obtaining insight into the transcriptional responses of intestinal bifidobacteria by analyzing fecal RNA and highlight the in vivo expression of bifidobacterial genes implicated in host-related functions.

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Section: Resultsmentioning

confidence: 99%

Mixed-Species Genomic Microarray Analysis of Fecal Samples Reveals Differential Transcriptional Responses of Bifidobacteria in Breast- and Formula-Fed Infants

Klaassens

Boesten

Haarman

et al. 2009

Appl Environ Microbiol

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“…Thirty-nine species have been assigned to the genus Bifidobacterium, including recent additions such as Bifidobacterium tsurumiense [91] isolated from hamster dental plaque, Bifidobacterium mongoliense isolated from airag, a traditional Mongolian fermented horse milk product [160], Bifidobacterium crudilactis extracted from French raw milk and raw milk cheeses [20], Bifidobacterium psychraerophilum, originating from pig intestine [128], and three bifidobacterial species, Bifidobacterium bombi [52], Bifidobacterium actinicoloniformis and Bifidobacterium bohemicus [51], isolated from the digestive tract of different bumblebee species (Table 1). Furthermore, it seems that many bifidobacteria are still to be discovered as suggested by various metagenomic studies [11,137]. A number of such bifidobacterial strains have been commercially exploited and are usually available on the market as functional components of dairy-based probiotic drinks ( Table 2).…”

Section: Bifidobacteriamentioning

confidence: 99%

Carbohydrate metabolism in Bifidobacteria

2011

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Members of the genus Bifidobacterium can be found as components of the gastrointestinal microbiota, and are believed to play an important role in maintaining and promoting human health by eliciting a number of beneficial properties. Bifidobacteria can utilize a diverse range of dietary carbohydrates that escape degradation in the upper parts of the intestine, many of which are plantderived oligo-and polysaccharides. The gene content of a bifidobacterial genome reflects this apparent metabolic adaptation to a complex carbohydrate-rich gastrointestinal tract environment as it encodes a large number of predicted carbohydrate-modifying enzymes. Different bifidobacterial strains may possess different carbohydrate utilizing abilities, as established by a number of studies reviewed here. Carbohydrate-degrading activities described for bifidobacteria and their relevance to the deliberate enhancement of number and/or activity of bifidobacteria in the gut are also discussed in this review.

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“…numbers were found higher in children who exhibited a normal weight from birth till the age of 7 years in relation to children who became overweight [32], and is it now well known that Bifidobacterium spp. presence is often associated with beneficial health effects [33–35]. More importantly, the authors [32] observed that the Staphylococcus aureus levels were lower in children who maintained a normal weight than in children who became overweight several years later and thus proposed that the protection from obesity seen with bifidobacteria may, in part, be due to its anti-inflammatory effects, whereas S. aureus may trigger low-grade inflammation [36], leading to the overweight status [37, 38].…”

Section: Obesity and Microbiotamentioning

confidence: 99%

Obesity as a Consequence of Gut Bacteria and Diet Interactions

Kotzampassi

Giamarellos‐Bourboulis

Stavrou

2014

ISRN Obesity

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Obesity is a major public health concern, caused by a combination of increased consumption of energy-dense foods and reduced physical activity, with contributions from host genetics, environment, and adipose tissue inflammation. In recent years, the gut microbiome has also been found to be implicated and augmented research in mice and humans have attributed to it both the manifestation and/or exacerbation of this major epidemic and vice versa. At the experimental level, analysis of fecal samples revealed a potential link between obesity and alterations in the gut flora (drop in Bacteroidetes and increase in Firmicutes), the specific gut microbiome being associated with the obese phenotype. Conventionally raised mice were found to have over 40% more total body fat compared with those raised under germ-free conditions, while conventionalization of germ-free mice resulted in a significant increase in total body fat. Similarly, the sparse data in humans supports the fact that fat storage is favoured by the presence of the gut microbiota, through a multifaceted mechanism. Efforts to identify new therapeutic strategies to modulate gut microbiota would be of high priority for public health, and to date, probiotics and/or prebiotics seem to be the most effective tools.

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A Bifidobacterium mixed-species microarray for high resolution discrimination between intestinal bifidobacteria

Cited by 24 publications

References 45 publications

Mixed-Species Genomic Microarray Analysis of Fecal Samples Reveals Differential Transcriptional Responses of Bifidobacteria in Breast- and Formula-Fed Infants

Mixed-Species Genomic Microarray Analysis of Fecal Samples Reveals Differential Transcriptional Responses of Bifidobacteria in Breast- and Formula-Fed Infants

Carbohydrate metabolism in Bifidobacteria

Obesity as a Consequence of Gut Bacteria and Diet Interactions

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